PI 3-kinase alpha with nanobody 3-126
Resolution: 2.41 Å
EM Method: Single-particle
Fitted PDBs: 8dcp
Q-score: 0.584
Hart JR, Liu X, Pan C, Liang A, Ueno L, Xu Y, Quezada A, Zou X, Yang S, Zhou Q, Schoonooghe S, Hassanzadeh-Ghassabeh G, Xia T, Shui W, Yang D, Vogt PK, Wang MW
PNAS (2022) 119 pp. e2210769119-e2210769119 [ Pubmed: 36095215 DOI: doi:10.1073/pnas.2210769119 ]
- Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (127 kDa, Protein from Homo sapiens)
- Human pi 3-kinase alpha complex composed of p110alpha and p85alpha with nanobody 3-126 bound (Complex from Homo sapiens)
- Phosphatidylinositol 3-kinase regulatory subunit alpha (83 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
PI 3-kinase alpha with nanobody 3-159
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8dcx
Q-score: 0.345
Hart JR, Liu X, Pan C, Liang A, Ueno L, Xu Y, Quezada A, Zou X, Yang S, Zhou Q, Schoonooghe S, Hassanzadeh-Ghassabeh G, Xia T, Shui W, Yang D, Vogt PK, Wang MW
PNAS (2022) 119 pp. e2210769119-e2210769119 [ DOI: doi:10.1073/pnas.2210769119 Pubmed: 36095215 ]
- Phosphatidylinositol 3-kinase regulatory subunit alpha (83 kDa, Protein from Homo sapiens)
- Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (127 kDa, Protein from Homo sapiens)
- Human pi 3-kinase alpha complex composed of p110alpha and p85alpha with nanobody 3-159 bound (Complex from Homo sapiens)
PI 3-kinase alpha with nanobody 3-142
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8dd4
Q-score: 0.42
Hart JR, Liu X, Pan C, Liang A, Ueno L, Xu Y, Quezada A, Zou X, Yang S, Zhou Q, Schoonooghe S, Hassanzadeh-Ghassabeh G, Xia T, Shui W, Yang D, Vogt PK, Wang MW
PNAS (2022) 119 pp. e2210769119-e2210769119 [ Pubmed: 36095215 DOI: doi:10.1073/pnas.2210769119 ]
- Human pi 3-kinase alpha complex composed of p110alpha and p85alpha with nanobody 3-142 bound (Complex from Homo sapiens)
- Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (127 kDa, Protein from Homo sapiens)
- Phosphatidylinositol 3-kinase regulatory subunit alpha (83 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
PI 3-kinase alpha with nanobody 3-142, crosslinked with DSG
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8dd8
Q-score: 0.454
Hart JR, Liu X, Pan C, Liang A, Ueno L, Xu Y, Quezada A, Zou X, Yang S, Zhou Q, Schoonooghe S, Hassanzadeh-Ghassabeh G, Xia T, Shui W, Yang D, Vogt PK, Wang MW
PNAS (2022) 119 pp. e2210769119-e2210769119 [ DOI: doi:10.1073/pnas.2210769119 Pubmed: 36095215 ]
- Phosphatidylinositol 3-kinase regulatory subunit alpha (83 kDa, Protein from Homo sapiens)
- Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (127 kDa, Protein from Homo sapiens)
- Human pi 3-kinase alpha complex composed of p110alpha and p85alpha with nanobody 3-142 bound (Complex from Homo sapiens)
Human vasoactive intestinal polypeptide receptor 2 (VIP2R) in complex with PACAP27 and Gs
Resolution: 2.74 Å
EM Method: Single-particle
Fitted PDBs: 7vqx
Q-score: 0.543
Xu Y, Feng W, Zhou Q, Liang A, Li J, Dai A, Zhao F, Yan J, Chen CW, Li H, Zhao LH, Xia T, Jiang Y, Xu HE, Yang D, Wang MW
Nat Commun (2022) 13 pp. 2272-2272 [ DOI: doi:10.1038/s41467-022-30041-z Pubmed: 35477937 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Bos taurus)
- Nanobody-35 (15 kDa, Protein from synthetic construct)
- Vasoactive intestinal polypeptide receptor 2 (64 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Pituitary adenylate cyclase-activating polypeptide 27 (3 kDa, Protein from Homo sapiens)
- Nanobody-35 (Complex)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex)
- Human vasoactive intestinal polypeptide receptor 2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) /g(s) /g(o) subunit gamma-2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (43 kDa, Protein from Rattus norvegicus)
- Cryo-em structure of the human vasoactive intestinal polypeptide receptor 2 in commplex with pacap27 and g protein (Complex from Bos taurus)
- Guanine nucleotide-binding protein g(i) /g(s) /g(t) subunit beta-1 (Complex from synthetic construct)
- Pituitary adenylate cyclase activating peptide-27 (Complex from Bos taurus)
Resolution: 3.42 Å
EM Method: Single-particle
Fitted PDBs: 7wbj
Q-score: 0.425
Xu Y, Feng W, Zhou Q, Liang A, Li J, Dai A, Zhao F, Yan J, Chen CW, Li H, Zhao LH, Xia T, Jiang Y, Xu HE, Yang D, Wang MW
Nat Commun (2022) 13 pp. 2272-2272 [ DOI: doi:10.1038/s41467-022-30041-z Pubmed: 35477937 ]
- Vasoactive intestinal polypeptide receptor 2 (49 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from synthetic construct)
- Nanobody-35 (15 kDa, Protein from synthetic construct)
- Pituitary adenylate cyclase-activating polypeptide 27 (Complex from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Cryo-em structure of the human vasoactive intestinal polypeptide receptor 2 in commplex with pacap27 and g protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (43 kDa, Protein from Rattus norvegicus)
- Vasoactive intestinal polypeptide receptor 2 (Complex)
- Pituitary adenylate cyclase-activating polypeptide 27 (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short/g(i)/g(s)/g(o) subunit gamma-2 (Complex from Rattus norvegicus)
- Nanobody-35 (Complex)
Cryo-EM Structure of p110alpha in complex with p85alpha
Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 7myn
Q-score: 0.472
Liu X, Yang S, Hart JR, Xu Y, Zou X, Zhang H, Zhou Q, Xia T, Zhang Y, Yang D, Wang MW, Vogt PK
PNAS (2021) 118 [ Pubmed: 34725156 DOI: doi:10.1073/pnas.2109327118 ]
- Heterodimer of pik3ca with pik3r1 (200 kDa, Complex from Homo sapiens)
- Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (127 kDa, Protein from Homo sapiens)
- Phosphatidylinositol 3-kinase regulatory subunit alpha (83 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
Cryo-EM structure of p110alpha in complex with p85alpha inhibited by BYL-719
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 7myo
Q-score: 0.38
Liu X, Yang S, Hart JR, Xu Y, Zou X, Zhang H, Zhou Q, Xia T, Zhang Y, Yang D, Wang MW, Vogt PK
PNAS (2021) 118 [ Pubmed: 34725156 DOI: doi:10.1073/pnas.2109327118 ]
- Heterodimer of p110alpha with p85alpha (200 kDa, Complex from Homo sapiens)
- Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (127 kDa, Protein from Homo sapiens)
- Phosphatidylinositol 3-kinase regulatory subunit alpha (83 kDa, Protein from Homo sapiens)
- (2s)-n~1~-{4-methyl-5-[2-(1,1,1-trifluoro-2-methylpropan-2-yl)pyridin-4-yl]-1,3-thiazol-2-yl}pyrrolidine-1,2-dicarboxamide (441 Da, Ligand)