SARS-CoV-2 endoribonuclease Nsp15 bound to dsRNA with 1 nucleotide bulge
Resolution: 3.24 Å
EM Method: Single-particle
Fitted PDBs: 9bih
Q-score: 0.266
Wright ZM, Butay KJ, Krahn JM, Wilson IM, Gabel SA, DeRose EF, Hissein IS, Williams JG, Borgnia MJ, Frazier MN, Mueller GA, Stanley RE
Nat Commun (2025) 16 pp. 391-391 [ Pubmed: 39755678 DOI: doi:10.1038/s41467-024-55682-0 ]
- Rna (35-mer) (11 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Uridylate-specific endoribonuclease nsp15 (39 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 endoribonuclease nsp15 bound to dsrna with 1 nucleotide bulge (Complex from Severe acute respiratory syndrome coronavirus 2)
- Rna (34-mer) (10 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
SARS-CoV-2 endoribonuclease Nsp15 bound to dsRNA
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7tj2
Q-score: 0.459
Frazier MN, Wilson IM, Krahn JM, Butay KJ, Dillard LB, Borgnia MJ, Stanley RE
Nucleic Acids Res (2022) 50 pp. 8290-8301 [ Pubmed: 35801916 DOI: doi:10.1093/nar/gkac589 ]
- Rna (31-mer) (16 kDa, RNA from synthetic construct)
- Uridylate-specific endoribonuclease nsp15 (39 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Structural basis for dsrna cleavage by the sars-cov-2 endoribonuclease (250 kDa, Complex from Severe acute respiratory syndrome coronavirus 2)
- Rna (31-mer) (16 kDa, RNA from synthetic construct)
SARS-CoV-2 endoribonuclease Nsp15 bound to dsRNA
Resolution: 3.43 Å
EM Method: Single-particle
Fitted PDBs: 7tqv
Q-score: 0.387
Frazier MN, Wilson IM, Krahn JM, Butay KJ, Dillard LB, Borgnia MJ, Stanley RE
Nucleic Acids Res (2022) 50 pp. 8290-8301 [ Pubmed: 35801916 DOI: doi:10.1093/nar/gkac589 ]
- Rna (33-mer) (16 kDa, RNA from unidentified)
- Rna (33-mer) (16 kDa, RNA from unidentified)
- Nsp15/dsrna (250 kDa, Complex from Severe acute respiratory syndrome coronavirus 2)
- Uridylate-specific endoribonuclease (40 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
SARS-CoV-2 Nsp15 endoribonuclease pre-cleavage state
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7n33
Q-score: 0.606
Frazier MN, Dillard LB, Krahn JM, Perera L, Williams JG, Wilson IM, Stewart ZD, Pillon MC, Deterding LJ, Borgnia MJ, Stanley RE
Nucleic Acids Res (2021) 49 pp. 10136-10149 [ DOI: doi:10.1093/nar/gkab719 Pubmed: 34403466 ]
SARS-CoV-2 Nsp15 endoribonuclease post-cleavage state
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 7n06
Q-score: 0.703
Frazier MN, Dillard LB, Krahn JM, Perera L, Williams JG, Wilson IM, Stewart ZD, Pillon MC, Deterding LJ, Borgnia MJ, Stanley RE
Nucleic Acids Res (2021) 49 pp. 10136-10149 [ DOI: doi:10.1093/nar/gkab719 Pubmed: 34403466 ]
- Rna (5'-r(*ap*up*a)-3') (919 Da, RNA from Homo sapiens)
- Water (18 Da, Ligand)
- Nsp15 viral endoribonuclease in a post-cleavage state (250 kDa, Complex from Severe acute respiratory syndrome coronavirus 2)
- Uridylate-specific endoribonuclease (42 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)