Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 9gcs
Q-score: 0.276
Gjorgjevikj D, Kumar N, Wang B, Hilal T, Said N, Loll B, Artsimovitch I, Sen R, Wahl MC
Nat Commun (2025) 16 pp. 550-550 [ DOI: doi:10.1038/s41467-025-55897-9 Pubmed: 39788982 ]
- Polarity suppression protein (Complex from Enterobacteria phage P4)
- Polarity suppression protein (21 kDa, Protein from Enterobacteria phage P4)
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Transcription termination factor rho (Complex from Escherichia coli)
- Complex of e. coli transcription termination factor rho with atp and p4 polarity suppression protein psu (Complex)
Rho-ATP-Psu complex II expanded
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 9gct
Q-score: 0.274
Gjorgjevikj D, Kumar N, Wang B, Hilal T, Said N, Loll B, Artsimovitch I, Sen R, Wahl MC
Nat Commun (2025) 16 pp. 550-550 [ DOI: doi:10.1038/s41467-025-55897-9 Pubmed: 39788982 ]
- Polarity suppression protein (Complex from Enterobacteria phage P4)
- Polarity suppression protein (21 kDa, Protein from Enterobacteria phage P4)
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Transcription termination factor rho (Complex from Escherichia coli)
- Complex of e. coli transcription termination factor rho with atp and p4 polarity suppression protein psu (Complex)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 9gcu
Q-score: 0.475
Gjorgjevikj D, Kumar N, Wang B, Hilal T, Said N, Loll B, Artsimovitch I, Sen R, Wahl MC
Nat Commun (2025) 16 pp. 550-550 [ DOI: doi:10.1038/s41467-025-55897-9 Pubmed: 39788982 ]
- Water (18 Da, Ligand)
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli)
- Transcription termination factor rho p167l variant (Complex from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Complex of e. coli transcription termination factor rho p167l variant with atp gamma s (Complex)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8peu
Q-score: 0.294
Gjorgjevikj D, Kumar N, Wang B, Hilal T, Said N, Loll B, Artsimovitch I, Sen R, Wahl MC
Nat Commun (2025) 16 pp. 550-550 [ DOI: doi:10.1038/s41467-025-55897-9 Pubmed: 39788982 ]
- Polarity suppression protein (Complex from Enterobacteria phage P4)
- Polarity suppression protein (21 kDa, Protein from Enterobacteria phage P4)
- Complex of e. coli transcription termination factor rho with the atp analog, atpgammas and p4 polarity suppression protein psu (Complex)
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Transcription termination factor rho (Complex from Escherichia coli)
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 8pew
Q-score: 0.216
Gjorgjevikj D, Kumar N, Wang B, Hilal T, Said N, Loll B, Artsimovitch I, Sen R, Wahl MC
Nat Commun (2025) 16 pp. 550-550 [ DOI: doi:10.1038/s41467-025-55897-9 Pubmed: 39788982 ]
- Polarity suppression protein (Complex from Enterobacteria phage P4)
- Polarity suppression protein (21 kDa, Protein from Enterobacteria phage P4)
- Complex of e. coli transcription termination factor rho with the atp analog, atpgammas and p4 polarity suppression protein psu (Complex)
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Transcription termination factor rho (Complex from Escherichia coli)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8pex
Q-score: 0.373
Gjorgjevikj D, Kumar N, Wang B, Hilal T, Said N, Loll B, Artsimovitch I, Sen R, Wahl MC
Nat Commun (2025) 16 pp. 550-550 [ DOI: doi:10.1038/s41467-025-55897-9 Pubmed: 39788982 ]
- Polarity suppression protein (Complex from Enterobacteria phage P4)
- Polarity suppression protein (21 kDa, Protein from Enterobacteria phage P4)
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Transcription termination factor rho (Complex from Escherichia coli)
- Complex of e. coli transcription termination factor rho p167l variant with the atp analog, atpgammas and p4 polarity suppression protein psu (Complex)
Rho P167L-ATPgS-Psu complex II
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8pey
Q-score: 0.407
Gjorgjevikj D, Kumar N, Wang B, Hilal T, Said N, Loll B, Artsimovitch I, Sen R, Wahl MC
Nat Commun (2025) 16 pp. 550-550 [ DOI: doi:10.1038/s41467-025-55897-9 Pubmed: 39788982 ]
- Polarity suppression protein (Complex from Enterobacteria phage P4)
- Polarity suppression protein (21 kDa, Protein from Enterobacteria phage P4)
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Transcription termination factor rho (Complex from Escherichia coli)
- Complex of e. coli transcription termination factor rho p167l variant with the atp analog, atpgammas and p4 polarity suppression protein psu (Complex)
E. coli RNA polymerase paused at ops site
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8pdy
Q-score: 0.409
Zuber PK, Said N, Hilal T, Wang B, Loll B, Gonzalez-Higueras J, Ramirez-Sarmiento CA, Belogurov GA, Artsimovitch I, Wahl MC, Knauer SH
Nat Commun (2024) 15 pp. 3040-3040 [ DOI: doi:10.1038/s41467-024-47368-4 Pubmed: 38589445 ]
- Rna (5'-r(p*up*gp*gp*cp*gp*gp*up*ap*gp*cp*gp*u)-3') (5 kDa, RNA from Escherichia coli)
- Transcription complex (Complex from Escherichia coli)
- Template dna (35-mer) (12 kDa, DNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna and rna (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Non-template dna (35-mer) (12 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli)
- Transcription elongation complex paused at ops site, assembled on fully complementary nucleic acid scaffold (399 kDa, Complex)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8pen
Q-score: 0.451
Zuber PK, Said N, Hilal T, Wang B, Loll B, Gonzalez-Higueras J, Ramirez-Sarmiento CA, Belogurov GA, Artsimovitch I, Wahl MC, Knauer SH
Nat Commun (2024) 15 pp. 3040-3040 [ DOI: doi:10.1038/s41467-024-47368-4 Pubmed: 38589445 ]
- Transcription complex (Complex from Escherichia coli)
- Rna (5 kDa, RNA from Escherichia coli)
- Transcription antitermination protein rfah (18 kDa, Protein from Escherichia coli)
- Transcription complex paused at ops site with fully recruited rfah (417 kDa, Complex)
- Template dna (12 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Non-template dna (12 kDa, DNA from Escherichia coli)
- Dna and rna (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8pfg
Q-score: 0.471
Zuber PK, Said N, Hilal T, Wang B, Loll B, Gonzalez-Higueras J, Ramirez-Sarmiento CA, Belogurov GA, Artsimovitch I, Wahl MC, Knauer SH
Nat Commun (2024) 15 pp. 3040-3040 [ DOI: doi:10.1038/s41467-024-47368-4 Pubmed: 38589445 ]
- Transcription complex paused at ops site, assembled on not fully complementary nucleic acid scaffold, bound to disulfide-bridged (autoinhibited) rfah (417 kDa, Complex)
- Transcription complex (Complex from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Non-template dna (12 kDa, DNA from Escherichia coli)
- Dna and rna (Complex from Escherichia coli)
- Template dna (12 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Rna (5 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Transcription antitermination protein rfah (18 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)