Qian Y, Ma Z, Xu Z, Duan Y, Xiong Y, Xia R, Zhu X, Zhang Z, Tian X, Yin H, Liu J, Song J, Lu Y, Zhang A, Guo C, Jin L, Kim WJ, Ke J, Xu F, Huang Z, He Y
Nat Commun (2024) 15 pp. 7644-7644 [ Pubmed: 39223191 DOI: doi:10.1038/s41467-024-52174-z ]
- Segment polarity protein dishevelled homolog dvl-2 (79 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Frizzled-4 (60 kDa, Protein from Homo sapiens)
- Gpcr complex (450 kDa, Complex from Homo sapiens)
Fzd4/DEP complex (local refined)
Qian Y, Ma Z, Xu Z, Duan Y, Xiong Y, Xia R, Zhu X, Zhang Z, Tian X, Yin H, Liu J, Song J, Lu Y, Zhang A, Guo C, Jin L, Kim WJ, Ke J, Xu F, Huang Z, He Y
Nat Commun (2024) 15 pp. 7644-7644 [ Pubmed: 39223191 DOI: doi:10.1038/s41467-024-52174-z ]
- Frizzled-4 (60 kDa, Protein from Homo sapiens)
- Gpcr complex (450 kDa, Complex from Homo sapiens)
- Segment polarity protein dishevelled homolog dvl-2 (79 kDa, Protein from Homo sapiens)
Gq bound FZD1 in ligand-free state
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8j9n
Q-score: 0.386
Zhang Z, Lin X, Wei L, Wu Y, Xu L, Wu L, Wei X, Zhao S, Zhu X, Xu F
Cell Discov (2024) 10 pp. 3-3 [ Pubmed: 38182578 DOI: doi:10.1038/s41421-023-00627-y ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,guanine nucleotide-binding protein g(q) subunit alpha (28 kDa, Protein from Homo sapiens)
- Nanobody nb35 (16 kDa, Protein from Vicugna pacos)
- Fzd1-gq complex in the ligand-free state (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Frizzled-1 (64 kDa, Protein from Homo sapiens)
Cryo-EM structure of inactive FZD1
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8j9o
Q-score: 0.347
Zhang Z, Lin X, Wei L, Wu Y, Xu L, Wu L, Wei X, Zhao S, Zhu X, Xu F
Cell Discov (2024) 10 pp. 3-3 [ Pubmed: 38182578 DOI: doi:10.1038/s41421-023-00627-y ]
- Frizzled-1,soluble cytochrome b562 (75 kDa, Protein from Homo sapiens)
- Anti-fab nanobody (13 kDa, Protein from Vicugna pacos)
- Anti-bril fab heavy chain (28 kDa, Protein from Mus)
- Frizzled-1 with icl3-bril complex with anti-bril fab (Complex from Homo sapiens)
- Anti-bril fab light chain (24 kDa, Protein from Mus)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8jh7
Q-score: 0.354
Zhang Z, Lin X, Wei L, Wu Y, Xu L, Wu L, Wei X, Zhao S, Zhu X, Xu F
Cell Discov (2024) 10 pp. 3-3 [ Pubmed: 38182578 DOI: doi:10.1038/s41421-023-00627-y ]
- Anti-bril fab heavy chain (25 kDa, Protein from Homo sapiens)
- Complex of human fzd6 in inactivate state with accessory proteins. (Complex from Homo sapiens)
- Anti-fab nanobody (14 kDa, Protein from synthetic construct)
- Anti-bril fab light chain (23 kDa, Protein from Homo sapiens)
- Frizzled-6,soluble cytochrome b562 (73 kDa, Protein from Homo sapiens)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8jhb
Q-score: 0.334
Zhang Z, Lin X, Wei L, Wu Y, Xu L, Wu L, Wei X, Zhao S, Zhu X, Xu F
Cell Discov (2024) 10 pp. 3-3 [ Pubmed: 38182578 DOI: doi:10.1038/s41421-023-00627-y ]
- Frizzled-6 (61 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (8 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Fzd6-gs complex with nb35. (Complex from Homo sapiens)
- Nb35 (14 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms xlas (31 kDa, Protein from Homo sapiens)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8jhc
Q-score: 0.387
Zhang Z, Lin X, Wei L, Wu Y, Xu L, Wu L, Wei X, Zhao S, Zhu X, Xu F
Cell Discov (2024) 10 pp. 3-3 [ Pubmed: 38182578 DOI: doi:10.1038/s41421-023-00627-y ]
- Anti-fab nanobody (14 kDa, Protein from synthetic construct)
- Frizzled-3,soluble cytochrome b562 (74 kDa, Protein from Homo sapiens)
- Anti-bril fab heavy chain (25 kDa, Protein from Homo sapiens)
- Complex of fzd3 with anti-bril fab and anti-fab nanobody (Complex from Homo sapiens)
- Anti-bril fab light chain (23 kDa, Protein from Homo sapiens)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8jhi
Q-score: 0.423
Zhang Z, Lin X, Wei L, Wu Y, Xu L, Wu L, Wei X, Zhao S, Zhu X, Xu F
Cell Discov (2024) 10 pp. 3-3 [ Pubmed: 38182578 DOI: doi:10.1038/s41421-023-00627-y ]
- Nb35 (13 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Complex of fzd3-gs heterotrimer with nb35. (Complex from Homo sapiens)
- Frizzled-3 (60 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms xlas (28 kDa, Protein from Homo sapiens)