The cryo-EM structure of TdpAB in complex with AMPPNP and PT-DNA
An T, Tan Q, Jiang L, Liu L, Jiang X, Liu L, Chang X, Tian X, Deng Z, Gao S, Wang L, Chen S
Nat Chem Biol (2025) [ Pubmed: 39820821 DOI: doi:10.1038/s41589-024-01832-w ]
- Tdpab in complex with amppnp and pt-dna (Complex from Thermus antranikianii DSM 12462)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Tdpb (42 kDa, Protein from Thermus antranikianii DSM 12462)
- Dna (5'-d(p*tp*tp*gp*cp*ap*ap*ap*ap*gp*gp*gp*c)-3') (3 kDa, DNA from Thermus antranikianii DSM 12462)
- Tdpa (66 kDa, Protein from Thermus antranikianii DSM 12462)
- Dna (5'-d(p*gp*cp*cp*cp*tp*tp*tp*tp*gp*cp*ap*a)-3') (3 kDa, DNA from Thermus antranikianii DSM 12462)
Cryo-EM reveals cholesterol binding in the lysosomal GPCR-like protein LYCHOS
Zhao J, Shen Q, Yong X, Li X, Tian X, Sun S, Xu Z, Zhang X, Zhang L, Yang H, Shao Z, Xu H, Jiang Y, Zhang Y, Yan W
Nat Struct Mol Biol (2025) [ Pubmed: 39824976 DOI: doi:10.1038/s41594-024-01470-9 ]
- Lysosomal cholesterol signaling protein (93 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Cholesterol-bound lychos (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- [(2r)-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] octadecanoate (720 Da, Ligand)
- Sodium ion (22 Da, Ligand)
The cryo-EM structure of TdpAB complex
An T, Tan Q, Jiang L, Liu L, Jiang X, Liu L, Chang X, Tian X, Deng Z, Gao S, Wang L, Chen S
Nat Chem Biol (2025) [ Pubmed: 39820821 DOI: doi:10.1038/s41589-024-01832-w ]
- Tdpb (42 kDa, Protein from Thermus antranikianii DSM 12462)
- Tdpab complex (Complex from Thermus antranikianii DSM 12462)
- Tdpa (66 kDa, Protein from Thermus antranikianii DSM 12462)
The cryo-EM structure of TdpAB in complex with AMPPNP and DNA
An T, Tan Q, Jiang L, Liu L, Jiang X, Liu L, Chang X, Tian X, Deng Z, Gao S, Wang L, Chen S
Nat Chem Biol (2025) [ Pubmed: 39820821 DOI: doi:10.1038/s41589-024-01832-w ]
- Tdpab in complex with amppnp and dna (Complex from Thermus antranikianii DSM 12462)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Tdpb (42 kDa, Protein from Thermus antranikianii DSM 12462)
- Dna (5'-d(p*tp*tp*gp*cp*ap*ap*ap*ap*gp*gp*gp*c)-3') (3 kDa, DNA from Thermus antranikianii DSM 12462)
- Tdpa (66 kDa, Protein from Thermus antranikianii DSM 12462)
- Dna (5'-d(p*gp*cp*cp*cp*tp*tp*tp*tp*gp*cp*ap*a)-3') (3 kDa, DNA from Thermus antranikianii DSM 12462)
Qian Y, Ma Z, Xu Z, Duan Y, Xiong Y, Xia R, Zhu X, Zhang Z, Tian X, Yin H, Liu J, Song J, Lu Y, Zhang A, Guo C, Jin L, Kim WJ, Ke J, Xu F, Huang Z, He Y
Nat Commun (2024) 15 pp. 7644-7644 [ DOI: doi:10.1038/s41467-024-52174-z Pubmed: 39223191 ]
- Cholesterol hemisuccinate (486 Da, Ligand)
- Gpcr complex (450 kDa, Complex from Homo sapiens)
- Segment polarity protein dishevelled homolog dvl-2 (79 kDa, Protein from Homo sapiens)
- Frizzled-4 (60 kDa, Protein from Homo sapiens)
Fzd4/DEP complex (local refined)
Qian Y, Ma Z, Xu Z, Duan Y, Xiong Y, Xia R, Zhu X, Zhang Z, Tian X, Yin H, Liu J, Song J, Lu Y, Zhang A, Guo C, Jin L, Kim WJ, Ke J, Xu F, Huang Z, He Y
Nat Commun (2024) 15 pp. 7644-7644 [ DOI: doi:10.1038/s41467-024-52174-z Pubmed: 39223191 ]
- Gpcr complex (450 kDa, Complex from Homo sapiens)
- Segment polarity protein dishevelled homolog dvl-2 (79 kDa, Protein from Homo sapiens)
- Frizzled-4 (60 kDa, Protein from Homo sapiens)
Human TOM complex with whole Tom20
Resolution: 5.92 Å
EM Method: Single-particle
Fitted PDBs: 8xva
Q-score: 0.225
Su J, Tian X, Wang Z, Yang J, Sun S, Sui SF
Pnas Nexus (2024) 3 pp. pgae269-pgae269 [ Pubmed: 39071881 DOI: doi:10.1093/pnasnexus/pgae269 ]
- Tom complex with whole tom20 (168 MDa, Complex from Homo sapiens)
- Mitochondrial import receptor subunit tom6 homolog (8 kDa, Protein from Homo sapiens)
- Mitochondrial import receptor subunit tom40 homolog (37 kDa, Protein from Homo sapiens)
- Mitochondrial import receptor subunit tom22 homolog (15 kDa, Protein from Homo sapiens)
- Mitochondrial import receptor subunit tom5 homolog (6 kDa, Protein from Homo sapiens)
- Mitochondrial import receptor subunit tom7 homolog (6 kDa, Protein from Homo sapiens)
- Mitochondrial import receptor subunit tom20 homolog (16 kDa, Protein from Homo sapiens)
Su J, Tian X, Wang Z, Yang J, Sun S, Sui SF
Pnas Nexus (2024) 3 pp. pgae269-pgae269 [ DOI: doi:10.1093/pnasnexus/pgae269 Pubmed: 39071881 ]
- Tom complex with whole tom20 (168 MDa, Complex from Homo sapiens)
Su J, Tian X, Wang Z, Yang J, Sun S, Sui SF
Pnas Nexus (2024) 3 pp. pgae269-pgae269 [ DOI: doi:10.1093/pnasnexus/pgae269 Pubmed: 39071881 ]
- Tom complex with whole tom20 (168 MDa, Complex from Homo sapiens)
3-Methylcrotonyl-CoA Carboxylase in MCCD state with Acetyl CoA
Su J, Tian X, Cheng H, Liu D, Wang Z, Sun S, Wang HW, Sui SF
Nat Struct Mol Biol (2024) [ Pubmed: 39223421 DOI: doi:10.1038/s41594-024-01379-3 ]
- Dodecamer of human mcc (Complex from Homo sapiens)
- 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal (228 Da, Ligand)
- Acetyl coenzyme *a (809 Da, Ligand)
- Methylcrotonoyl-coa carboxylase beta chain, mitochondrial (61 kDa, Protein from Homo sapiens)
- Methylcrotonoyl-coa carboxylase subunit alpha, mitochondrial (80 kDa, Protein from Homo sapiens)