Cryo-EM structure of CCR6 bound by SQA1 and OXM2
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 9d3e
Q-score: 0.499
Wasilko DJ, Gerstenberger BS, Farley KA, Li W, Alley J, Schnute ME, Unwalla RJ, Victorino J, Crouse KK, Ding R, Sahasrabudhe PV, Vincent F, Frisbie RK, Dermenci A, Flick A, Choi C, Chinigo G, Mousseau JJ, Trujillo JI, Nuhant P, Mondal P, Lombardo V, Lamb D, Hogan BJ, Minhas GS, Segala E, Oswald C, Windsor IW, Han S, Rappas M, Cooke RM, Calabrese MF, Berstein G, Thorarensen A, Wu H
Nat Commun (2024) 15 pp. 7574-7574 [ Pubmed: 39217154 DOI: doi:10.1038/s41467-024-52045-7 ]
- Anti-bril fab nanobody (15 kDa, Protein from synthetic construct)
- Anti-bril fab light chain (25 kDa, Protein from Homo sapiens)
- 4-[[3,4-bis(oxidanylidene)-2-[[(1~{r})-1-(4-propan-2-ylfuran-2-yl)propyl]amino]cyclobuten-1-yl]amino]-~{n},~{n}-dimethyl-3-oxidanyl-pyridine-2-carboxamide (426 Da, Ligand)
- Human ccr6 (54 kDa, Protein from Homo sapiens)
- N-{[(2r)-4-(bicyclo[1.1.1]pentan-1-yl)-5-oxomorpholin-2-yl]methyl}-1-[4-(trifluoromethyl)phenyl]cyclopropane-1-carboxamide (408 Da, Ligand)
- Ccr6-bril/fab/nb in complex with sqa1 and oxm2 (120 MDa, Complex from Homo sapiens)
- Anti-bril fab heavy chain (28 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Cryo-EM structure of CCR6 bound by SQA1 and OXM1
Wasilko DJ, Gerstenberger BS, Farley KA, Li W, Alley J, Schnute ME, Unwalla RJ, Victorino J, Crouse KK, Ding R, Sahasrabudhe PV, Vincent F, Frisbie RK, Dermenci A, Flick A, Choi C, Chinigo G, Mousseau JJ, Trujillo JI, Nuhant P, Mondal P, Lombardo V, Lamb D, Hogan BJ, Minhas GS, Segala E, Oswald C, Windsor IW, Han S, Rappas M, Cooke RM, Calabrese MF, Berstein G, Thorarensen A, Wu H
Nat Commun (2024) 15 pp. 7574-7574 [ Pubmed: 39217154 DOI: doi:10.1038/s41467-024-52045-7 ]
- Anti-bril fab nanobody (15 kDa, Protein from synthetic construct)
- Anti-bril fab light chain (25 kDa, Protein from Homo sapiens)
- 1-(4-chlorophenyl)-n-{[(2r)-4-(2,3-dihydro-1h-inden-2-yl)-5-oxomorpholin-2-yl]methyl}cyclopropane-1-carboxamide (424 Da, Ligand)
- Ccr6-bril/fab/nb in complex with sqa1 and oxm1 (120 MDa, Complex from Homo sapiens)
- Ccr6, soluble cytochrome b562 (54 kDa, Protein from Homo sapiens)
- 4-[[3,4-bis(oxidanylidene)-2-[[(1~{r})-1-(4-propan-2-ylfuran-2-yl)propyl]amino]cyclobuten-1-yl]amino]-~{n},~{n}-dimethyl-3-oxidanyl-pyridine-2-carboxamide (426 Da, Ligand)
- Anti-bril fab heavy chain (28 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8e4y
Q-score: 0.502
Johnson ZL, Ammirati M, Wasilko DJ, Chang JS, Noell S, Foley TL, Yoon H, Smith K, Asano S, Hales K, Wan M, Yang Q, Piotrowski MA, Farley KA, Gilbert T, Aschenbrenner LM, Fennell KF, Dutra JK, Xu M, Guo C, Varghese AE, Bellenger J, Quinn A, Am Ende CW, West GM, Griffor MC, Bennett D, Calabrese M, Steppan CM, Han S, Wu H
Nat Struct Mol Biol (2023) 30 pp. 22-30 [ DOI: doi:10.1038/s41594-022-00884-7 Pubmed: 36522428 ]
- Glycerol-3-phosphate acyltransferase 1 (86 kDa, Complex from Homo sapiens)
- Glycerol-3-phosphate acyltransferase 1, mitochondrial (86 kDa, Protein from Homo sapiens)
- (2r)-2-hydroxy-3-(phosphonooxy)propyl hexadecanoate (410 Da, Ligand)
- [(2r,3s,4r,5r)-5-(6-amino-9h-purin-9-yl)-4-hydroxy-3-(phosphonooxy)oxolan-2-yl]methyl (3r)-3-hydroxy-2,2-dimethyl-4-oxo-4-{[3-oxo-3-({2-[(2-oxohexadecyl)sulfanyl]ethyl}amino)propyl]amino}butyl dihydrogen diphosphate (non-preferred name) (1 kDa, Ligand)
Resolution: 3.67 Å
EM Method: Single-particle
Fitted PDBs: 8e50
Q-score: 0.444
Johnson ZL, Ammirati M, Wasilko DJ, Chang JS, Noell S, Foley TL, Yoon H, Smith K, Asano S, Hales K, Wan M, Yang Q, Piotrowski MA, Farley KA, Gilbert T, Aschenbrenner LM, Fennell KF, Dutra JK, Xu M, Guo C, Varghese AE, Bellenger J, Quinn A, Am Ende CW, West GM, Griffor MC, Bennett D, Calabrese M, Steppan CM, Han S, Wu H
Nat Struct Mol Biol (2023) 30 pp. 22-30 [ DOI: doi:10.1038/s41594-022-00884-7 Pubmed: 36522428 ]
- Glycerol-3-phosphate acyltransferase 1 (86 kDa, Complex from Homo sapiens)
- Glycerol-3-phosphate acyltransferase 1, mitochondrial (86 kDa, Protein from Homo sapiens)
- (2r)-2-hydroxy-3-(phosphonooxy)propyl hexadecanoate (410 Da, Ligand)
- Coenzyme a (767 Da, Ligand)
Cryo-EM structure of the human chemokine receptor CCR6 in complex with CCL20 and a Go protein
Resolution: 3.34 Å
EM Method: Single-particle
Fitted PDBs: 6wwz
Q-score: 0.505
Wasilko DJ, Johnson ZL, Ammirati M, Che Y, Griffor MC, Han S, Wu H
Nat Commun (2020) 11 pp. 3031-3031 [ Pubmed: 32541785 DOI: doi:10.1038/s41467-020-16820-6 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Rattus norvegicus)
- C-c motif chemokine 20 (8 kDa, Protein from Homo sapiens)
- C-c motif chemokine 20, guanine nucleotide-binding protein g(o) subunit alpha,guanine nucleotide-binding protein g(o) subunit alpha, c-c chemokine receptor type 6,c-c chemokine receptor type 6 (Complex from Homo sapiens)
- Scfv16 (30 kDa, Protein from Mus musculus)
- Ccr6-ccl20-go complex (Complex)
- Guanine nucleotide-binding protein g(o) subunit alpha,guanine nucleotide-binding protein g(o) subunit alpha (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Rattus norvegicus)
- Scfv16 (Complex from Mus musculus)
- C-c chemokine receptor type 6,c-c chemokine receptor type 6 (60 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Rattus norvegicus)