Resolution: 3.05 Å
EM Method: Single-particle
Fitted PDBs: 7nf6
Q-score: 0.558
Lee Y, Wiriyasermkul P, Kongpracha P, Moriyama S, Mills DJ, Kuhlbrandt W, Nagamori S
Nat Commun (2022) 13 pp. 2708-2708 [ Pubmed: 35577790 DOI: doi:10.1038/s41467-022-30293-9 ]
- B0,+at-rbat heterodimer (135 MDa, Complex from Ovis aries)
- B(0,+)-type amino acid transporter 1 (56 kDa, Protein from Ovis aries)
- Neutral and basic amino acid transport protein rbat (78 kDa, Protein from Ovis aries)
- Cholesterol (386 Da, Ligand)
- 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (760 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Ovine rBAT ectodomain homodimer, C2-symmetry
Resolution: 2.68 Å
EM Method: Single-particle
Fitted PDBs: 7nf7
Q-score: 0.642
Lee Y, Wiriyasermkul P, Kongpracha P, Moriyama S, Mills DJ, Kuhlbrandt W, Nagamori S
Nat Commun (2022) 13 pp. 2708-2708 [ Pubmed: 35577790 DOI: doi:10.1038/s41467-022-30293-9 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Neutral and basic amino acid transport protein rbat (78 kDa, Protein from Ovis aries)
- Ovine rbat ectodomain homodimer (79 MDa, Complex from Ovis aries)
- Calcium ion (40 Da, Ligand)
Ovine (b0,+AT-rBAT)2 hetero-tetramer, consensus refinement, C2-symmetry
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 7nf8
Q-score: 0.496
Lee Y, Wiriyasermkul P, Kongpracha P, Moriyama S, Mills DJ, Kuhlbrandt W, Nagamori S
Nat Commun (2022) 13 pp. 2708-2708 [ Pubmed: 35577790 DOI: doi:10.1038/s41467-022-30293-9 ]
- B(0,+)-type amino acid transporter 1 (56 kDa, Protein from Ovis aries)
- Neutral and basic amino acid transport protein rbat (78 kDa, Protein from Ovis aries)
- Cholesterol (386 Da, Ligand)
- 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (760 Da, Ligand)
- (b0,+at-rbat) hetero-tetramer (270 MDa, Complex from Ovis aries)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Consensus mutated xCT-CD98hc complex
Resolution: 6.2 Å
EM Method: Single-particle
Fitted PDBs: 7ccs
Q-score: 0.162
Oda K, Lee Y, Wiriyasermkul P, Tanaka Y, Takemoto M, Yamashita K, Nagamori S, Nishizawa T, Nureki O
Protein Sci (2020) 29 pp. 2398-2407 [ DOI: doi:10.1002/pro.3966 Pubmed: 33016372 ]
- Consensus mutated xct-cd98hc complex (120 kDa, Complex from Homo sapiens)
- 4f2 cell-surface antigen heavy chain (68 kDa, Protein from Homo sapiens)
- Consensus mutated anionic amino acid transporter light chain, xc- system (55 kDa, Protein from Homo sapiens)
LAT1-CD98hc bound to MEM-108 Fab
Lee Y, Wiriyasermkul P, Jin C, Quan L, Ohgaki R, Okuda S, Kusakizako T, Nishizawa T, Oda K, Ishitani R, Yokoyama T, Nakane T, Shirouzu M, Endou H, Nagamori S, Kanai Y, Nureki O
Nat. Struct. Mol. Biol. (2019) [ DOI: 10.1038/s41594-019-0237-7 Pubmed: 31160781 ]
Image sets:
LAT1-CD98hc complex bound to MEM-108 Fab
Resolution: 3.31 Å
EM Method: Single-particle
Fitted PDBs: 6jmq
Q-score: 0.487
Lee Y, Wiriyasermkul P, Jin C, Quan L, Ohgaki R, Okuda S, Kusakizako T, Nishizawa T, Oda K, Ishitani R, Yokoyama T, Nakane T, Shirouzu M, Endou H, Nagamori S, Kanai Y, Nureki O
Nat Struct Mol Biol (2019) 26 pp. 510-517 [ Pubmed: 31160781 DOI: doi:10.1038/s41594-019-0237-7 ]
- 4f2 cell-surface antigen heavy chain (68 kDa, Protein from Homo sapiens)
- Antibody (22 kDa, Protein from Mus musculus)
- Lat1-cd98hc complex bound to mem-108 fab (175 MDa, Complex from Homo sapiens)
- Large neutral amino acids transporter small subunit 1 (56 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Antibody (23 kDa, Protein from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
CD98hc extracellular domain bound to HBJ127 Fab and MEM-108 Fab
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6jmr
Q-score: 0.398
Lee Y, Wiriyasermkul P, Jin C, Quan L, Ohgaki R, Okuda S, Kusakizako T, Nishizawa T, Oda K, Ishitani R, Yokoyama T, Nakane T, Shirouzu M, Endou H, Nagamori S, Kanai Y, Nureki O
Nat Struct Mol Biol (2019) 26 pp. 510-517 [ Pubmed: 31160781 DOI: doi:10.1038/s41594-019-0237-7 ]
- 4f2 cell-surface antigen heavy chain (68 kDa, Protein from Homo sapiens)
- Antibody (22 kDa, Protein from Mus musculus)
- Antibody (23 kDa, Protein from Mus musculus)
- Cd98hc extracellular domain bound to mem-108 fab and hbj127 fab (225 MDa, Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Antibody (23 kDa, Protein from Mus musculus)
- Antibody (24 kDa, Protein from Mus musculus)