Structure of signaling thrombopoietin-MPL receptor complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8g04
Q-score: 0.44
Tsutsumi N, Masoumi Z, James SC, Tucker JA, Winkelmann H, Grey W, Picton LK, Moss L, Wilson SC, Caveney NA, Jude KM, Gati C, Piehler J, Hitchcock IS, Garcia KC
Cell (2023) 186 pp. 4189-4203.e22 [ Pubmed: 37633268 DOI: doi:10.1016/j.cell.2023.07.037 ]
- Thrombopoietin receptor (71 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Thrombopoietin (18 kDa, Protein from Homo sapiens)
- Thrombopoietin signaling complex with two thrombopoietin receptor chains (160 kDa, Complex from Homo sapiens)
- Alpha-d-mannopyranose (180 Da, Ligand)
CryoEM micrographs of guanylyl cyclase receptor–Hsp90 complex
GC-C-Hsp90-Cdc37 regulatory complex
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 8fx4
Q-score: 0.329
Caveney NA, Tsutsumi N, Garcia KC
eLife (2023) 12 [ DOI: doi:10.7554/eLife.86784 Pubmed: 37535399 ]
- Hsp90 co-chaperone cdc37 (44 kDa, Protein from Cricetulus griseus)
- Gc-c-hsp90-cdc37 regulatory complex (Complex)
- Guanylyl cyclase c (85 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Heat shock protein hsp 90-beta (83 kDa, Protein from Cricetulus griseus)
Micrographs of the IL-27 quaternary receptor signaling complex
Caveney NA, Glassman CR, Jude KM, Tsutsumi N, Garcia KC
eLife (2022) 11 [ DOI: 10.7554/elife.78463 Pubmed: 35579417 ]
Image sets:
Extracellular architecture of an engineered canonical Wnt signaling ternary complex
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8ctg
Q-score: 0.109
Tsutsumi N, Hwang S, Waghray D, Hansen S, Jude KM, Wang N, Miao Y, Glassman CR, Caveney NA, Janda CY, Hannoush RN, Garcia KC
PNAS (2023) 120 pp. e2218238120-e2218238120 [ Pubmed: 36893265 DOI: doi:10.1073/pnas.2218238120 ]
- Haxwnt8-mfzd8crd-hlrp6e1e2 (130 kDa, Complex)
- Low-density lipoprotein receptor-related protein 6 (73 kDa, Protein from Homo sapiens)
- Palmitoleic acid (254 Da, Ligand)
- Frizzled-8 (15 kDa, Protein from Mus musculus)
- Low-density lipoprotein receptor-related protein 6 (Complex from Homo sapiens)
- Protein wnt-8 (36 kDa, Protein from Xenopus laevis)
- Protein wnt-8 (Complex from Xenopus laevis)
- Frizzled-8 (Complex from Mus musculus)
Structure of Janus Kinase (JAK) dimer complexed with cytokine receptor intracellular domain
Resolution: 5.5 Å
EM Method: Single-particle
Fitted PDBs: 8ewy
Q-score: 0.296
Caveney NA, Saxton RA, Waghray D, Glassman CR, Tsutsumi N, Hubbard SR, Garcia KC
Cell Rep (2023) 42 pp. 112201-112201 [ Pubmed: 36867534 DOI: doi:10.1016/j.celrep.2023.112201 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Tyrosine-protein kinase (136 kDa, Protein from Mus musculus)
- Structure of janus kinase (jak) dimer complexed with cytokine receptor intracellular domain (Complex from Mus musculus)
- Adenosine (267 Da, Ligand)
- Interferon lambda receptor 1 (9 kDa, Protein from Mus musculus)
Organizing Structural Principles of the Interleukin-17 Ligand-Receptor Axis
Wilson SC, Caveney NA, Yen M, Pollmann C, Xiang X, Jude KM, Hafer M, Tsutsumi N, Piehler J, Garcia KC
Nature (2022) 609 pp. 622-629 [ DOI: 10.1038/s41586-022-05116-y Pubmed: 35863378 ]
Image sets:
- Unaligned micrographs of IL-25 binary complex at 40 degree tilt.
- Unaligned micrographs of IL-25 ternary complex at 0 degree tilt.
- Unaligned micrographs of IL-25 binary complex at 0 degree tilt.
- Unaligned micrographs of IL-25 ternary complex at 35 degree tilt.
Structure of the homodimeric IL-25-IL-17RB binary complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7uwj
Q-score: 0.316
Wilson SC, Caveney NA, Yen M, Pollmann C, Xiang X, Jude KM, Hafer M, Tsutsumi N, Piehler J, Garcia KC
Nature (2022) 609 pp. 622-629 [ Pubmed: 35863378 DOI: doi:10.1038/s41586-022-05116-y ]
- Homodimeric il-25-il-17rb binary complex (Complex from Homo sapiens)
- Interleukin-17 receptor b (28 kDa, Protein from Homo sapiens)
- Interleukin-25 (21 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Structure of the higher-order IL-25-IL-17RB complex
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 7uwk
Q-score: 0.165
Wilson SC, Caveney NA, Yen M, Pollmann C, Xiang X, Jude KM, Hafer M, Tsutsumi N, Piehler J, Garcia KC
Nature (2022) 609 pp. 622-629 [ Pubmed: 35863378 DOI: doi:10.1038/s41586-022-05116-y ]
- Higher-order il-25-il-17rb complex (Complex from Homo sapiens)
- Interleukin-17 receptor b (33 kDa, Protein from Homo sapiens)
- Interleukin-25 (21 kDa, Protein from Homo sapiens)
Structure of the IL-25-IL-17RB-IL-17RA ternary complex
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7uwl
Q-score: 0.269
Wilson SC, Caveney NA, Yen M, Pollmann C, Xiang X, Jude KM, Hafer M, Tsutsumi N, Piehler J, Garcia KC
Nature (2022) 609 pp. 622-629 [ Pubmed: 35863378 DOI: doi:10.1038/s41586-022-05116-y ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Ternary il-25-il-17rb-il-17ra complex (Complex from Homo sapiens)
- Interleukin-17 receptor a (36 kDa, Protein from Homo sapiens)
- Interleukin-17 receptor b (33 kDa, Protein from Homo sapiens)
- Interleukin-25 (21 kDa, Protein from Homo sapiens)