Structure of the HER4/NRG1b Homodimer Extracellular Domain
Resolution: 3.38 Å
EM Method: Single-particle
Fitted PDBs: 8u4i
Q-score: 0.388
Trenker R, Diwanji D, Bingham T, Verba KA, Jura N
eLife (2024) 12 [ DOI: doi:10.7554/eLife.92873 Pubmed: 38498590 ]
- Her4/nrg1b homodimer (Complex from Homo sapiens)
- Receptor tyrosine-protein kinase erbb-4 (68 kDa, Protein from Homo sapiens)
- Isoform 6 of pro-neuregulin-1, membrane-bound isoform (5 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Structure of the HER4/BTC Homodimer Extracellular Domain
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8u4j
Q-score: 0.359
Trenker R, Diwanji D, Bingham T, Verba KA, Jura N
eLife (2024) 12 [ DOI: doi:10.7554/eLife.92873 Pubmed: 38498590 ]
- Receptor tyrosine-protein kinase erbb-4 (68 kDa, Protein from Homo sapiens)
- Her4/btc homodimer (306 kDa, Complex from Homo sapiens)
- Betacellulin (5 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Structure of the HER2/HER4/BTC Heterodimer Extracellular Domain
Resolution: 4.27 Å
EM Method: Single-particle
Fitted PDBs: 8u4k
Q-score: 0.284
Trenker R, Diwanji D, Bingham T, Verba KA, Jura N
eLife (2024) 12 [ Pubmed: 38498590 DOI: doi:10.7554/eLife.92873 ]
- Isoform jm-a cyt-1 of receptor tyrosine-protein kinase erbb-4 (67 kDa, Protein from Homo sapiens)
- Ternary complex of her2/her4/btc (284 kDa, Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Receptor tyrosine-protein kinase erbb-2 (66 kDa, Protein from Homo sapiens)
- Betacellulin (5 kDa, Protein from Homo sapiens)
Structure of the HER2/HER4/NRG1b Heterodimer Extracellular Domain
Resolution: 3.31 Å
EM Method: Single-particle
Fitted PDBs: 8u4l
Q-score: 0.423
Trenker R, Diwanji D, Bingham T, Verba KA, Jura N
eLife (2024) 12 [ DOI: doi:10.7554/eLife.92873 Pubmed: 38498590 ]
- Receptor tyrosine-protein kinase erbb-2 (66 kDa, Protein from Homo sapiens)
- Isoform jm-a cyt-1 of receptor tyrosine-protein kinase erbb-4 (67 kDa, Protein from Homo sapiens)
- Ternary complex of her2/her4/nrg1b (Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Isoform 6 of pro-neuregulin-1, membrane-bound isoform (5 kDa, Protein from Homo sapiens)
Structure of the HER2/HER3/NRG1b Heterodimer Extracellular Domain bound to Trastuzumab Fab
Resolution: 3.45 Å
EM Method: Single-particle
Fitted PDBs: 7mn8
Q-score: 0.342
Diwanji D, Trenker R, Thaker TM, Wang F, Agard DA, Verba KA, Jura N
Nature (2021) 600 pp. 339-343 [ DOI: doi:10.1038/s41586-021-04084-z Pubmed: 34759323 ]
- Her2-s310f-mbp fusion (Complex from Homo sapiens)
- Nrg1b-bound her2/her3 heterodimer bound to the trastuzumab fab in the context of near full-length receptors (Complex)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Her3 (Complex from Homo sapiens)
- Neuregulin-1b isoform 6 (Complex from Homo sapiens)
- Trastuzumab fab heavy chain (25 kDa, Protein from Homo sapiens)
- Receptor tyrosine-protein kinase erbb-3 (117 kDa, Protein from Homo sapiens)
- Trastuzumab (herceptin) fab (Complex from Homo sapiens)
- Trastuzumab fab light chain (24 kDa, Protein from Homo sapiens)
- Isoform 6 of pro-neuregulin-1, membrane-bound isoform (9 kDa, Protein from Homo sapiens)
- Receptor tyrosine-protein kinase erbb-2,maltose/maltodextrin-binding periplasmic protein (160 kDa, Protein from Escherichia coli)
The HER2/HER3/NRG1b Heterodimer
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 7mn5
Q-score: 0.454
Diwanji D, Trenker R, Thaker TM, Wang F, Agard DA, Verba KA, Jura N
Nature (2021) 600 pp. 339-343 [ Pubmed: 34759323 DOI: doi:10.1038/s41586-021-04084-z ]
- Her3 (Complex from Homo sapiens)
- Her2/her3/nrg1b heterocomplex in the context of near full-length receptors (Complex)
- Isoform 6 of pro-neuregulin-1, membrane-bound isoform (9 kDa, Protein from Homo sapiens)
- Receptor tyrosine-protein kinase erbb-3 (117 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Her2-mbp fusion (Complex from Homo sapiens)
- Neuregulin-1 (Complex from Homo sapiens)
- Receptor tyrosine-protein kinase erbb-2,maltose/maltodextrin-binding periplasmic protein (160 kDa, Protein from Escherichia coli)
The HER2 S310F/HER3/NRG1b Heterodimer
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 7mn6
Q-score: 0.453
Diwanji D, Trenker R, Thaker TM, Wang F, Agard DA, Verba KA, Jura N
Nature (2021) 600 pp. 339-343 [ Pubmed: 34759323 DOI: doi:10.1038/s41586-021-04084-z ]
- Nrb1b-bound her2-s310f/her3 heterodimer in the context of near full-length receptors (Complex)
- Her3 (Complex from Homo sapiens)
- Her2-s310f, mbp fusion (Complex from Homo sapiens)
- Receptor tyrosine-protein kinase erbb-2,maltose/maltodextrin-binding periplasmic protein (160 kDa, Protein from Escherichia coli)
- Isoform 6 of pro-neuregulin-1, membrane-bound isoform (9 kDa, Protein from Homo sapiens)
- Receptor tyrosine-protein kinase erbb-3 (117 kDa, Protein from Homo sapiens)
- Neuregulin-1 (Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
SARS-CoV-2 Spike bound to Nb6 in closed conformation
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 7kkk
Q-score: 0.463
Schoof M, Faust B, Saunders RA, Sangwan S, Rezelj V, Hoppe N, Boone M, Billesbolle CB, Puchades C, Azumaya CM, Kratochvil HT, Zimanyi M, Deshpande I, Liang J, Dickinson S, Nguyen HC, Chio CM, Merz GE, Thompson MC, Diwanji D, Schaefer K, Anand AA, Dobzinski N, Zha BS, Simoneau CR, Leon K, White KM, Chio US, Gupta M, Jin M, Li F, Liu Y, Zhang K, Bulkley D, Sun M, Smith AM, Rizo AN, Moss F, Brilot AF, Pourmal S, Trenker R, Pospiech T, Gupta S, Barsi-Rhyne B, Belyy V, Barile-Hill AW, Nock S, Liu Y, Krogan NJ, Ralston CY, Swaney DL, Garcia-Sastre A, Ott M, Vignuzzi M, QCRG Structural Biology Consortium, Walter P, Manglik A
Science (2020) 370 pp. 1473-1479 [ Pubmed: 33154106 DOI: doi:10.1126/science.abe3255 ]
- Spike glycoprotein (142 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Synthetic nanobody nb6 (Complex from synthetic construct)
- Spike s2p glycoprotein (Complex from Severe acute respiratory syndrome coronavirus 2)
- Complex of spike s2p glycoprotein with synthetic nanobody nb6 (Complex)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Synthetic nanobody nb6 (12 kDa, Protein from synthetic construct)
SARS-CoV-2 Spike bound to Nb6 in open conformation
Schoof M, Faust B, Saunders RA, Sangwan S, Rezelj V, Hoppe N, Boone M, Billesbolle CB, Puchades C, Azumaya CM, Kratochvil HT, Zimanyi M, Deshpande I, Liang J, Dickinson S, Nguyen HC, Chio CM, Merz GE, Thompson MC, Diwanji D, Schaefer K, Anand AA, Dobzinski N, Zha BS, Simoneau CR, Leon K, White KM, Chio US, Gupta M, Jin M, Li F, Liu Y, Zhang K, Bulkley D, Sun M, Smith AM, Rizo AN, Moss F, Brilot AF, Pourmal S, Trenker R, Pospiech T, Gupta S, Barsi-Rhyne B, Belyy V, Barile-Hill AW, Nock S, Liu Y, Krogan NJ, Ralston CY, Swaney DL, Garcia-Sastre A, Ott M, Vignuzzi M, QCRG Structural Biology Consortium, Walter P, Manglik A
Science (2020) 370 pp. 1473-1479 [ Pubmed: 33154106 DOI: doi:10.1126/science.abe3255 ]
SARS-CoV-2 Spike bound to Nb11 in closed conformation
Schoof M, Faust B, Saunders RA, Sangwan S, Rezelj V, Hoppe N, Boone M, Billesbolle CB, Puchades C, Azumaya CM, Kratochvil HT, Zimanyi M, Deshpande I, Liang J, Dickinson S, Nguyen HC, Chio CM, Merz GE, Thompson MC, Diwanji D, Schaefer K, Anand AA, Dobzinski N, Zha BS, Simoneau CR, Leon K, White KM, Chio US, Gupta M, Jin M, Li F, Liu Y, Zhang K, Bulkley D, Sun M, Smith AM, Rizo AN, Moss F, Brilot AF, Pourmal S, Trenker R, Pospiech T, Gupta S, Barsi-Rhyne B, Belyy V, Barile-Hill AW, Nock S, Liu Y, Krogan NJ, Ralston CY, Swaney DL, Garcia-Sastre A, Ott M, Vignuzzi M, QCRG Structural Biology Consortium, Walter P, Manglik A
Science (2020) 370 pp. 1473-1479 [ Pubmed: 33154106 DOI: doi:10.1126/science.abe3255 ]