Single particle reconstruction of the human LINE-1 ORF2p without substrate (apo)
Baldwin ET, van Eeuwen T, Hoyos D, Zalevsky A, Tchesnokov EP, Sánchez R, Miller BD, Di Stefano LH, Ruiz FX, Hancock M, Işik E, Mendez-Dorantes C, Walpole T, Nichols C, Wan P, Riento K, Halls-Kass R, Augustin M, Lammens A, Jestel A, Upla P, Xibinaku K, Congreve S, Hennink M, Rogala KB, Schneider AM, Fairman JE, Christensen SM, Desrosiers B, Bisacchi GS, Saunders OL, Hafeez N, Miao W, Kapeller R, Zaller DM, Sali A, Weichenrieder O, Burns KH, Götte M, Rout MP, Arnold E, Greenbaum BD, Romero DL, LaCava J, Taylor MS
Nature (2023) 626 pp. 194-206 [ DOI: 10.1038/s41586-023-06947-z Pubmed: 38096902 ]
Image sets:
Raw data used to generate the structure of cortactin bound to the Arp2/3 complex
Fregoso FE, Boczkowska M, Rebowski G, Carman PJ, van Eeuwen T, Dominguez R
Nat Commun (2023) 14 [ DOI: 10.1038/s41467-023-42229-y Pubmed: 37898612 ]
Image sets:
Single particle reconstruction of the human LINE-1 ORF2p without substrate (apo)
Baldwin ET, van Eeuwen T, Hoyos D, Zalevsky A, Tchesnokov EP, Sanchez R, Miller BD, Di Stefano LH, Ruiz FX, Hancock M, Isik E, Mendez-Dorantes C, Walpole T, Nichols C, Wan P, Riento K, Halls-Kass R, Augustin M, Lammens A, Jestel A, Upla P, Xibinaku K, Congreve S, Hennink M, Rogala KB, Schneider AM, Fairman JE, Christensen SM, Desrosiers B, Bisacchi GS, Saunders OL, Hafeez N, Miao W, Kapeller R, Zaller DM, Sali A, Weichenrieder O, Burns KH, Gotte M, Rout MP, Arnold E, Greenbaum BD, Romero DL, LaCava J, Taylor MS
Nature (2024) 626 pp. 194-206 [ DOI: doi:10.1038/s41586-023-06947-z Pubmed: 38096902 ]
Structure of LINE-1 ORF2p with template:primer hybrid
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8sxt
Q-score: 0.339
Baldwin ET, van Eeuwen T, Hoyos D, Zalevsky A, Tchesnokov EP, Sanchez R, Miller BD, Di Stefano LH, Ruiz FX, Hancock M, Isik E, Mendez-Dorantes C, Walpole T, Nichols C, Wan P, Riento K, Halls-Kass R, Augustin M, Lammens A, Jestel A, Upla P, Xibinaku K, Congreve S, Hennink M, Rogala KB, Schneider AM, Fairman JE, Christensen SM, Desrosiers B, Bisacchi GS, Saunders OL, Hafeez N, Miao W, Kapeller R, Zaller DM, Sali A, Weichenrieder O, Burns KH, Gotte M, Rout MP, Arnold E, Greenbaum BD, Romero DL, LaCava J, Taylor MS
Nature (2024) 626 pp. 194-206 [ DOI: doi:10.1038/s41586-023-06947-z Pubmed: 38096902 ]
- Thymidine-5'-triphosphate (482 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Long interspersed nuclear element (line)-1 orf2 protein (Complex from Homo sapiens)
- Dna primer (3 kDa, DNA from synthetic construct)
- Rna template (5 kDa, RNA from synthetic construct)
- Line-1 retrotransposable element orf2 protein (149 kDa, Protein from Homo sapiens)
Structure of LINE-1 ORF2p with an oligo(A) template
Resolution: 3.66 Å
EM Method: Single-particle
Fitted PDBs: 8sxu
Q-score: 0.185
Baldwin ET, van Eeuwen T, Hoyos D, Zalevsky A, Tchesnokov EP, Sanchez R, Miller BD, Di Stefano LH, Ruiz FX, Hancock M, Isik E, Mendez-Dorantes C, Walpole T, Nichols C, Wan P, Riento K, Halls-Kass R, Augustin M, Lammens A, Jestel A, Upla P, Xibinaku K, Congreve S, Hennink M, Rogala KB, Schneider AM, Fairman JE, Christensen SM, Desrosiers B, Bisacchi GS, Saunders OL, Hafeez N, Miao W, Kapeller R, Zaller DM, Sali A, Weichenrieder O, Burns KH, Gotte M, Rout MP, Arnold E, Greenbaum BD, Romero DL, LaCava J, Taylor MS
Nature (2024) 626 pp. 194-206 [ DOI: doi:10.1038/s41586-023-06947-z Pubmed: 38096902 ]
- Long interspersed nuclear element (line)-1 orf2 protein (Complex from Homo sapiens)
- Rna (25-mer) (8 kDa, RNA from synthetic construct)
- Line-1 retrotransposable element orf2 protein (149 kDa, Protein from Homo sapiens)
Cryo-EM structure of Cortactin-bound to Arp2/3 complex
Resolution: 2.89 Å
EM Method: Single-particle
Fitted PDBs: 8tah
Q-score: 0.514
Fregoso FE, Boczkowska M, Rebowski G, Carman PJ, van Eeuwen T, Dominguez R
Nat Commun (2023) 14 pp. 6894-6894 [ DOI: doi:10.1038/s41467-023-42229-y Pubmed: 37898612 ]
- Cortactin nta bound to arp2/3 complex (223 kDa, Complex from Bos taurus)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Actin-related protein 3 (47 kDa, Protein from Bos taurus)
- Actin-related protein 2/3 complex subunit 3 (20 kDa, Protein from Bos taurus)
- Actin-related protein 2/3 complex subunit 4 (19 kDa, Protein from Bos taurus)
- Src substrate cortactin (8 kDa, Protein from Mus musculus)
- Actin-related protein 2/3 complex subunit 5 (16 kDa, Protein from Bos taurus)
- Actin-related protein 2/3 complex subunit 2 (34 kDa, Protein from Bos taurus)
- Actin-related protein 2 (44 kDa, Protein from Bos taurus)
- Actin-related protein 2/3 complex subunit 1a (41 kDa, Protein from Bos taurus)
Cryo-EM Structure of a Transition State of Arp2/3 Complex Activation
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7t5q
Q-score: 0.385
van Eeuwen T, Boczkowska M, Rebowski G, Carman PJ, Fregoso FE, Dominguez R
PNAS (2023) 120 pp. e2306165120-e2306165120 [ DOI: doi:10.1073/pnas.2306165120 Pubmed: 37549294 ]
- Magnesium ion (24 Da, Ligand)
- Actin skeletal muscle (Complex from Oryctolagus cuniculus)
- F-actin-capping protein subunit beta/actin nucleation-promoting factor wasl chimera (37 kDa, Protein from Mus musculus)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- F-actin-capping protein subunit alpha-1/actin nucleation-promoting factor wasl chimera (39 kDa, Protein from Mus musculus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin-related protein 3 (47 kDa, Protein from Bos taurus)
- Bovine arp2/3 complex and actin, capz (248 kDa, Complex)
- Actin-related protein 2/3 complex subunit 3 (20 kDa, Protein from Bos taurus)
- Actin-related protein 2/3 complex subunit 4 (19 kDa, Protein from Bos taurus)
- Bovine arp2/3 (Complex from Bos taurus)
- Actin-related protein 2/3 complex subunit 5 (16 kDa, Protein from Bos taurus)
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Actin-related protein 2/3 complex subunit 2 (34 kDa, Protein from Bos taurus)
- Actin-related protein 2 (44 kDa, Protein from Bos taurus)
- Actin-related protein 2/3 complex subunit 1a (41 kDa, Protein from Bos taurus)
- F-actin-capping protein (Complex from Homo sapiens)
Resolution: 3.73 Å
EM Method: Single-particle
Fitted PDBs: 8eax
Q-score: 0.339
Faylo JL, van Eeuwen T, Gupta K, Murakami K, Christianson DW
Biochemistry (2022) 61 pp. 2417-2430 [ Pubmed: 36227241 DOI: doi:10.1021/acs.biochem.2c00509 ]
Faylo JL, van Eeuwen T, Gupta K, Murakami K, Christianson DW
Biochemistry (2022) 61 pp. 2417-2430 [ Pubmed: 36227241 DOI: doi:10.1021/acs.biochem.2c00509 ]
Symmetry-expanded map of PaFS prenyltransferase core with single interacting cyclase domain class A
Faylo JL, van Eeuwen T, Gupta K, Murakami K, Christianson DW
Biochemistry (2022) 61 pp. 2417-2430 [ Pubmed: 36227241 DOI: doi:10.1021/acs.biochem.2c00509 ]