Cryo-EM structure of the alpha-MSH-bound human melanocortin receptor 5 (MC5R)-Gs complex
Resolution: 2.73 Å
EM Method: Single-particle
Fitted PDBs: 8inr
Q-score: 0.49
Feng W, Zhou Q, Chen X, Dai A, Cai X, Liu X, Zhao F, Chen Y, Ye C, Xu Y, Cong Z, Li H, Lin S, Yang D, Wang MW
Cell Discov (2023) 9 pp. 81- [ Pubmed: 37524700 DOI: doi:10.1107/S0907444909042073 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1,hibit (40 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Ha signal peptide,melanocortin receptor 5,lgbit subunit (56 kDa, Protein from Homo sapiens)
- Alpha-melanocyte-stimulating hormone (1 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Cryo-em structure of alpha-msh-bound human melanocortin receptor 5 in complex with g protein (Complex from Homo sapiens)
Cryo-EM structure of the gamma-MSH-bound human melanocortin receptor 3 (MC3R)-Gs complex
Resolution: 2.86 Å
EM Method: Single-particle
Fitted PDBs: 8ioc
Q-score: 0.459
Feng W, Zhou Q, Chen X, Dai A, Cai X, Liu X, Zhao F, Chen Y, Ye C, Xu Y, Cong Z, Li H, Lin S, Yang D, Wang MW
Cell Discov (2023) 9 pp. 81- [ Pubmed: 37524700 DOI: doi:10.1107/S0907444909042073 ]
- Gamma-melanocyte-stimulating hormone (1 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Ha signal peptide,melanocortin receptor 3,lgbit subunit (56 kDa, Protein from Homo sapiens)
- Cryo-em structure of gamma-msh-bound human melanocortin receptor 3 in complex with g protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Nanobody-35 (17 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1,hibit (40 kDa, Protein from Homo sapiens)
Cryo-EM structure of the PG-901-bound human melanocortin receptor 5 (MC5R)-Gs complex
Resolution: 2.59 Å
EM Method: Single-particle
Fitted PDBs: 8iod
Q-score: 0.503
Feng W, Zhou Q, Chen X, Dai A, Cai X, Liu X, Zhao F, Chen Y, Ye C, Xu Y, Cong Z, Li H, Lin S, Yang D, Wang MW
Cell Discov (2023) 9 pp. 81- [ Pubmed: 37524700 DOI: doi:10.1107/S0907444909042073 ]
- Pg-901 (1 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1,hibit (40 kDa, Protein from Homo sapiens)
- Cryo-em structure of pg-901-bound human melanocortin receptor 5 in complex with g protein (Complex from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Ha signal peptide,melanocortin receptor 5,lgbit subunit (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Nanobody-35 (17 kDa, Protein from Homo sapiens)
Cryo-EM structure of cancer-specific PI3Kalpha mutant E542K in complex with BYL-719
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 8gua
Q-score: 0.484
Liu X, Zhou Q, Hart JR, Xu Y, Yang S, Yang D, Vogt PK, Wang MW
PNAS (2022) 119 pp. e2215621119-e2215621119 [ Pubmed: 36343266 DOI: doi:10.1073/pnas.2215621119 ]
- Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (127 kDa, Protein from Homo sapiens)
- (2s)-n~1~-{4-methyl-5-[2-(1,1,1-trifluoro-2-methylpropan-2-yl)pyridin-4-yl]-1,3-thiazol-2-yl}pyrrolidine-1,2-dicarboxamide (441 Da, Ligand)
- Human pi3kalpha mutant e542k in complex with byl-719 (Complex from Homo sapiens)
Cryo-EM structure of cancer-specific PI3Kalpha mutant H1047R in complex with BYL-719
Resolution: 2.73 Å
EM Method: Single-particle
Fitted PDBs: 8gub
Q-score: 0.531
Liu X, Zhou Q, Hart JR, Xu Y, Yang S, Yang D, Vogt PK, Wang MW
PNAS (2022) 119 pp. e2215621119-e2215621119 [ Pubmed: 36343266 DOI: doi:10.1073/pnas.2215621119 ]
- Human pi3kalpha mutant h1047r in complex with byl-719 (Complex from Homo sapiens)
- (2s)-n~1~-{4-methyl-5-[2-(1,1,1-trifluoro-2-methylpropan-2-yl)pyridin-4-yl]-1,3-thiazol-2-yl}pyrrolidine-1,2-dicarboxamide (441 Da, Ligand)
- Phosphatidylinositol 3-kinase regulatory subunit alpha (83 kDa, Protein from Homo sapiens)
- Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (127 kDa, Protein from Homo sapiens)
Cryo-EM structure of cancer-specific PI3Kalpha mutant E545K in complex with BYL-719
Resolution: 2.62 Å
EM Method: Single-particle
Fitted PDBs: 8gud
Q-score: 0.44
Liu X, Zhou Q, Hart JR, Xu Y, Yang S, Yang D, Vogt PK, Wang MW
PNAS (2022) 119 pp. e2215621119-e2215621119 [ Pubmed: 36343266 DOI: doi:10.1073/pnas.2215621119 ]
- (2s)-n~1~-{4-methyl-5-[2-(1,1,1-trifluoro-2-methylpropan-2-yl)pyridin-4-yl]-1,3-thiazol-2-yl}pyrrolidine-1,2-dicarboxamide (441 Da, Ligand)
- Cryo-em structure of pi3kalpha mutant e545k in complex with byl-719 (Complex from Homo sapiens)
- Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (127 kDa, Protein from Homo sapiens)
PI 3-kinase alpha with nanobody 3-126
Resolution: 2.41 Å
EM Method: Single-particle
Fitted PDBs: 8dcp
Q-score: 0.584
Hart JR, Liu X, Pan C, Liang A, Ueno L, Xu Y, Quezada A, Zou X, Yang S, Zhou Q, Schoonooghe S, Hassanzadeh-Ghassabeh G, Xia T, Shui W, Yang D, Vogt PK, Wang MW
PNAS (2022) 119 pp. e2210769119-e2210769119 [ Pubmed: 36095215 DOI: doi:10.1073/pnas.2210769119 ]
- Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (127 kDa, Protein from Homo sapiens)
- Human pi 3-kinase alpha complex composed of p110alpha and p85alpha with nanobody 3-126 bound (Complex from Homo sapiens)
- Phosphatidylinositol 3-kinase regulatory subunit alpha (83 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
PI 3-kinase alpha with nanobody 3-142
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8dd4
Q-score: 0.42
Hart JR, Liu X, Pan C, Liang A, Ueno L, Xu Y, Quezada A, Zou X, Yang S, Zhou Q, Schoonooghe S, Hassanzadeh-Ghassabeh G, Xia T, Shui W, Yang D, Vogt PK, Wang MW
PNAS (2022) 119 pp. e2210769119-e2210769119 [ Pubmed: 36095215 DOI: doi:10.1073/pnas.2210769119 ]
- Human pi 3-kinase alpha complex composed of p110alpha and p85alpha with nanobody 3-142 bound (Complex from Homo sapiens)
- Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (127 kDa, Protein from Homo sapiens)
- Phosphatidylinositol 3-kinase regulatory subunit alpha (83 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
Cryo-EM Structure of p110alpha in complex with p85alpha
Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 7myn
Q-score: 0.472
Liu X, Yang S, Hart JR, Xu Y, Zou X, Zhang H, Zhou Q, Xia T, Zhang Y, Yang D, Wang MW, Vogt PK
PNAS (2021) 118 [ Pubmed: 34725156 DOI: doi:10.1073/pnas.2109327118 ]
- Heterodimer of pik3ca with pik3r1 (200 kDa, Complex from Homo sapiens)
- Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (127 kDa, Protein from Homo sapiens)
- Phosphatidylinositol 3-kinase regulatory subunit alpha (83 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
Cryo-EM structure of p110alpha in complex with p85alpha inhibited by BYL-719
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 7myo
Q-score: 0.38
Liu X, Yang S, Hart JR, Xu Y, Zou X, Zhang H, Zhou Q, Xia T, Zhang Y, Yang D, Wang MW, Vogt PK
PNAS (2021) 118 [ Pubmed: 34725156 DOI: doi:10.1073/pnas.2109327118 ]
- Heterodimer of p110alpha with p85alpha (200 kDa, Complex from Homo sapiens)
- Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (127 kDa, Protein from Homo sapiens)
- Phosphatidylinositol 3-kinase regulatory subunit alpha (83 kDa, Protein from Homo sapiens)
- (2s)-n~1~-{4-methyl-5-[2-(1,1,1-trifluoro-2-methylpropan-2-yl)pyridin-4-yl]-1,3-thiazol-2-yl}pyrrolidine-1,2-dicarboxamide (441 Da, Ligand)