Cryo-EM structure of ACh-bound M2R-Go signaling complex in S1 state
Resolution: 3.21 Å
EM Method: Single-particle
Fitted PDBs: 7t8x
Q-score: 0.517
Xu J, Wang Q, Hubner H, Hu Y, Niu X, Wang H, Maeda S, Inoue A, Tao Y, Gmeiner P, Du Y, Jin C, Kobilka BK
Nat Commun (2023) 14 pp. 376-376 [ DOI: doi:10.1038/s41467-022-35726-z Pubmed: 36690613 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Muscarinic acetylcholine receptor m2,muscarinic acetylcholine receptor m2 chimera (39 kDa, Protein from Homo sapiens)
- Acetylcholine (146 Da, Ligand)
- Antibody fragment (27 kDa, Protein from Mus musculus)
- Ach-bound m2r-goa-scfv16 complex (Complex from Homo sapiens)
Cryo-EM structure of ACh-bound M2R-Go signaling complex in S2 state
Resolution: 3.32 Å
EM Method: Single-particle
Fitted PDBs: 7t90
Q-score: 0.47
Xu J, Wang Q, Hubner H, Hu Y, Niu X, Wang H, Maeda S, Inoue A, Tao Y, Gmeiner P, Du Y, Jin C, Kobilka BK
Nat Commun (2023) 14 pp. 376-376 [ DOI: doi:10.1038/s41467-022-35726-z Pubmed: 36690613 ]
- Muscarinic acetylcholine receptor m2 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Acetylcholine (146 Da, Ligand)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Ach-bound m2r-goa-scfv16 complex (Complex from Homo sapiens)
Cryo-EM structure of S1 state ACh-bound M2R-Go signaling complex with a PAM
Resolution: 3.16 Å
EM Method: Single-particle
Fitted PDBs: 7t94
Q-score: 0.46
Xu J, Wang Q, Hubner H, Hu Y, Niu X, Wang H, Maeda S, Inoue A, Tao Y, Gmeiner P, Du Y, Jin C, Kobilka BK
Nat Commun (2023) 14 pp. 376-376 [ DOI: doi:10.1038/s41467-022-35726-z Pubmed: 36690613 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Muscarinic acetylcholine receptor m2,muscarinic acetylcholine receptor m2 chimera (39 kDa, Protein from Homo sapiens)
- Acetylcholine (146 Da, Ligand)
- Antibody fragment (27 kDa, Protein from Mus musculus)
- Ach-bound m2r-goa-scfv16 complex (Complex from Homo sapiens)
- 3-amino-5-chloro-n-cyclopropyl-4-methyl-6-[2-(4-methylpiperazin-1-yl)-2-oxoethoxy]thieno[2,3-b]pyridine-2-carboxamide (437 Da, Ligand)
Cryo-EM structure of S2 state ACh-bound M2R-Go signaling complex with a PAM
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 7t96
Q-score: 0.466
Xu J, Wang Q, Hubner H, Hu Y, Niu X, Wang H, Maeda S, Inoue A, Tao Y, Gmeiner P, Du Y, Jin C, Kobilka BK
Nat Commun (2023) 14 pp. 376-376 [ DOI: doi:10.1038/s41467-022-35726-z Pubmed: 36690613 ]
- Muscarinic acetylcholine receptor m2 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Acetylcholine (146 Da, Ligand)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Ach-bound m2r-goa-scfv16 complex (Complex from Homo sapiens)
- 3-amino-5-chloro-n-cyclopropyl-4-methyl-6-[2-(4-methylpiperazin-1-yl)-2-oxoethoxy]thieno[2,3-b]pyridine-2-carboxamide (437 Da, Ligand)