Consensus olfactory receptor consOR51 in complex with mini-Gs trimeric protein
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8uxv
Q-score: 0.544
de March CA, Ma N, Billesbolle CB, Tewari J, Llinas Del Torrent C, van der Velden WJC, Ojiro I, Takayama I, Faust B, Li L, Vaidehi N, Manglik A, Matsunami H
Nature (2024) 635 pp. 499-508 [ Pubmed: 39478229 DOI: doi:10.1038/s41586-024-08126-0 ]
- Consor51 (36 kDa, Protein from synthetic construct)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Minigs399 (30 kDa, Protein from Homo sapiens)
- Consor51-minigs complex bound to nb35 (100 kDa, Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Consensus olfactory receptor consOR1 bound to L-menthol and in complex with mini-Gs trimeric protein
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8uxy
Q-score: 0.402
de March CA, Ma N, Billesbolle CB, Tewari J, Llinas Del Torrent C, van der Velden WJC, Ojiro I, Takayama I, Faust B, Li L, Vaidehi N, Manglik A, Matsunami H
Nature (2024) 635 pp. 499-508 [ Pubmed: 39478229 DOI: doi:10.1038/s41586-024-08126-0 ]
- Gnas complex locus (30 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Consor51-minigs complex bound to nb35 (100 kDa, Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- (1r,2s,5r)-5-methyl-2-(propan-2-yl)cyclohexan-1-ol (156 Da, Ligand)
- Consor1 (35 kDa, Protein from synthetic construct)
Consensus olfactory receptor consOR2 bound to S-carvone and in complex with mini-Gs trimeric protein
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8uy0
Q-score: 0.489
de March CA, Ma N, Billesbolle CB, Tewari J, Llinas Del Torrent C, van der Velden WJC, Ojiro I, Takayama I, Faust B, Li L, Vaidehi N, Manglik A, Matsunami H
Nature (2024) 635 pp. 499-508 [ Pubmed: 39478229 DOI: doi:10.1038/s41586-024-08126-0 ]
- Consor2 (35 kDa, Protein from synthetic construct)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Minigs399 (30 kDa, Protein from Homo sapiens)
- (5s)-2-methyl-5-(prop-1-en-2-yl)cyclohex-2-en-1-one (150 Da, Ligand)
- Consor2-minigs complex bound to nb35 (100 kDa, Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8uyq
Q-score: 0.262
de March CA, Ma N, Billesbolle CB, Tewari J, Llinas Del Torrent C, van der Velden WJC, Ojiro I, Takayama I, Faust B, Li L, Vaidehi N, Manglik A, Matsunami H
Nature (2024) 635 pp. 499-508 [ Pubmed: 39478229 DOI: doi:10.1038/s41586-024-08126-0 ]
- Consor4-minigs complex bound to nb35 (100 kDa, Complex from synthetic construct)
- Gnas complex locus (30 kDa, Protein from Homo sapiens)
- (2r)-2-methyl-1,3-thiazolidine (103 Da, Ligand)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Consor4 (36 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
CryoEM structure of D2 dopamine receptor in complex with GoA KE mutant, scFv16, and dopamine
Knight KM, Krumm BE, Kapolka NJ, Ludlam WG, Cui M, Mani S, Prytkova I, Obarow EG, Lefevre TJ, Wei W, Ma N, Huang XP, Fay JF, Vaidehi N, Smrcka AV, Slesinger PA, Logothetis DE, Martemyanov KA, Roth BL, Dohlman HG
Nat Commun (2024) 15 pp. 6643-6643 [ Pubmed: 39103320 DOI: doi:10.1038/s41467-024-50964-z ]
- D(2) dopamine receptor (50 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- L-dopamine (153 Da, Ligand)
- Human drd2 in complex with heterotrimeric g protein goa (k46e), scfv16, and dopamine (120 kDa, Complex from Homo sapiens)
- Scfv16 (28 kDa, Protein from Escherichia coli)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
CryoEM structure of D2 dopamine receptor in complex with GoA KE mutant and dopamine
Knight KM, Krumm BE, Kapolka NJ, Ludlam WG, Cui M, Mani S, Prytkova I, Obarow EG, Lefevre TJ, Wei W, Ma N, Huang XP, Fay JF, Vaidehi N, Smrcka AV, Slesinger PA, Logothetis DE, Martemyanov KA, Roth BL, Dohlman HG
Nat Commun (2024) 15 pp. 6643-6643 [ Pubmed: 39103320 DOI: doi:10.1038/s41467-024-50964-z ]
- D(2) dopamine receptor (50 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- L-dopamine (153 Da, Ligand)
- Human drd2 in complex with heterotrimeric g protein goa (k46e) and dopamine (120 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 8pm2
Q-score: 0.496
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Verprintsev DB, Vaidehi N, Weyand SN, Tate CG
bioRxiv (2023) [ DOI: doi:10.1101/2023.07.07.547762 Pubmed: 37461561 ]
- ~{n},~{n}-dimethylcyclohexanamine (127 Da, Ligand)
- Trace amine-associated receptor 7f (41 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- A complex of mouse trace-amine associated receptor 7f solubilized in lmng/chs bound to n,n-dimethylcyclohexylamine and coupled to: engineered guanine nucleotide-binding protein g(s) subunit alpha isoform short + guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 + guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 + nanobody 35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Nanobody 35 (16 kDa, Protein from Lama glama)
Human olfactory receptor OR51E2 bound to propionate in complex with miniGs399
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8f76
Q-score: 0.52
Billesbolle CB, de March CA, van der Velden WJC, Ma N, Tewari J, Del Torrent CL, Li L, Faust B, Vaidehi N, Matsunami H, Manglik A
Nature (2023) 615 pp. 742-749 [ DOI: doi:10.1038/s41586-023-05798-y Pubmed: 36922591 ]
- Or51e2-gs complex (100 kDa, Complex from Homo sapiens)
- Propanoic acid (74 Da, Ligand)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Olfactory receptor 51e2 (36 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
Structure of the GPCR dimer Ste2 bound to an antagonist
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7qa8
Q-score: 0.538
Velazhahan V, Ma N, Vaidehi N, Tate CG
Nature (2022) 603 pp. 743-748 [ DOI: doi:10.1038/s41586-022-04498-3 Pubmed: 35296853 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Water (18 Da, Ligand)
- Gpcr dimer ste2 bound to an antagonist (Complex from Saccharomyces cerevisiae)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Pheromone alpha factor receptor (47 kDa, Protein from Saccharomyces cerevisiae)
- His-ala-leu-gln-leu-lys-pro-gly-gln-pro-nle-tyr (1 kDa, Protein from Saccharomyces cerevisiae)
Structure of the ligand-free GPCR dimer Ste2
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7qb9
Q-score: 0.501
Velazhahan V, Ma N, Vaidehi N, Tate CG
Nature (2022) 603 pp. 743-748 [ DOI: doi:10.1038/s41586-022-04498-3 Pubmed: 35296853 ]
- Cholesterol hemisuccinate (486 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Pheromone alpha factor receptor (47 kDa, Protein from Saccharomyces cerevisiae)
- Structure of the ligand-free gpcr dimer ste2 (Complex from Saccharomyces cerevisiae)