Structure of a class A GPCR/Fab complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8tlm
Q-score: 0.527
Sun D, Sun Y, Janezic E, Zhou T, Johnson M, Azumaya C, Noreng S, Chiu C, Seki A, Arenzana TL, Nicoludis JM, Shi Y, Wang B, Ho H, Joshi P, Tam C, Payandeh J, Comps-Agrar L, Wang J, Rutz S, Koerber JT, Masureel M
Nat Commun (2023) 14 pp. 7940-7940 [ DOI: doi:10.1038/s41467-023-43601-8 Pubmed: 38040762 ]
- Fab heavy chain (23 kDa, Protein from Oryctolagus cuniculus)
- Ccr8_fab complex (Complex from Homo sapiens)
- Fab light chain (23 kDa, Protein from Oryctolagus cuniculus)
- C-c chemokine receptor type 8, green fluorescent protein fusion (76 kDa, Protein from Aequorea victoria)
Structure of a class A GPCR/agonist complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8u1u
Q-score: 0.515
Sun D, Sun Y, Janezic E, Zhou T, Johnson M, Azumaya C, Noreng S, Chiu C, Seki A, Arenzana TL, Nicoludis JM, Shi Y, Wang B, Ho H, Joshi P, Tam C, Payandeh J, Comps-Agrar L, Wang J, Rutz S, Koerber JT, Masureel M
Nat Commun (2023) 14 pp. 7940-7940 [ Pubmed: 38040762 DOI: doi:10.1038/s41467-023-43601-8 ]
- Ccr8 in complex with ccl1 and gi (Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv fragment (28 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- C-c motif chemokine 1,c-c chemokine receptor type 8,egfp fusion protein (83 kDa, Protein from Human adenovirus C serotype 2)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (43 kDa, Protein from Homo sapiens)
Human PORCN in complex with Palmitoleoyl-CoA
Resolution: 3.11 Å
EM Method: Single-particle
Fitted PDBs: 7ura
Q-score: 0.557
Liu Y, Qi X, Donnelly L, Elghobashi-Meinhardt N, Long T, Zhou RW, Sun Y, Wang B, Li X
Nature (2022) 607 pp. 816-822 [ Pubmed: 35831507 DOI: doi:10.1038/s41586-022-04952-2 ]
- Cholesterol (386 Da, Ligand)
- 2c11 light chain, 2c11 heavy chain (Complex from Mus musculus)
- Digitonin (1 kDa, Ligand)
- Zinc ion (65 Da, Ligand)
- Isoform 2 of protein-serine o-palmitoleoyltransferase porcupine (52 kDa, Protein from Homo sapiens)
- 2c11 heavy chain (26 kDa, Protein from Mus musculus)
- 2c11 light chain (25 kDa, Protein from Mus musculus)
- ~{s}-[2-[3-[[(2~{r})-4-[[[(2~{r},3~{r},4~{r},5~{r})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] (~{z})-hexadec-9-enethioate (1 kDa, Ligand)
- 16:1 coa-bound human porcn (Complex)
- Oleic acid (282 Da, Ligand)
- Isoform 2 of protein-serine o-palmitoleoyltransferase porcupine (e.c.2.3.1.250) (Complex from Homo sapiens)
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 7urc
Q-score: 0.532
Liu Y, Qi X, Donnelly L, Elghobashi-Meinhardt N, Long T, Zhou RW, Sun Y, Wang B, Li X
Nature (2022) 607 pp. 816-822 [ Pubmed: 35831507 DOI: doi:10.1038/s41586-022-04952-2 ]
- 2c11 heavy chain (26 kDa, Protein from Mus musculus)
- Isoform 2 of protein-serine o-palmitoleoyltransferase porcupine (52 kDa, Protein from Homo sapiens)
- Isoform 2 of protein-serine o-palmitoleoyltransferase porcupine (e.c.2.3.1.250) (Complex from Homo sapiens)
- 2c11 light chain (25 kDa, Protein from Mus musculus)
- Cholesterol (386 Da, Ligand)
- 2-[(2p)-2',3-dimethyl[2,4'-bipyridin]-5-yl]-n-[(5p)-5-(pyrazin-2-yl)pyridin-2-yl]acetamide (396 Da, Ligand)
- Digitonin (1 kDa, Ligand)
- 2c11 light chain, 2c11 heavy chain (Complex from Mus musculus)
- Zinc ion (65 Da, Ligand)
- Lgk974-bound human porcn (Complex)
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 7urd
Q-score: 0.569
Liu Y, Qi X, Donnelly L, Elghobashi-Meinhardt N, Long T, Zhou RW, Sun Y, Wang B, Li X
Nature (2022) 607 pp. 816-822 [ Pubmed: 35831507 DOI: doi:10.1038/s41586-022-04952-2 ]
- Isoform 2 of protein wnt-3a peptide (2 kDa, Protein from Homo sapiens)
- 2c11 heavy chain (26 kDa, Protein from Mus musculus)
- Isoform 2 of protein-serine o-palmitoleoyltransferase porcupine (52 kDa, Protein from Homo sapiens)
- Isoform 2 of protein-serine o-palmitoleoyltransferase porcupine (e.c.2.3.1.250) (Complex from Homo sapiens)
- Lgk974 and wnt3a peptide-bound human porcn (Complex)
- 2c11 light chain (25 kDa, Protein from Mus musculus)
- Cholesterol (386 Da, Ligand)
- Oleic acid (282 Da, Ligand)
- Isoform 2 of protein wnt-3a peptide (Complex)
- 2-[(2p)-2',3-dimethyl[2,4'-bipyridin]-5-yl]-n-[(5p)-5-(pyrazin-2-yl)pyridin-2-yl]acetamide (396 Da, Ligand)
- 2c11 light chain, 2c11 heavy chain (Complex from Mus musculus)
- Zinc ion (65 Da, Ligand)
- Digitonin (1 kDa, Ligand)
Human PORCN in complex with palmitoleoylated WNT3A peptide
Resolution: 3.19 Å
EM Method: Single-particle
Fitted PDBs: 7ure
Q-score: 0.539
Liu Y, Qi X, Donnelly L, Elghobashi-Meinhardt N, Long T, Zhou RW, Sun Y, Wang B, Li X
Nature (2022) 607 pp. 816-822 [ Pubmed: 35831507 DOI: doi:10.1038/s41586-022-04952-2 ]
- Protein wnt-3a (2 kDa, Protein from Homo sapiens)
- Palmitoleic acid (254 Da, Ligand)
- 2c11 heavy chain (26 kDa, Protein from Mus musculus)
- Isoform 2 of protein-serine o-palmitoleoyltransferase porcupine (52 kDa, Protein from Homo sapiens)
- Isoform 2 of protein-serine o-palmitoleoyltransferase porcupine (e.c.2.3.1.250) (Complex from Homo sapiens)
- 2c11 light chain (25 kDa, Protein from Mus musculus)
- Isoform 2 of protein wnt-3a (Complex)
- Product-bound human porcn (Complex)
- 2c11 light chain, 2c11 heavy chain (Complex from Mus musculus)
- Zinc ion (65 Da, Ligand)
- Digitonin (1 kDa, Ligand)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7urf
Q-score: 0.597
Liu Y, Qi X, Donnelly L, Elghobashi-Meinhardt N, Long T, Zhou RW, Sun Y, Wang B, Li X
Nature (2022) 607 pp. 816-822 [ Pubmed: 35831507 DOI: doi:10.1038/s41586-022-04952-2 ]
- 3h02 heavy chain, 3h02 light chain (Complex from Mus musculus)
- 3h02 heavy chain (27 kDa, Protein from Mus musculus)
- 3h02 light chain (26 kDa, Protein from Mus musculus)
- 16:0 coa and shh-n-bound human hhat (Complex)
- Protein-cysteine n-palmitoyltransferase hhat (e.c.2.3.1.-), shh-n peptide (Complex from Homo sapiens)
- Protein-cysteine n-palmitoyltransferase hhat (58 kDa, Protein from Homo sapiens)
- Shh-n peptide (693 Da, Protein from Homo sapiens)
- Digitonin (1 kDa, Ligand)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Palmitoyl-coa (1 kDa, Ligand)