Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 8r6u
Q-score: 0.565
Williams HM, Thorkelsson SR, Vogel D, Busch C, Milewski M, Cusack S, Grunewald K, Quemin ERJ, Rosenthal M
Nucleic Acids Res (2024) 52 pp. 6049-6065 [ DOI: doi:10.1093/nar/gkae330 Pubmed: 38709882 ]
- Rna primer tail-end (5 kDa, RNA from SFTS virus AH12)
- Magnesium ion (24 Da, Ligand)
- Sftsv l protein in complex with 5' and 3' rna, as well as rna acting as a primer. (240 kDa, Complex from SFTS virus AH12)
- Rna primer (6 kDa, RNA from SFTS virus AH12)
- Rna-directed rna polymerase l (235 kDa, Protein from SFTS virus AH12)
- Rna (5'-r(p*cp*up*gp*gp*gp*cp*gp*gp*up*cp*up*up*up*gp*up*gp*u)-3') (8 kDa, RNA from SFTS virus AH12)
Structure of the SFTSV L protein stalled at early elongation [EARLY-ELONGATION]
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8as7
Q-score: 0.582
Williams HM, Thorkelsson SR, Vogel D, Milewski M, Busch C, Cusack S, Grunewald K, Quemin ERJ, Rosenthal M
Nucleic Acids Res (2023) 51 pp. 1424-1442 [ Pubmed: 36651274 DOI: doi:10.1093/nar/gkac1249 ]
- Magnesium ion (24 Da, Ligand)
- Rna-dependent rna-polymerase l protein (Complex from SFTS virus AH12)
- Water (18 Da, Ligand)
- Rna (5'-r(p*ap*cp*a)-3') (Complex from SFTS virus AH12)
- Rna-dependent rna-polymerase l protein (235 kDa, Protein from SFTS virus AH12)
- Rna (5'-r(*ap*cp*ap*cp*ap*gp*ap*gp*ap*cp*gp*cp*cp*cp*ap*gp*a)-3') (6 kDa, RNA from SFTS virus AH12)
- 5'-o-[(s)-hydroxy{[(s)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]uridine (483 Da, Ligand)
- Rna (5'-r(p*cp*up*gp*gp*gp*cp*gp*gp*up*cp*up*up*up*gp*up*gp*u)-3') (Complex from SFTS virus AH12)
- Rna (5'-r(p*cp*up*gp*gp*gp*cp*gp*gp*up*cp*up*up*up*gp*up*gp*u)-3') (8 kDa, RNA from SFTS virus AH12)
- 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (238 Da, Ligand)
- Sftsv l protein stalled at early elongation [early-elongation] (238 kDa, Complex)
- Rna (5'-r(*ap*cp*ap*cp*ap*gp*ap*gp*ap*cp*gp*cp*cp*cp*ap*gp*a)-3') (Complex from SFTS virus AH12)
- Rna (5'-r(p*ap*cp*a)-3') (8 kDa, RNA from SFTS virus AH12)
Structure of the SFTSV L protein stalled at late elongation [LATE-ELONGATION]
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8asd
Q-score: 0.623
Williams HM, Thorkelsson SR, Vogel D, Milewski M, Busch C, Cusack S, Grunewald K, Quemin ERJ, Rosenthal M
Nucleic Acids Res (2023) 51 pp. 1424-1442 [ Pubmed: 36651274 DOI: doi:10.1093/nar/gkac1249 ]
- Magnesium ion (24 Da, Ligand)
- Rna-dependent rna-polymerase l protein (Complex from SFTS virus AH12)
- Water (18 Da, Ligand)
- Structure of the sftsv l protein stalled at late elongation [late-elongation] (238 kDa, Complex)
- Rna (5'-r(p*ap*cp*ap*cp*ap*ap*ap*gp*ap*cp*cp*gp*cp*cp*cp*ap*gp*ap*cp*cp*up*up*up*up*up*u)-3') (8 kDa, RNA from SFTS virus AH12)
- Rna (5'-r(p*ap*cp*ap*cp*ap*gp*ap*gp*ap*cp*gp*cp*cp*cp*ap*gp*ap*up*gp*a)-3') (6 kDa, RNA from SFTS virus AH12)
- Rna (Complex from SFTS virus AH12)
- 5'-o-[(s)-hydroxy{[(s)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]uridine (483 Da, Ligand)
- Rna-directed rna polymerase (235 kDa, Protein from SFTS virus AH12)
- Rna (5'-r(p*ap*ap*ap*ap*ap*ap*gp*ap*up*cp*up*gp*gp*gp*cp*gp*gp*up*cp*up*up*up*gp*up*gp*u)-3') (8 kDa, RNA from SFTS virus AH12)
Lassa virus L protein bound to 3' promoter RNA (well-resolved polymerase core) [3END-CORE]
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7oea
Q-score: 0.51
Kouba T, Vogel D, Thorkelsson SR, Quemin ERJ, Williams HM, Milewski M, Busch C, Gunther S, Grunewald K, Rosenthal M, Cusack S
Nat Commun (2021) 12 pp. 7018-7018 [ Pubmed: 34857749 DOI: doi:10.1038/s41467-021-27305-5 ]
- 3' vrna (5 kDa, RNA from Lassa mammarenavirus)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Rna-directed rna polymerase l (253 kDa, Protein from Lassa mammarenavirus)
- Zinc ion (65 Da, Ligand)
- Rna-directed rna polymerase l lassa mammarenavirus (253 kDa, Complex from Lassa mammarenavirus)
Lassa virus L protein in an elongation conformation [ELONGATION]
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 7ojn
Q-score: 0.531
Kouba T, Vogel D, Thorkelsson SR, Quemin ERJ, Williams HM, Milewski M, Busch C, Gunther S, Grunewald K, Rosenthal M, Cusack S
Nat Commun (2021) 12 pp. 7018-7018 [ Pubmed: 34857749 DOI: doi:10.1038/s41467-021-27305-5 ]
- Product rna (2 kDa, RNA from Lassa mammarenavirus)
- Manganese (ii) ion (54 Da, Ligand)
- Product rna (Complex from Lassa mammarenavirus)
- 5' rna (6 kDa, RNA from Lassa mammarenavirus)
- Rna-directed rna polymerase l (253 kDa, Protein from Lassa mammarenavirus)
- Rna-directed rna polymerase l (Complex from Lassa mammarenavirus)
- Zinc ion (65 Da, Ligand)
- 5'-o-[(s)-hydroxy{[(s)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]uridine (483 Da, Ligand)
- 3' rna (6 kDa, RNA from Lassa mammarenavirus)
- 5' rna, 3' rna (Complex from Lassa mammarenavirus)
- Rna-directed rna polymerase l lassa mammarenavirus (253 kDa, Complex)