The cryo-EM structure of TdpAB in complex with AMPPNP and PT-DNA
An T, Tan Q, Jiang L, Liu L, Jiang X, Liu L, Chang X, Tian X, Deng Z, Gao S, Wang L, Chen S
Nat Chem Biol (2025) [ DOI: doi:10.1038/s41589-024-01832-w Pubmed: 39820821 ]
- Tdpa (66 kDa, Protein from Thermus antranikianii DSM 12462)
- Dna (5'-d(p*tp*tp*gp*cp*ap*ap*ap*ap*gp*gp*gp*c)-3') (3 kDa, DNA from Thermus antranikianii DSM 12462)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Tdpb (42 kDa, Protein from Thermus antranikianii DSM 12462)
- Dna (5'-d(p*gp*cp*cp*cp*tp*tp*tp*tp*gp*cp*ap*a)-3') (3 kDa, DNA from Thermus antranikianii DSM 12462)
- Tdpab in complex with amppnp and pt-dna (Complex from Thermus antranikianii DSM 12462)
Cryo-EM reveals cholesterol binding in the lysosomal GPCR-like protein LYCHOS
Zhao J, Shen Q, Yong X, Li X, Tian X, Sun S, Xu Z, Zhang X, Zhang L, Yang H, Shao Z, Xu H, Jiang Y, Zhang Y, Yan W
Nat Struct Mol Biol (2025) [ Pubmed: 39824976 DOI: doi:10.1038/s41594-024-01470-9 ]
- [(2r)-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] octadecanoate (720 Da, Ligand)
- Water (18 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Lysosomal cholesterol signaling protein (93 kDa, Protein from Homo sapiens)
- Cholesterol-bound lychos (Complex from Homo sapiens)
The cryo-EM structure of TdpAB complex
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 8wet
Q-score: 0.562
An T, Tan Q, Jiang L, Liu L, Jiang X, Liu L, Chang X, Tian X, Deng Z, Gao S, Wang L, Chen S
Nat Chem Biol (2025) [ DOI: doi:10.1038/s41589-024-01832-w Pubmed: 39820821 ]
- Tdpab complex (Complex from Thermus antranikianii DSM 12462)
- Tdpb (42 kDa, Protein from Thermus antranikianii DSM 12462)
- Tdpa (66 kDa, Protein from Thermus antranikianii DSM 12462)
The cryo-EM structure of TdpAB in complex with AMPPNP and DNA
Resolution: 2.67 Å
EM Method: Single-particle
Fitted PDBs: 8wfd
Q-score: 0.599
An T, Tan Q, Jiang L, Liu L, Jiang X, Liu L, Chang X, Tian X, Deng Z, Gao S, Wang L, Chen S
Nat Chem Biol (2025) [ DOI: doi:10.1038/s41589-024-01832-w Pubmed: 39820821 ]
- Tdpab in complex with amppnp and dna (Complex from Thermus antranikianii DSM 12462)
- Tdpa (66 kDa, Protein from Thermus antranikianii DSM 12462)
- Dna (5'-d(p*tp*tp*gp*cp*ap*ap*ap*ap*gp*gp*gp*c)-3') (3 kDa, DNA from Thermus antranikianii DSM 12462)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Tdpb (42 kDa, Protein from Thermus antranikianii DSM 12462)
- Dna (5'-d(p*gp*cp*cp*cp*tp*tp*tp*tp*gp*cp*ap*a)-3') (3 kDa, DNA from Thermus antranikianii DSM 12462)
Cryo-EM structure of CB2-G protein complex
Yang X, Zhou P, Shen S, Hu Q, Tian C, Xia A, Wang Y, Yang Z, Nan J, Zhou Y, Chen S, Tian X, Wu C, Lin G, Zhang L, Wang K, Zheng T, Zou J, Yan W, Shao Z, Yang S
PNAS (2024) 121 pp. e2401091121-e2401091121 [ DOI: doi:10.1073/pnas.2401091121 Pubmed: 39024109 ]
- Yl025-bound cb2r in complex with gi heterotrimer (Complex)
- Scfv16 (Complex from Mus musculus)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Mus musculus)
- 4-[3-(4-fluoranyl-2~{h}-indazol-7-yl)-1,2,4-oxadiazol-5-yl]-1-(4-fluorophenyl)azepan-2-one (409 Da, Ligand)
- Cb2r-gi (Complex from Homo sapiens)
- Cannabinoid receptor 2 (35 kDa, Protein from Homo sapiens)
Qian Y, Ma Z, Xu Z, Duan Y, Xiong Y, Xia R, Zhu X, Zhang Z, Tian X, Yin H, Liu J, Song J, Lu Y, Zhang A, Guo C, Jin L, Kim WJ, Ke J, Xu F, Huang Z, He Y
Nat Commun (2024) 15 pp. 7644-7644 [ Pubmed: 39223191 DOI: doi:10.1038/s41467-024-52174-z ]
- Cholesterol hemisuccinate (486 Da, Ligand)
- Frizzled-4 (60 kDa, Protein from Homo sapiens)
- Gpcr complex (450 kDa, Complex from Homo sapiens)
- Segment polarity protein dishevelled homolog dvl-2 (79 kDa, Protein from Homo sapiens)
Fzd4/DEP complex (local refined)
Qian Y, Ma Z, Xu Z, Duan Y, Xiong Y, Xia R, Zhu X, Zhang Z, Tian X, Yin H, Liu J, Song J, Lu Y, Zhang A, Guo C, Jin L, Kim WJ, Ke J, Xu F, Huang Z, He Y
Nat Commun (2024) 15 pp. 7644-7644 [ Pubmed: 39223191 DOI: doi:10.1038/s41467-024-52174-z ]
- Frizzled-4 (60 kDa, Protein from Homo sapiens)
- Gpcr complex (450 kDa, Complex from Homo sapiens)
- Segment polarity protein dishevelled homolog dvl-2 (79 kDa, Protein from Homo sapiens)
Human TOM complex with whole Tom20
Resolution: 5.92 Å
EM Method: Single-particle
Fitted PDBs: 8xva
Q-score: 0.225
Su J, Tian X, Wang Z, Yang J, Sun S, Sui SF
Pnas Nexus (2024) 3 pp. pgae269-pgae269 [ DOI: doi:10.1093/pnasnexus/pgae269 Pubmed: 39071881 ]
- Mitochondrial import receptor subunit tom5 homolog (6 kDa, Protein from Homo sapiens)
- Mitochondrial import receptor subunit tom6 homolog (8 kDa, Protein from Homo sapiens)
- Mitochondrial import receptor subunit tom40 homolog (37 kDa, Protein from Homo sapiens)
- Tom complex with whole tom20 (168 MDa, Complex from Homo sapiens)
- Mitochondrial import receptor subunit tom22 homolog (15 kDa, Protein from Homo sapiens)
- Mitochondrial import receptor subunit tom20 homolog (16 kDa, Protein from Homo sapiens)
- Mitochondrial import receptor subunit tom7 homolog (6 kDa, Protein from Homo sapiens)
Su J, Tian X, Wang Z, Yang J, Sun S, Sui SF
Pnas Nexus (2024) 3 pp. pgae269-pgae269 [ DOI: doi:10.1093/pnasnexus/pgae269 Pubmed: 39071881 ]
- Tom complex with whole tom20 (168 MDa, Complex from Homo sapiens)
Su J, Tian X, Wang Z, Yang J, Sun S, Sui SF
Pnas Nexus (2024) 3 pp. pgae269-pgae269 [ DOI: doi:10.1093/pnasnexus/pgae269 Pubmed: 39071881 ]
- Tom complex with whole tom20 (168 MDa, Complex from Homo sapiens)