dmDicer2-LoqsPD-dsRNA Dimer status
Resolution: 3.12 Å
EM Method: Single-particle
Fitted PDBs: 7w0a
Q-score: 0.51
Su S, Wang J, Deng T, Yuan X, He J, Liu N, Li X, Huang Y, Wang HW, Ma J
Nature (2022) 607 pp. 399-406 [ Pubmed: 35768513 DOI: doi:10.1038/s41586-022-04911-x ]
- Rna (32-mer) (10 kDa, RNA from Spodoptera frugiperda)
- Rna (30-mer) (10 kDa, RNA from Drosophila melanogaster)
- Dicer2-loqspd-dsrna complex at its initial binding state (Complex from Drosophila melanogaster)
- Loquacious, isoform d (38 kDa, Protein from Drosophila melanogaster)
- Dicer-2, isoform a (198 kDa, Protein from Drosophila melanogaster)
Dicer2-LoqsPD complex at apo status
Resolution: 3.33 Å
EM Method: Single-particle
Fitted PDBs: 7w0b
Q-score: 0.339
Su S, Wang J, Deng T, Yuan X, He J, Liu N, Li X, Huang Y, Wang HW, Ma J
Nature (2022) 607 pp. 399-406 [ Pubmed: 35768513 DOI: doi:10.1038/s41586-022-04911-x ]
- Loquacious, isoform d (38 kDa, Protein from Drosophila melanogaster)
- Dicer-2, isoform a (198 kDa, Protein from Drosophila melanogaster)
- Dicer2-loqspd complex at its apo status (Complex from Drosophila melanogaster)
Dicer2-Loqs-PD-dsRNA complex at early-translocation state
Resolution: 3.93 Å
EM Method: Single-particle
Fitted PDBs: 7w0c
Q-score: 0.339
Su S, Wang J, Deng T, Yuan X, He J, Liu N, Li X, Huang Y, Wang HW, Ma J
Nature (2022) 607 pp. 399-406 [ DOI: doi:10.1038/s41586-022-04911-x Pubmed: 35768513 ]
- Loquacious, isoform d (38 kDa, Protein from Drosophila melanogaster)
- Rna (35-mer) (16 kDa, RNA from Drosophila melanogaster)
- Dicer2-loqspd-dsrna complex at its early-translocation state (Complex from Drosophila melanogaster)
- Dicer-2, isoform a (198 kDa, Protein from Drosophila melanogaster)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Dicer2-LoqsPD-dsRNA complex at mid-translocation state
Resolution: 4.18 Å
EM Method: Single-particle
Fitted PDBs: 7w0d
Q-score: 0.318
Su S, Wang J, Deng T, Yuan X, He J, Liu N, Li X, Huang Y, Wang HW, Ma J
Nature (2022) 607 pp. 399-406 [ DOI: doi:10.1038/s41586-022-04911-x Pubmed: 35768513 ]
- Dicer2-loqspd-dsrna complex at its mid-translocation state (Complex from Drosophila melanogaster)
- Loquacious, isoform d (38 kDa, Protein from Drosophila melanogaster)
- Dsrna (16 kDa, RNA from Drosophila melanogaster)
- Dicer-2, isoform a (198 kDa, Protein from Drosophila melanogaster)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
dmDicer2-LoqsPD-dsRNA Active-dicing status
Resolution: 4.03 Å
EM Method: Single-particle
Fitted PDBs: 7w0e
Q-score: 0.366
Su S, Wang J, Deng T, Yuan X, He J, Liu N, Li X, Huang Y, Wang HW, Ma J
Nature (2022) 607 pp. 399-406 [ DOI: doi:10.1038/s41586-022-04911-x Pubmed: 35768513 ]
- Dicer2-loqspd-dsrna complex at its active-dicing state (Complex from Drosophila melanogaster)
- Loquacious, isoform d (38 kDa, Protein from Drosophila melanogaster)
- Dsrna (16 kDa, RNA from Drosophila melanogaster)
- Dicer-2, isoform a (198 kDa, Protein from Drosophila melanogaster)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
dmDicer2-LoqsPD-dsRNA Post-dicing status
Resolution: 4.55 Å
EM Method: Single-particle
Fitted PDBs: 7w0f
Q-score: 0.113
Su S, Wang J, Deng T, Yuan X, He J, Liu N, Li X, Huang Y, Wang HW, Ma J
Nature (2022) 607 pp. 399-406 [ Pubmed: 35768513 DOI: doi:10.1038/s41586-022-04911-x ]
- Dicer2-loqspd-dsrna complex at its post-dicing state (Complex from Drosophila melanogaster)
- Sirna (16 kDa, RNA from Drosophila melanogaster)
- Loquacious, isoform d (38 kDa, Protein from Drosophila melanogaster)
- Dicer-2, isoform a (198 kDa, Protein from Drosophila melanogaster)