Structure of yeast replisome associated with FACT and histone hexamer, Composite map
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8xgc
Q-score: 0.407
Li N, Gao Y, Zhang Y, Yu D, Lin J, Feng J, Li J, Xu Z, Zhang Y, Dang S, Zhou K, Liu Y, Li XD, Tye BK, Li Q, Gao N, Zhai Y
Nature (2024) 627 pp. 890-897 [ Pubmed: 38448592 DOI: doi:10.1038/s41586-024-07152-2 ]
- Endogenous replisomes (Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Histone h2a.1 (14 kDa, Protein from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Dna (51-mer) (15 kDa, DNA from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Dna (39-mer) (11 kDa, DNA from Saccharomyces cerevisiae)
- Dna replication complex gins protein sld5 (33 kDa, Protein from Saccharomyces cerevisiae)
- Mediator of replication checkpoint protein 1 (124 kDa, Protein from Saccharomyces cerevisiae)
- Chromosome segregation in meiosis protein 3 (36 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase epsilon subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Histone h2b.2 (14 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf3 (21 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase alpha-binding protein (104 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase epsilon catalytic subunit a (255 kDa, Protein from Saccharomyces cerevisiae)
- Fact complex subunit spt16 (118 kDa, Protein from Saccharomyces cerevisiae)
- Fact complex subunit pob3 (63 kDa, Protein from Saccharomyces cerevisiae)
- Cell division control protein 45 (74 kDa, Protein from Saccharomyces cerevisiae)
- Histone h4 (11 kDa, Protein from Saccharomyces cerevisiae)
- Topoisomerase 1-associated factor 1 (141 kDa, Protein from Saccharomyces cerevisiae)
- Histone h3 (15 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf2 (29 kDa, Protein from Saccharomyces cerevisiae)
Human MCM double hexamer bound to natural DNA duplex (polyAT/polyTA)
Resolution: 2.59 Å
EM Method: Single-particle
Fitted PDBs: 7w1y
Q-score: 0.595
Li J, Dong J, Wang W, Yu D, Fan X, Hui YC, Lee CSK, Lam WH, Alary N, Yang Y, Zhang Y, Zhao Q, Chen CL, Tye BK, Dang S, Zhai Y
Cell (2023) 186 pp. 98-111.e21 [ DOI: doi:10.1016/j.cell.2022.12.008 Pubmed: 36608662 ]
- Dna replication licensing factor mcm7 (81 kDa, Protein from Homo sapiens)
- Dna (49-mer) (15 kDa, DNA from Homo sapiens)
- Dna replication licensing factor mcm5 (82 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm 2/3/4/5/6/7 (Complex from Homo sapiens)
- Human mini-chromosome maintenance complex (1 MDa, Complex from Homo sapiens)
- Dna replication licensing factor mcm2 (102 kDa, Protein from Homo sapiens)
- Dna (Complex)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Isoform 2 of dna replication licensing factor mcm3 (96 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna replication licensing factor mcm4 (96 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm6 (93 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna (49-mer) (14 kDa, DNA from Homo sapiens)
Cryo-EM structure of MCM double hexamer with structured Mcm4-NSD
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7v3u
Q-score: 0.514
Cheng J, Li N, Huo Y, Dang S, Tye BK, Gao N, Zhai Y
Nat Commun (2022) 13 pp. 1396-1396 [ DOI: doi:10.1038/s41467-022-29070-5 Pubmed: 35296675 ]
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae S288C)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae S288C)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Mcm double hexamer (dh) (Complex from Saccharomyces cerevisiae S288c)
- Zinc ion (65 Da, Ligand)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae S288C)
Cryo-EM structure of MCM double hexamer bound with DDK in State I
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7v3v
Q-score: 0.477
Cheng J, Li N, Huo Y, Dang S, Tye BK, Gao N, Zhai Y
Nat Commun (2022) 13 pp. 1396-1396 [ DOI: doi:10.1038/s41467-022-29070-5 Pubmed: 35296675 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae S288C)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dh-ddk (state i) (Complex from Saccharomyces cerevisiae S288c)
- Ddk kinase regulatory subunit dbf4 (80 kDa, Protein from Saccharomyces cerevisiae S288C)
- Cell division control protein 7 (58 kDa, Protein from Saccharomyces cerevisiae S288C)
- Zinc ion (65 Da, Ligand)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae S288C)