Cryo-EM structure of CCR6 bound by SQA1 and OXM2
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 9d3e
Q-score: 0.499
Wasilko DJ, Gerstenberger BS, Farley KA, Li W, Alley J, Schnute ME, Unwalla RJ, Victorino J, Crouse KK, Ding R, Sahasrabudhe PV, Vincent F, Frisbie RK, Dermenci A, Flick A, Choi C, Chinigo G, Mousseau JJ, Trujillo JI, Nuhant P, Mondal P, Lombardo V, Lamb D, Hogan BJ, Minhas GS, Segala E, Oswald C, Windsor IW, Han S, Rappas M, Cooke RM, Calabrese MF, Berstein G, Thorarensen A, Wu H
Nat Commun (2024) 15 pp. 7574-7574 [ DOI: doi:10.1038/s41467-024-52045-7 Pubmed: 39217154 ]
- Ccr6-bril/fab/nb in complex with sqa1 and oxm2 (120 MDa, Complex from Homo sapiens)
- Human ccr6 (54 kDa, Protein from Homo sapiens)
- Anti-bril fab nanobody (15 kDa, Protein from synthetic construct)
- Anti-bril fab light chain (25 kDa, Protein from Homo sapiens)
- N-{[(2r)-4-(bicyclo[1.1.1]pentan-1-yl)-5-oxomorpholin-2-yl]methyl}-1-[4-(trifluoromethyl)phenyl]cyclopropane-1-carboxamide (408 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- 4-[[3,4-bis(oxidanylidene)-2-[[(1~{r})-1-(4-propan-2-ylfuran-2-yl)propyl]amino]cyclobuten-1-yl]amino]-~{n},~{n}-dimethyl-3-oxidanyl-pyridine-2-carboxamide (426 Da, Ligand)
- Anti-bril fab heavy chain (28 kDa, Protein from Homo sapiens)
Cryo-EM structure of CCR6 bound by SQA1 and OXM1
Wasilko DJ, Gerstenberger BS, Farley KA, Li W, Alley J, Schnute ME, Unwalla RJ, Victorino J, Crouse KK, Ding R, Sahasrabudhe PV, Vincent F, Frisbie RK, Dermenci A, Flick A, Choi C, Chinigo G, Mousseau JJ, Trujillo JI, Nuhant P, Mondal P, Lombardo V, Lamb D, Hogan BJ, Minhas GS, Segala E, Oswald C, Windsor IW, Han S, Rappas M, Cooke RM, Calabrese MF, Berstein G, Thorarensen A, Wu H
Nat Commun (2024) 15 pp. 7574-7574 [ DOI: doi:10.1038/s41467-024-52045-7 Pubmed: 39217154 ]
- Ccr6, soluble cytochrome b562 (54 kDa, Protein from Homo sapiens)
- 1-(4-chlorophenyl)-n-{[(2r)-4-(2,3-dihydro-1h-inden-2-yl)-5-oxomorpholin-2-yl]methyl}cyclopropane-1-carboxamide (424 Da, Ligand)
- Anti-bril fab nanobody (15 kDa, Protein from synthetic construct)
- Anti-bril fab light chain (25 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Ccr6-bril/fab/nb in complex with sqa1 and oxm1 (120 MDa, Complex from Homo sapiens)
- 4-[[3,4-bis(oxidanylidene)-2-[[(1~{r})-1-(4-propan-2-ylfuran-2-yl)propyl]amino]cyclobuten-1-yl]amino]-~{n},~{n}-dimethyl-3-oxidanyl-pyridine-2-carboxamide (426 Da, Ligand)
- Anti-bril fab heavy chain (28 kDa, Protein from Homo sapiens)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8e4y
Q-score: 0.502
Johnson ZL, Ammirati M, Wasilko DJ, Chang JS, Noell S, Foley TL, Yoon H, Smith K, Asano S, Hales K, Wan M, Yang Q, Piotrowski MA, Farley KA, Gilbert T, Aschenbrenner LM, Fennell KF, Dutra JK, Xu M, Guo C, Varghese AE, Bellenger J, Quinn A, Am Ende CW, West GM, Griffor MC, Bennett D, Calabrese M, Steppan CM, Han S, Wu H
Nat Struct Mol Biol (2023) 30 pp. 22-30 [ Pubmed: 36522428 DOI: doi:10.1038/s41594-022-00884-7 ]
- [(2r,3s,4r,5r)-5-(6-amino-9h-purin-9-yl)-4-hydroxy-3-(phosphonooxy)oxolan-2-yl]methyl (3r)-3-hydroxy-2,2-dimethyl-4-oxo-4-{[3-oxo-3-({2-[(2-oxohexadecyl)sulfanyl]ethyl}amino)propyl]amino}butyl dihydrogen diphosphate (non-preferred name) (1 kDa, Ligand)
- (2r)-2-hydroxy-3-(phosphonooxy)propyl hexadecanoate (410 Da, Ligand)
- Glycerol-3-phosphate acyltransferase 1, mitochondrial (86 kDa, Protein from Homo sapiens)
- Glycerol-3-phosphate acyltransferase 1 (86 kDa, Complex from Homo sapiens)
Resolution: 3.67 Å
EM Method: Single-particle
Fitted PDBs: 8e50
Q-score: 0.444
Johnson ZL, Ammirati M, Wasilko DJ, Chang JS, Noell S, Foley TL, Yoon H, Smith K, Asano S, Hales K, Wan M, Yang Q, Piotrowski MA, Farley KA, Gilbert T, Aschenbrenner LM, Fennell KF, Dutra JK, Xu M, Guo C, Varghese AE, Bellenger J, Quinn A, Am Ende CW, West GM, Griffor MC, Bennett D, Calabrese M, Steppan CM, Han S, Wu H
Nat Struct Mol Biol (2023) 30 pp. 22-30 [ Pubmed: 36522428 DOI: doi:10.1038/s41594-022-00884-7 ]
- Coenzyme a (767 Da, Ligand)
- (2r)-2-hydroxy-3-(phosphonooxy)propyl hexadecanoate (410 Da, Ligand)
- Glycerol-3-phosphate acyltransferase 1, mitochondrial (86 kDa, Protein from Homo sapiens)
- Glycerol-3-phosphate acyltransferase 1 (86 kDa, Complex from Homo sapiens)
Cryo-EM structure of the human chemokine receptor CCR6 in complex with CCL20 and a Go protein
Resolution: 3.34 Å
EM Method: Single-particle
Fitted PDBs: 6wwz
Q-score: 0.505
Wasilko DJ, Johnson ZL, Ammirati M, Che Y, Griffor MC, Han S, Wu H
Nat Commun (2020) 11 pp. 3031-3031 [ Pubmed: 32541785 DOI: doi:10.1038/s41467-020-16820-6 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Rattus norvegicus)
- Ccr6-ccl20-go complex (Complex)
- Scfv16 (Complex from Mus musculus)
- C-c motif chemokine 20 (8 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(o) subunit alpha,guanine nucleotide-binding protein g(o) subunit alpha (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Rattus norvegicus)
- Scfv16 (30 kDa, Protein from Mus musculus)
- C-c motif chemokine 20, guanine nucleotide-binding protein g(o) subunit alpha,guanine nucleotide-binding protein g(o) subunit alpha, c-c chemokine receptor type 6,c-c chemokine receptor type 6 (Complex from Homo sapiens)
- C-c chemokine receptor type 6,c-c chemokine receptor type 6 (60 kDa, Protein from Homo sapiens)