Complex between MLL1-WRAD and an H2B-ubiquitinated nucleosome- State 1
Rahman S, Hoffmann NA, Worden EJ, Smith ML, Namitz KEW, Knutson BA, Cosgrove MS, Wolberger C
PNAS (2022) 119 pp. e2205691119-e2205691119 [ DOI: doi:10.1073/pnas.2205691119 Pubmed: 36095189 ]
Complex between RbBP5-WDR5 and an H2B-ubiquitinated nucleosome
Resolution: 3.55 Å
EM Method: Single-particle
Fitted PDBs: 8du4
Q-score: 0.237
Rahman S, Hoffmann NA, Worden EJ, Smith ML, Namitz KEW, Knutson BA, Cosgrove MS, Wolberger C
PNAS (2022) 119 pp. e2205691119-e2205691119 [ DOI: doi:10.1073/pnas.2205691119 Pubmed: 36095189 ]
- Rbbp5-wdr5 complex (Complex from Homo sapiens)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Histone h2a (14 kDa, Protein from Xenopus laevis)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Retinoblastoma-binding protein 5 (59 kDa, Protein from Homo sapiens)
- Wd repeat-containing protein 5 (36 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- 601 dna (146-mer) (45 kDa, DNA from synthetic construct)
- Ubiquitinated nucleosome (Complex from Xenopus laevis)
- 601 dna (146-mer) (44 kDa, DNA from synthetic construct)
- Polyubiquitin-b (9 kDa, Protein from Homo sapiens)
- Rbbp5-wdr5 complex bound to the ubiquitinated nucleosome (Complex)
Poised-state Dot1L bound to the H2B-Ubiquitinated nucleosome
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6nog
Q-score: 0.356
Worden EJ, Hoffmann NA, Hicks CW, Wolberger C
Cell (2019) 176 pp. 1490-1501.e12 [ Pubmed: 30765112 DOI: doi:10.1016/j.cell.2019.02.002 ]
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (47 kDa, Protein from Homo sapiens)
- Ubiquitin (Complex from Homo sapiens)
- Poised state dot1l in complex with the h2b-ub nucleosome (Complex)
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- H2b-ub nucleosome (Complex)
- Histone h2a type 1 (13 kDa, Protein from Xenopus laevis)
- Ubiquitin (9 kDa, Protein from Homo sapiens)
- Dna (Complex from synthetic construct)
- 601 dna strand 2 (45 kDa, DNA from synthetic construct)
- Dot1l (Complex from Homo sapiens)
- 601 dna strand 1 (44 kDa, DNA from synthetic construct)
- Histone core (Complex from Xenopus laevis)
Active state Dot1L bound to the H2B-Ubiquitinated nucleosome, 1-to-1 complex
Resolution: 3.54 Å
EM Method: Single-particle
Fitted PDBs: 6nqa
Q-score: 0.436
Worden EJ, Hoffmann NA, Hicks CW, Wolberger C
Cell (2019) 176 pp. 1490-1501.e12 [ Pubmed: 30765112 DOI: doi:10.1016/j.cell.2019.02.002 ]
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Active state dot1l in complex with the h2b-ub nucleosome (Complex)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (47 kDa, Protein from Homo sapiens)
- Dot1l (Complex from Xenopus laevis)
- S-adenosylmethionine (398 Da, Ligand)
- Ubiquitin (Complex from Homo sapiens)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- H2b-ub nucleosome (Complex)
- Histone h2a type 1 (13 kDa, Protein from Xenopus laevis)
- 601 dna strand 2 (45 kDa, DNA from synthetic construct)
- Dna (Complex from synthetic construct)
- Ubiquitin (9 kDa, Protein from Homo sapiens)
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- 601 dna strand 1 (44 kDa, DNA from synthetic construct)
- Histone core (Complex from Xenopus laevis)