Structure of Integrator-PP2A bound to a paused RNA polymerase II-DSIF-NELF-nucleosome complex
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 8rbx
Q-score: 0.037
Fianu I, Ochmann M, Walshe JL, Dybkov O, Cruz JN, Urlaub H, Cramer P
Nature (2024) 629 pp. 219-227 [ DOI: doi:10.1038/s41586-024-07269-4 Pubmed: 38570683 ]
- Serine/threonine-protein phosphatase 2a 65 kda regulatory subunit a alpha isoform (65 kDa, Protein from Homo sapiens)
- Integrator complex subunit 5 (108 kDa, Protein from Homo sapiens)
- Dsif (Complex from Trichoplusia ni)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from Sus scrofa)
- Negative elongation factor c/d (65 kDa, Protein from Homo sapiens)
- Template dna (56 kDa, DNA from synthetic construct)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Integrator complex subunit 14 (57 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Sus scrofa)
- Transcription elongation factor spt5 (121 kDa, Protein from Homo sapiens)
- Integrator complex subunit 11 (67 kDa, Protein from Homo sapiens)
- Integrator complex subunit 10 (82 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase subunit (217 kDa, Protein from Sus scrofa)
- Rna polymerase ii subunit k (7 kDa, Protein from Sus scrofa)
- Integrator complex subunit 4 (108 kDa, Protein from Homo sapiens)
- Integrator complex subunit 15 (50 kDa, Protein from Homo sapiens)
- Integrator-pp2a complex and nelf (Complex from Homo sapiens)
- Histone h2a type 1 (14 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit e (24 kDa, Protein from Sus scrofa)
- Integrator complex subunit 1 (244 kDa, Protein from Homo sapiens)
- Negative elongation factor a (57 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Sus scrofa)
- Manganese (ii) ion (54 Da, Ligand)
- Dna-directed rna polymerase ii subunit rpb11-a (13 kDa, Protein from Sus scrofa)
- Rna (5 kDa, RNA from synthetic construct)
- Serine/threonine-protein phosphatase 2a catalytic subunit alpha isoform (35 kDa, Protein from Homo sapiens)
- Pre-termination complex (1 MDa, Complex)
- Histone h2b type 1-j (13 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Integrator complex subunit 6 (100 kDa, Protein from Homo sapiens)
- Unk-unk-unk-unk-unk-unk-unk-unk (698 Da, Protein from Sus scrofa)
- Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Sus scrofa)
- Dna-directed rna polymerase ii (Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Sus scrofa)
- Rna polymerase ii subunit d (16 kDa, Protein from Sus scrofa)
- Dna-directed rna polymerase subunit beta (134 kDa, Protein from Sus scrofa)
- Integrator complex subunit 7 (107 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Sus scrofa)
- Dss1 (3 kDa, Protein from Trichoplusia ni)
- Histone h3.3c (15 kDa, Protein from Homo sapiens)
- Unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk (1 kDa, Protein from Homo sapiens)
- Integrator complex subunit 8 (113 kDa, Protein from Homo sapiens)
- Rna and dna (Complex from Sus scrofa)
- Non-template dna (45 kDa, DNA from synthetic construct)
- Integrator complex subunit 13 (80 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Nucleosome (Complex from Homo sapiens)
- Integrator complex subunit 9 (73 kDa, Protein from Homo sapiens)
- Integrator complex subunit 2 (134 kDa, Protein from Homo sapiens)
- Nelf-b,negative elongation factor b,negative elongation factor b,negative elongation factor b,negative elongation factor b,negative elongation factor b (68 kDa, Protein from Homo sapiens)
Structure of Integrator-PP2A-SOSS-CTD post-termination complex
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8rbz
Q-score: 0.293
Fianu I, Ochmann M, Walshe JL, Dybkov O, Cruz JN, Urlaub H, Cramer P
Nature (2024) 629 pp. 219-227 [ DOI: doi:10.1038/s41586-024-07269-4 Pubmed: 38570683 ]
- Serine/threonine-protein phosphatase 2a 65 kda regulatory subunit a alpha isoform (65 kDa, Protein from Homo sapiens)
- Integrator complex subunit 5 (108 kDa, Protein from Homo sapiens)
- Integrator complex subunit 3 (118 kDa, Protein from Homo sapiens)
- Integrator-pp2a complex and nabp2 and inip (Complex from Homo sapiens)
- Integrator complex subunit 14 (57 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase subunit (1 kDa, Protein from Sus scrofa)
- Integrator complex subunit 11 (67 kDa, Protein from Homo sapiens)
- Integrator complex subunit 10 (82 kDa, Protein from Homo sapiens)
- Dss1 (Complex from Trichoplusia ni)
- Integrator complex subunit 4 (108 kDa, Protein from Homo sapiens)
- Soss complex subunit c (11 kDa, Protein from Homo sapiens)
- Integrator complex subunit 15 (50 kDa, Protein from Homo sapiens)
- Integrator complex subunit 1 (244 kDa, Protein from Homo sapiens)
- Manganese (ii) ion (54 Da, Ligand)
- Serine/threonine-protein phosphatase 2a catalytic subunit alpha isoform (35 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Integrator complex subunit 6 (100 kDa, Protein from Homo sapiens)
- Post-termination complex (1 MDa, Complex)
- Rna polymerase ii c-terminal domain peptides (Complex from Sus scrofa)
- Unk-unk-unk-unk-unk-unk-unk-unk-unk (783 Da, Protein from Sus scrofa)
- Integrator complex subunit 7 (107 kDa, Protein from Homo sapiens)
- Soss complex subunit b1 (22 kDa, Protein from Homo sapiens)
- Integrator complex subunit 8 (113 kDa, Protein from Homo sapiens)
- Dss1 (3 kDa, Protein from Trichoplusia ni)
- Integrator complex subunit 13 (80 kDa, Protein from Homo sapiens)
- Integrator complex subunit 9 (73 kDa, Protein from Homo sapiens)
- Integrator complex subunit 2 (134 kDa, Protein from Homo sapiens)
Structure of Integrator-PP2A complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8rc4
Q-score: 0.036
Fianu I, Ochmann M, Walshe JL, Dybkov O, Cruz JN, Urlaub H, Cramer P
Nature (2024) 629 pp. 219-227 [ DOI: doi:10.1038/s41586-024-07269-4 Pubmed: 38570683 ]
- Dss1 (3 kDa, Protein from Trichoplusia ni)
- Serine/threonine-protein phosphatase 2a 65 kda regulatory subunit a alpha isoform (65 kDa, Protein from Homo sapiens)
- Integrator complex subunit 5 (108 kDa, Protein from Homo sapiens)
- Integrator-pp2a complex (Complex from Homo sapiens)
- Integrator complex subunit 14 (57 kDa, Protein from Homo sapiens)
- Integrator complex subunit 10 (82 kDa, Protein from Homo sapiens)
- Dss1 (Complex from Trichoplusia ni)
- Integrator complex subunit 4 (108 kDa, Protein from Homo sapiens)
- Integrator complex subunit 15 (50 kDa, Protein from Homo sapiens)
- Integrator complex subunit 1 (244 kDa, Protein from Homo sapiens)
- Integrator-pp2a complex (1 MDa, Complex)
- Manganese (ii) ion (54 Da, Ligand)
- Serine/threonine-protein phosphatase 2a catalytic subunit alpha isoform (35 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Integrator complex subunit 6 (101 kDa, Protein from Homo sapiens)
- Integrator complex subunit 7 (107 kDa, Protein from Homo sapiens)
- Integrator complex subunit 8 (113 kDa, Protein from Homo sapiens)
- Integrator complex subunit 13 (80 kDa, Protein from Homo sapiens)
- Integrator complex subunit 11 (67 kDa, Protein from Homo sapiens)
- Integrator complex subunit 9 (73 kDa, Protein from Homo sapiens)
- Integrator complex subunit 2 (134 kDa, Protein from Homo sapiens)
E. coli ATP synthase imaged in 10mM MgATP State1
Sobti M, Zeng YC, Walshe JL, Brown SHJ, Ishmukhametov R, Stewart AG
Commun Biol (2023) 6 pp. 26-26 [ Pubmed: 36631659 DOI: doi:10.1038/s42003-023-04414-z ]
- Atp synthase (Complex from Escherichia coli)
E. coli ATP synthase imaged in 10mM MgATP State1 "half-up
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8dbp
Q-score: 0.374
Sobti M, Zeng YC, Walshe JL, Brown SHJ, Ishmukhametov R, Stewart AG
Commun Biol (2023) 6 pp. 26-26 [ Pubmed: 36631659 DOI: doi:10.1038/s42003-023-04414-z ]
- Atp synthase epsilon chain (15 kDa, Protein from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Atp synthase subunit alpha (55 kDa, Protein from Escherichia coli)
- Atp synthase subunit c (8 kDa, Protein from Escherichia coli)
- Atp synthase subunit a (30 kDa, Protein from Escherichia coli)
- Atp synthase subunit beta (51 kDa, Protein from Escherichia coli)
- Atp synthase subunit delta (19 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Atp synthase gamma chain (31 kDa, Protein from Escherichia coli)
- Atp synthase subunit b (17 kDa, Protein from Escherichia coli)
- Atp synthase (Complex from Escherichia coli)
E.coli ATP synthase imaged in 10mM MgATP State1 "half-up" Fo classified
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 8dbq
Q-score: 0.307
Sobti M, Zeng YC, Walshe JL, Brown SHJ, Ishmukhametov R, Stewart AG
Commun Biol (2023) 6 pp. 26-26 [ Pubmed: 36631659 DOI: doi:10.1038/s42003-023-04414-z ]
- Atp synthase (Complex from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Atp synthase subunit alpha (54 kDa, Protein from Escherichia coli)
- Atp synthase subunit delta (18 kDa, Protein from Escherichia coli)
- Atp synthase subunit c (7 kDa, Protein from Escherichia coli)
- Atp synthase subunit alpha (55 kDa, Protein from Escherichia coli)
- Atp synthase subunit b (17 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Atp synthase subunit a (29 kDa, Protein from Escherichia coli)
- Atp synthase subunit beta (50 kDa, Protein from Escherichia coli)
- Atp synthase gamma chain (31 kDa, Protein from Escherichia coli)
- Atp synthase epsilon chain (11 kDa, Protein from Escherichia coli)
E. coli ATP synthase imaged in 10mM MgATP State 1 "half-up" Fo refine
Sobti M, Zeng YC, Walshe JL, Brown SHJ, Ishmukhametov R, Stewart AG
Commun Biol (2023) 6 pp. 26-26 [ Pubmed: 36631659 DOI: doi:10.1038/s42003-023-04414-z ]
- Atp synthase (Complex from Escherichia coli)
E. coli ATP synthase imaged in 10mM MgATP State1 "down"
Sobti M, Zeng YC, Walshe JL, Brown SHJ, Ishmukhametov R, Stewart AG
Commun Biol (2023) 6 pp. 26-26 [ Pubmed: 36631659 DOI: doi:10.1038/s42003-023-04414-z ]
- Atp synthase (Complex from Escherichia coli)
E. coli ATP synthase imaged in 10mM MgATP State 1 "down" Fo classified
Sobti M, Zeng YC, Walshe JL, Brown SHJ, Ishmukhametov R, Stewart AG
Commun Biol (2023) 6 pp. 26-26 [ Pubmed: 36631659 DOI: doi:10.1038/s42003-023-04414-z ]
- Atp synthase (Complex from Escherichia coli)
E. coli ATP synthase imaged in 10mM MgATP State2
Sobti M, Zeng YC, Walshe JL, Brown SHJ, Ishmukhametov R, Stewart AG
Commun Biol (2023) 6 pp. 26-26 [ Pubmed: 36631659 DOI: doi:10.1038/s42003-023-04414-z ]
- Atp synthase (Complex from Escherichia coli)