The cryo-EM structure of TdpAB in complex with AMPPNP and PT-DNA
An T, Tan Q, Jiang L, Liu L, Jiang X, Liu L, Chang X, Tian X, Deng Z, Gao S, Wang L, Chen S
Nat Chem Biol (2025) [ DOI: doi:10.1038/s41589-024-01832-w Pubmed: 39820821 ]
- Tdpa (66 kDa, Protein from Thermus antranikianii DSM 12462)
- Dna (5'-d(p*tp*tp*gp*cp*ap*ap*ap*ap*gp*gp*gp*c)-3') (3 kDa, DNA from Thermus antranikianii DSM 12462)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Tdpb (42 kDa, Protein from Thermus antranikianii DSM 12462)
- Dna (5'-d(p*gp*cp*cp*cp*tp*tp*tp*tp*gp*cp*ap*a)-3') (3 kDa, DNA from Thermus antranikianii DSM 12462)
- Tdpab in complex with amppnp and pt-dna (Complex from Thermus antranikianii DSM 12462)
The cryo-EM structure of TdpAB complex
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 8wet
Q-score: 0.562
An T, Tan Q, Jiang L, Liu L, Jiang X, Liu L, Chang X, Tian X, Deng Z, Gao S, Wang L, Chen S
Nat Chem Biol (2025) [ DOI: doi:10.1038/s41589-024-01832-w Pubmed: 39820821 ]
- Tdpab complex (Complex from Thermus antranikianii DSM 12462)
- Tdpb (42 kDa, Protein from Thermus antranikianii DSM 12462)
- Tdpa (66 kDa, Protein from Thermus antranikianii DSM 12462)
The cryo-EM structure of TdpAB in complex with AMPPNP and DNA
Resolution: 2.67 Å
EM Method: Single-particle
Fitted PDBs: 8wfd
Q-score: 0.599
An T, Tan Q, Jiang L, Liu L, Jiang X, Liu L, Chang X, Tian X, Deng Z, Gao S, Wang L, Chen S
Nat Chem Biol (2025) [ DOI: doi:10.1038/s41589-024-01832-w Pubmed: 39820821 ]
- Tdpab in complex with amppnp and dna (Complex from Thermus antranikianii DSM 12462)
- Tdpa (66 kDa, Protein from Thermus antranikianii DSM 12462)
- Dna (5'-d(p*tp*tp*gp*cp*ap*ap*ap*ap*gp*gp*gp*c)-3') (3 kDa, DNA from Thermus antranikianii DSM 12462)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Tdpb (42 kDa, Protein from Thermus antranikianii DSM 12462)
- Dna (5'-d(p*gp*cp*cp*cp*tp*tp*tp*tp*gp*cp*ap*a)-3') (3 kDa, DNA from Thermus antranikianii DSM 12462)
Cryo-EM structure of a class T GPCR in apo state
Resolution: 3.01 Å
EM Method: Single-particle
Fitted PDBs: 7xp4
Q-score: 0.428
Xu W, Wu L, Liu S, Liu X, Cao X, Zhou C, Zhang J, Fu Y, Guo Y, Wu Y, Tan Q, Wang L, Liu J, Jiang L, Fan Z, Pei Y, Yu J, Cheng J, Zhao S, Hao X, Liu ZJ, Hua T
Science (2022) 377 pp. 1298-1304 [ DOI: doi:10.1126/science.abo1633 Pubmed: 36108005 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(t) subunit alpha-3 (30 kDa, Protein from Homo sapiens)
- Endoglucanase h,taste receptor type 2 member 46,endoglucanase h,taste receptor type 2 member 46,bitter taste receptor t2r46 (91 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Complex of gpcr and g protein (Complex from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Homo sapiens)
Cryo-EM structure of a class T GPCR in ligand-free state
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 7xp5
Q-score: 0.412
Xu W, Wu L, Liu S, Liu X, Cao X, Zhou C, Zhang J, Fu Y, Guo Y, Wu Y, Tan Q, Wang L, Liu J, Jiang L, Fan Z, Pei Y, Yu J, Cheng J, Zhao S, Hao X, Liu ZJ, Hua T
Science (2022) 377 pp. 1298-1304 [ DOI: doi:10.1126/science.abo1633 Pubmed: 36108005 ]
- Endoglucanase h,taste receptor type 2 member 46,bitter taste receptor t2r46 (91 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(t) subunit alpha-3 (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Complex of gpcr and g protein (Complex from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Homo sapiens)
Cryo-EM structure of a class T GPCR in active state
Resolution: 3.01 Å
EM Method: Single-particle
Fitted PDBs: 7xp6
Q-score: 0.415
Xu W, Wu L, Liu S, Liu X, Cao X, Zhou C, Zhang J, Fu Y, Guo Y, Wu Y, Tan Q, Wang L, Liu J, Jiang L, Fan Z, Pei Y, Yu J, Cheng J, Zhao S, Hao X, Liu ZJ, Hua T
Science (2022) 377 pp. 1298-1304 [ DOI: doi:10.1126/science.abo1633 Pubmed: 36108005 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(t) subunit alpha-3 (30 kDa, Protein from Homo sapiens)
- Endoglucanase h,taste receptor type 2 member 46,endoglucanase h,taste receptor type 2 member 46,bitter taste receptor t2r46 (91 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Complex of gpcr and g protein (Complex from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Homo sapiens)
- Strychnine (334 Da, Ligand)