Cryo-EM structure of ACh-bound M2R-Go signaling complex in S1 state
Resolution: 3.21 Å
EM Method: Single-particle
Fitted PDBs: 7t8x
Q-score: 0.517
Xu J, Wang Q, Hubner H, Hu Y, Niu X, Wang H, Maeda S, Inoue A, Tao Y, Gmeiner P, Du Y, Jin C, Kobilka BK
Nat Commun (2023) 14 pp. 376-376 [ DOI: doi:10.1038/s41467-022-35726-z Pubmed: 36690613 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Muscarinic acetylcholine receptor m2,muscarinic acetylcholine receptor m2 chimera (39 kDa, Protein from Homo sapiens)
- Acetylcholine (146 Da, Ligand)
- Antibody fragment (27 kDa, Protein from Mus musculus)
- Ach-bound m2r-goa-scfv16 complex (Complex from Homo sapiens)
Cryo-EM structure of ACh-bound M2R-Go signaling complex in S2 state
Resolution: 3.32 Å
EM Method: Single-particle
Fitted PDBs: 7t90
Q-score: 0.47
Xu J, Wang Q, Hubner H, Hu Y, Niu X, Wang H, Maeda S, Inoue A, Tao Y, Gmeiner P, Du Y, Jin C, Kobilka BK
Nat Commun (2023) 14 pp. 376-376 [ DOI: doi:10.1038/s41467-022-35726-z Pubmed: 36690613 ]
- Muscarinic acetylcholine receptor m2 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Acetylcholine (146 Da, Ligand)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Ach-bound m2r-goa-scfv16 complex (Complex from Homo sapiens)
Cryo-EM structure of S1 state ACh-bound M2R-Go signaling complex with a PAM
Resolution: 3.16 Å
EM Method: Single-particle
Fitted PDBs: 7t94
Q-score: 0.46
Xu J, Wang Q, Hubner H, Hu Y, Niu X, Wang H, Maeda S, Inoue A, Tao Y, Gmeiner P, Du Y, Jin C, Kobilka BK
Nat Commun (2023) 14 pp. 376-376 [ DOI: doi:10.1038/s41467-022-35726-z Pubmed: 36690613 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Muscarinic acetylcholine receptor m2,muscarinic acetylcholine receptor m2 chimera (39 kDa, Protein from Homo sapiens)
- Acetylcholine (146 Da, Ligand)
- Antibody fragment (27 kDa, Protein from Mus musculus)
- Ach-bound m2r-goa-scfv16 complex (Complex from Homo sapiens)
- 3-amino-5-chloro-n-cyclopropyl-4-methyl-6-[2-(4-methylpiperazin-1-yl)-2-oxoethoxy]thieno[2,3-b]pyridine-2-carboxamide (437 Da, Ligand)
Cryo-EM structure of S2 state ACh-bound M2R-Go signaling complex with a PAM
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 7t96
Q-score: 0.466
Xu J, Wang Q, Hubner H, Hu Y, Niu X, Wang H, Maeda S, Inoue A, Tao Y, Gmeiner P, Du Y, Jin C, Kobilka BK
Nat Commun (2023) 14 pp. 376-376 [ DOI: doi:10.1038/s41467-022-35726-z Pubmed: 36690613 ]
- Muscarinic acetylcholine receptor m2 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Acetylcholine (146 Da, Ligand)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Ach-bound m2r-goa-scfv16 complex (Complex from Homo sapiens)
- 3-amino-5-chloro-n-cyclopropyl-4-methyl-6-[2-(4-methylpiperazin-1-yl)-2-oxoethoxy]thieno[2,3-b]pyridine-2-carboxamide (437 Da, Ligand)
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 7w6p
Q-score: 0.056
Fink EA, Xu J, Hubner H, Braz JM, Seemann P, Avet C, Craik V, Weikert D, Schmidt MF, Webb CM, Tolmachova NA, Moroz YS, Huang XP, Kalyanaraman C, Gahbauer S, Chen G, Liu Z, Jacobson MP, Irwin JJ, Bouvier M, Du Y, Shoichet BK, Basbaum AI, Gmeiner P
Science (2022) 377 pp. eabn7065-eabn7065 [ DOI: doi:10.1126/science.abn7065 Pubmed: 36173843 ]
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Signaling complex of alpha2a adrenergic receptor with goa (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Alpha-2a adrenergic receptor (50 kDa, Protein from Homo sapiens)
- Scfv (32 kDa, Protein from Mus musculus)
- N-pyridin-4-ylisoquinolin-4-amine (221 Da, Ligand)
Cryo-EM structure of the alpha2A adrenergic receptor GoA signaling complex bound to a biased agonist
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7w7e
Q-score: 0.466
Fink EA, Xu J, Hubner H, Braz JM, Seemann P, Avet C, Craik V, Weikert D, Schmidt MF, Webb CM, Tolmachova NA, Moroz YS, Huang XP, Kalyanaraman C, Gahbauer S, Chen G, Liu Z, Jacobson MP, Irwin JJ, Bouvier M, Du Y, Shoichet BK, Basbaum AI, Gmeiner P
Science (2022) 377 pp. eabn7065-eabn7065 [ DOI: doi:10.1126/science.abn7065 Pubmed: 36173843 ]
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Signaling complex of alpha2a adrenergic receptor with goa (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Alpha-2a adrenergic receptor (50 kDa, Protein from Homo sapiens)
- 5-(3-bicyclo[4.2.0]octa-1,3,5-trienyl)-1,2,3,6-tetrahydropyridine (185 Da, Ligand)
- Scfv (32 kDa, Protein from Mus musculus)
Structure of the alpha2A-adrenergic receptor GoA signaling complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7ej0
Q-score: 0.426
Xu J, Cao S, Hubner H, Weikert D, Chen G, Lu Q, Yuan D, Gmeiner P, Liu Z, Du Y
Sci Adv (2022) 8 pp. eabj5347-eabj5347 [ DOI: doi:10.1126/sciadv.abj5347 Pubmed: 35245122 ]
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Scfv16 (32 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Alpha2a-adrenergic receptor go signaling complex (Complex from Homo sapiens)
- Alpha-2a adrenergic receptor (50 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Mus musculus)
- Noradrenaline (170 Da, Ligand)
- Alpha2a-adrenergic receptor go signaling complex (Complex)
Structure of the alpha2A-adrenergic receptor GoA signaling complex bound to brimonidine
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7ej8
Q-score: 0.482
Xu J, Cao S, Hubner H, Weikert D, Chen G, Lu Q, Yuan D, Gmeiner P, Liu Z, Du Y
Sci Adv (2022) 8 pp. eabj5347-eabj5347 [ DOI: doi:10.1126/sciadv.abj5347 Pubmed: 35245122 ]
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Scfv16 (32 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Alpha2a-adrenergic receptor go signaling complex (Complex from Homo sapiens)
- Alpha-2a adrenergic receptor (50 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Mus musculus)
- Alpha2a-adrenergic receptor go signaling complex (Complex)
- Brimonidine (292 Da, Ligand)
Structure of the alpha2A-adrenergic receptor GoA signaling complex bound to dexmedetomidine
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7eja
Q-score: 0.378
Xu J, Cao S, Hubner H, Weikert D, Chen G, Lu Q, Yuan D, Gmeiner P, Liu Z, Du Y
Sci Adv (2022) 8 pp. eabj5347-eabj5347 [ DOI: doi:10.1126/sciadv.abj5347 Pubmed: 35245122 ]
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Scfv16 (32 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Alpha2a-adrenergic receptor go signaling complex (Complex from Homo sapiens)
- Alpha-2a adrenergic receptor (50 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Mus musculus)
- 4-[(1~{s})-1-(2,3-dimethylphenyl)ethyl]-1~{h}-imidazole (200 Da, Ligand)
- Alpha2a-adrenergic receptor go signaling complex (Complex)
Structure of the alpha2A-adrenergic receptor GoA signaling complex bound to oxymetazoline
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7ejk
Q-score: 0.453
Xu J, Cao S, Hubner H, Weikert D, Chen G, Lu Q, Yuan D, Gmeiner P, Liu Z, Du Y
Sci Adv (2022) 8 pp. eabj5347-eabj5347 [ DOI: doi:10.1126/sciadv.abj5347 Pubmed: 35245122 ]
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Scfv16 (32 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Alpha2a-adrenergic receptor go signaling complex (Complex from Homo sapiens)
- Alpha-2a adrenergic receptor (50 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Mus musculus)
- Oxymetazoline (260 Da, Ligand)
- Alpha2a-adrenergic receptor go signaling complex (Complex)