DNA-directed RNA polymerase with transcriptional activator PafBC
Schilling CM, Zdanowicz R, Rabl J, Muller AU, Boehringer D, Glockshuber R, Weber-Ban E
Sci Adv (2025) 11 pp. eadq9054-eadq9054 [ Pubmed: 39919186 DOI: doi:10.1126/sciadv.adq9054 ]
- Dna-directed rna polymerase with transcriptional activator pafbc (Complex from Mycolicibacterium smegmatis MC2 155)
- Dna-directed rna polymerase subunit beta' (Protein from Mycolicibacterium smegmatis MC2 155)
- Pafc (Protein from Mycolicibacterium smegmatis MC2 155)
- Rna polymerase sigma factor siga (Protein from Mycolicibacterium smegmatis MC2 155)
- Rna polymerase-binding protein rbpa (Protein from Mycolicibacterium smegmatis MC2 155)
- Pafb (Protein from Mycolicibacterium smegmatis MC2 155)
- Reca-op template strand (DNA from Mycolicibacterium smegmatis MC2 155)
- Dna-directed rna polymerase subunit alpha (Protein from Mycolicibacterium smegmatis MC2 155)
- Reca-op non-template strand (DNA from Mycolicibacterium smegmatis MC2 155)
- Dna-directed rna polymerase subunit omega (Protein from Mycolicibacterium smegmatis MC2 155)
- Dna-directed rna polymerase subunit beta (Protein from Mycolicibacterium smegmatis MC2 155)
Transcriptional activator PafBC bound to mycobacterial RNA polymerase
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 9fnd
Q-score: 0.332
Schilling CM, Zdanowicz R, Rabl J, Muller AU, Boehringer D, Glockshuber R, Weber-Ban E
Sci Adv (2025) 11 pp. eadq9054-eadq9054 [ Pubmed: 39919186 DOI: doi:10.1126/sciadv.adq9054 ]
- Pafc (34 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Pafbc (Complex from Mycolicibacterium smegmatis MC2 155)
- Transcriptional activator pafbc bound to mycobacterial rna polymerase (Complex from Mycolicibacterium smegmatis MC2 155)
- Transcriptional regulator-like protein (36 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Rnap polymerase sigma factor a and rna polymerase-binding protein rbpa (Complex from Mycolicibacterium smegmatis MC2 155)
- Dna-directed rna polymerase (Complex from Mycolicibacterium smegmatis MC2 155)
Composite map of the mycobacterial PafBC-bound transcription initiation complex
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 9fne
Q-score: 0.415
Schilling CM, Zdanowicz R, Rabl J, Muller AU, Boehringer D, Glockshuber R, Weber-Ban E
Sci Adv (2025) 11 pp. eadq9054-eadq9054 [ Pubmed: 39919186 DOI: doi:10.1126/sciadv.adq9054 ]
- Dna-directed rna polymerase subunit beta' (146 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Rna polymerase-binding protein rbpa (13 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Dna-directed rna polymerase with transcriptional activator pafbc (Complex from Mycolicibacterium smegmatis MC2 155)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Dna-directed rna polymerase subunit beta (128 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Reca-op non-template strand (21 kDa, DNA from Mycolicibacterium smegmatis MC2 155)
- Pafc (34 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Zinc ion (65 Da, Ligand)
- Transcriptional regulator-like protein (36 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Rna polymerase sigma factor siga (51 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Reca-op template strand (20 kDa, DNA from Mycolicibacterium smegmatis MC2 155)
- Magnesium ion (24 Da, Ligand)
Substrate-engaged mycobacterial Proteasome-associated ATPase - focused 3D refinement (state A)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7px9
Q-score: 0.402
Kavalchuk M, Jomaa A, Muller AU, Weber-Ban E
Nat Commun (2022) 13 pp. 276-276 [ Pubmed: 35022401 DOI: doi:10.1038/s41467-021-27787-3 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Prokaryotic ubiquitin-like protein pup (7 kDa, Protein from Mycobacterium tuberculosis)
- Aaa atpase forming ring-shaped complexes (67 kDa, Protein from Mycobacterium tuberculosis)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Mycobacterial proteasome-associated atpase in complex with substrate and open-gate 20scp (1 MDa, Complex from Mycobacterium tuberculosis)
Open-gate mycobacterium 20S CP proteasome in complex MPA - global 3D refinement
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7pxa
Q-score: 0.555
Kavalchuk M, Jomaa A, Muller AU, Weber-Ban E
Nat Commun (2022) 13 pp. 276-276 [ Pubmed: 35022401 DOI: doi:10.1038/s41467-021-27787-3 ]
- Aaa atpase forming ring-shaped complexes (67 kDa, Protein from Mycobacterium tuberculosis)
- Mycobacterial proteasome-associated atpase in complex with substrate and open-gate 20scp (1 MDa, Complex from Mycobacterium tuberculosis)
- Proteasome subunit alpha (26 kDa, Protein from Mycobacterium tuberculosis)
- Proteasome subunit beta (30 kDa, Protein from Mycobacterium tuberculosis)
Substrate-engaged mycobacterial Proteasome-associated ATPase - focused 3D refinement (state B)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7pxb
Q-score: 0.35
Kavalchuk M, Jomaa A, Muller AU, Weber-Ban E
Nat Commun (2022) 13 pp. 276-276 [ Pubmed: 35022401 DOI: doi:10.1038/s41467-021-27787-3 ]
- Prokaryotic ubiquitin-like protein pup (7 kDa, Protein from Mycobacterium tuberculosis)
- Aaa atpase forming ring-shaped complexes (67 kDa, Protein from Mycobacterium tuberculosis)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Mycobacterial proteasome-associated atpase in complex with substrate and open-gate 20scp (1 MDa, Complex from Mycobacterium tuberculosis)
Resolution: 3.84 Å
EM Method: Single-particle
Fitted PDBs: 7pxc
Q-score: 0.474
Kavalchuk M, Jomaa A, Muller AU, Weber-Ban E
Nat Commun (2022) 13 pp. 276-276 [ Pubmed: 35022401 DOI: doi:10.1038/s41467-021-27787-3 ]
- Proteasome subunit alpha (26 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Proteasome subunit beta (30 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Prokaryotic ubiquitin-like protein pup (7 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Proteasome-associated atpase (67 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Mycobacterial proteasome-associated atpase in complex with substrate and open-gate 20scp (1 MDa, Complex from Mycobacterium tuberculosis)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7pxd
Q-score: 0.453
Kavalchuk M, Jomaa A, Muller AU, Weber-Ban E
Nat Commun (2022) 13 pp. 276-276 [ Pubmed: 35022401 DOI: doi:10.1038/s41467-021-27787-3 ]
- Prokaryotic ubiquitin-like protein pup (7 kDa, Protein from Mycobacterium tuberculosis)
- Aaa atpase forming ring-shaped complexes (67 kDa, Protein from Mycobacterium tuberculosis)
- Proteasome subunit beta (30 kDa, Protein from Mycobacterium tuberculosis)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Proteasome subunit alpha (26 kDa, Protein from Mycobacterium tuberculosis)
- Magnesium ion (24 Da, Ligand)
- Mycobacterial proteasome-associated atpase in complex with substrate and open-gate 20scp (1 MDa, Complex from Mycobacterium tuberculosis)
transcriptional activator PafBC bound to mycobacterial RNA polymerase
Muller AU, Kummer E, Schilling CM, Ban N, Weber-Ban E
Sci Adv (2021) 7 pp. eabl4064-eabl4064 [ Pubmed: 34851662 DOI: doi:10.1126/sciadv.abl4064 ]
- Rna polymerase sigma factor siga, rna polymerase-binding protein rbpa and rna polymerase-binding transcription factor card (Complex from Mycobacterium smegmatis)
- Dna-directed rna polymerase with transcriptional activator pafbc (Complex)
- Pafb and pafc (Complex from Mycobacterium smegmatis)
- Dna-dependent rna polymerase (Complex from Mycobacterium smegmatis)
Mycobacterial RNAP with transcriptional activator PafBC
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7p5x
Q-score: 0.532
Muller AU, Kummer E, Schilling CM, Ban N, Weber-Ban E
Sci Adv (2021) 7 pp. eabl4064-eabl4064 [ Pubmed: 34851662 DOI: doi:10.1126/sciadv.abl4064 ]
- Dna-directed rna polymerase subunit beta' (146 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Rna polymerase-binding protein rbpa (13 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Pafc (34 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Dna-directed rna polymerase subunit beta (128 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Reca-op template strand (23 kDa, DNA from Mycolicibacterium smegmatis MC2 155)
- Zinc ion (65 Da, Ligand)
- Rna polymerase sigma factor siga (51 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Dna-directed rna polymerase with transcriptional activator pafbc (Complex)
- Reca-op non-template strand (23 kDa, DNA from Mycolicibacterium smegmatis MC2 155)
- Transcriptional regulator-like protein (36 kDa, Protein from Mycolicibacterium smegmatis MC2 155)