Glutamate synthase, glutamate dehydrogenase counter-enzyme complex
Resolution: 2.42 Å
EM Method: Single-particle
Fitted PDBs: 7mfm
Q-score: 0.558
Jayaraman V, Lee DJ, Elad N, Vimer S, Sharon M, Fraser JS, Tawfik DS
Nat Chem Biol (2022) 18 pp. 161-170 [ Pubmed: 34931064 DOI: doi:10.1038/s41589-021-00919-y ]
- Flavin-adenine dinucleotide (785 Da, Ligand)
- Glutamate dehydrogenase (47 kDa, Protein from Bacillus subtilis)
- Flavin mononucleotide (456 Da, Ligand)
- Glutamate synthase (nadph) large chain (169 kDa, Protein from Bacillus subtilis)
- Iron/sulfur cluster (351 Da, Ligand)
- Fe3-s4 cluster (295 Da, Ligand)
- Gudb6-glta2-gltb2 (740 kDa, Complex from Bacillus subtilis)
- Glutamate synthase (nadph) small chain (58 kDa, Protein from Bacillus subtilis)
Glutamate synthase, glutamate dehydrogenase counter-enzyme complex (GudB6-GltA6-GltB6)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7mft
Q-score: 0.397
Jayaraman V, Lee DJ, Elad N, Vimer S, Sharon M, Fraser JS, Tawfik DS
Nat Chem Biol (2022) 18 pp. 161-170 [ DOI: doi:10.1038/s41589-021-00919-y Pubmed: 34931064 ]
- Gudb-glta-gltb (1 MDa, Complex from Bacillus subtilis)
- Glutamate synthase (nadph) large chain (169 kDa, Protein from Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10)
- Fe3-s4 cluster (295 Da, Ligand)
- Glutamate synthase (nadph) small chain (58 kDa, Protein from Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10)
- Glutamate dehydrogenase (47 kDa, Protein from Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10)
- Flavin-adenine dinucleotide (785 Da, Ligand)
- Flavin mononucleotide (456 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
Cfr-modified 50S subunit from Escherichia coli
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 7lvk
Q-score: 0.646
Tsai K, Stojkovic V, Noda-Garcia L, Myasnikov AG, Young ID, Palla A, Venkataramanan S, Floor S, Fraser JS, Frost A, Tawfik DS, Fujimori DG
To Be Published
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli)
- Water (18 Da, Ligand)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
- 5s rrna (38 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
- Escherichia coli 50s subunit (Complex from Escherichia coli)
- Sodium ion (22 Da, Ligand)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
- 23s rrna (941 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)