Structure of Lassa virus polymerase bound to Z matrix protein
Resolution: 3.88 Å
EM Method: Single-particle
Fitted PDBs: 7ckl
Q-score: 0.343
Xu X, Peng R, Peng Q, Wang M, Xu Y, Liu S, Tian X, Deng H, Tong Y, Hu X, Zhong J, Wang P, Qi J, Gao GF, Shi Y
Nat Microbiol (2021) 6 pp. 921-931 [ DOI: doi:10.1038/s41564-021-00916-w ]
- Ring finger protein z (10 kDa, Protein from Lassa mammarenavirus)
- Lassa virus polymerase in complex with z matrix protein (260 kDa, Complex from Lassa mammarenavirus)
- Manganese (ii) ion (54 Da, Ligand)
- Rna-directed rna polymerase l (253 kDa, Protein from Lassa mammarenavirus)
- Zinc ion (65 Da, Ligand)
Structure of Machupo virus polymerase bound to Z matrix protein (monomeric complex)
Resolution: 3.37 Å
EM Method: Single-particle
Fitted PDBs: 7ckm
Q-score: 0.491
Xu X, Peng R, Peng Q, Wang M, Xu Y, Liu S, Tian X, Deng H, Tong Y, Hu X, Zhong J, Wang P, Qi J, Gao GF, Shi Y
Nat Microbiol (2021) 6 pp. 921-931 [ DOI: doi:10.1038/s41564-021-00916-w ]
- Ring finger protein z (10 kDa, Protein from Machupo virus)
- Rna-directed rna polymerase l (250 kDa, Protein from Machupo virus)
- Manganese (ii) ion (54 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Machupo virus polymerase in complex with z matrix protein (260 kDa, Complex from Machupo mammarenavirus)
Structure of Machupo virus L polymerase in complex with Z protein (dimeric form)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7elb
Q-score: 0.229
Xu X, Peng R, Peng Q, Wang M, Xu Y, Liu S, Tian X, Deng H, Tong Y, Hu X, Zhong J, Wang P, Qi J, Gao GF, Shi Y
Nat Microbiol (2021) 6 pp. 921-931 [ DOI: doi:10.1038/s41564-021-00916-w Pubmed: 34127846 ]
- Rna-directed rna polymerase l (250 kDa, Protein from Machupo mammarenavirus)
- Machupo virus polymerase in complex with z protein (dimeric form). (580 kDa, Complex from Machupo mammarenavirus)
- Ring finger protein z (10 kDa, Protein from Machupo mammarenavirus)
- Machupo virus polymerase (Complex from Machupo mammarenavirus)
- Manganese (ii) ion (54 Da, Ligand)
- Machupo virus z matrix protein (Complex)
- Zinc ion (65 Da, Ligand)
Structure of LbCas12a-crRNA complex bound to AcrVA4 (form A complex)
Resolution: 3.25 Å
EM Method: Single-particle
Fitted PDBs: 6kl9
Q-score: 0.434
Peng R, Li Z, Xu Y, He S, Peng Q, Wu LA, Wu Y, Qi J, Wang P, Shi Y, Gao GF
PNAS (2019) 116 pp. 18928-18936 [ Pubmed: 31467167 DOI: doi:10.1073/pnas.1909400116 ]
- Lbcas12a (143 kDa, Protein from Lachnospiraceae bacterium)
- Water (18 Da, Ligand)
- Rna (42-mer) (13 kDa, RNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Lbcas12a-crrna complex bound to acrva4 (form a complex) (170 kDa, Complex from Lachnospiraceae bacterium)
- Acrva4 (27 kDa, Protein from Moraxella bovoculi)
Structure of LbCas12a-crRNA complex bound to AcrVA4 (form B complex)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6klb
Q-score: 0.224
Peng R, Li Z, Xu Y, He S, Peng Q, Wu LA, Wu Y, Qi J, Wang P, Shi Y, Gao GF
PNAS (2019) 116 pp. 18928-18936 [ Pubmed: 31467167 DOI: doi:10.1073/pnas.1909400116 ]
- Lbcas12a (143 kDa, Protein from Lachnospiraceae bacterium)
- Water (18 Da, Ligand)
- Lbcas12a-crrna complex bound to acrva4 (form b complex) (300 kDa, Complex from Lachnospiraceae bacterium)
- Magnesium ion (24 Da, Ligand)
- Acrva4 (27 kDa, Protein from Moraxella bovoculi)
- Crrna (13 kDa, RNA from synthetic construct)
Anti-CRISPR protein AcrF1/2 bound to Csy surveillance complex with a 20nt spacer crRNA
Peng R, Xu Y, Zhu T, Li N, Qi J, Chai Y, Wu M, Zhang X, Shi Y, Wang P, Wang J, Gao N, Gao GF
Cell Res (2017) 27 pp. 853-864 [ DOI: doi:10.1038/cr.2017.79 Pubmed: 28574055 ]
- Csy2 (Protein from Pseudomonas aeruginosa)
- Anti-crispr proteins acrf1/2 bound to csy surveillance complex with a 20nt spacer crrna (300 kDa, Complex from Pseudomonas aeruginosa)
- Csy1 (Protein from Pseudomonas aeruginosa)
- Csy4 (Protein from Pseudomonas aeruginosa)
- Csy3 (Protein from Pseudomonas aeruginosa)
- Acrf1 (Protein from Pseudomonas phage D3)
- Crrna with 20nt spacer (RNA from Pseudomonas aeruginosa)
- Acrf2 (Protein from Pseudomonas phage 73)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 5xlo
Q-score: 0.337
Peng R, Xu Y, Zhu T, Li N, Qi J, Chai Y, Wu M, Zhang X, Shi Y, Wang P, Wang J, Gao N, Gao GF
Cell Res (2017) 27 pp. 853-864 [ DOI: doi:10.1038/cr.2017.79 Pubmed: 28574055 ]
- Uncharacterized protein acrf1 (8 kDa, Protein from Pseudomonas phage JBD30)
- Anti-crispr proteins acrf1/2 bound to csy surveillance complex with a 32nt spacer crrna backbone region (400 kDa, Complex from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Crrna with 32nt spacer sequence (19 kDa, RNA from Pseudomonas aeruginosa)
- Crispr-associated protein csy3 (Complex)
- Acrf1 (Complex)
- Crrna with 20nt spacer sequence (Complex)
- Crispr-associated protein csy3 (37 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
Anti-CRISPR protein AcrF1 bound to Csy surveillance complex with 32nt spacer crRNA in binding mode B
Peng R, Xu Y, Zhu T, Li N, Qi J, Chai Y, Wu M, Zhang X, Shi Y, Wang P, Wang J, Gao N, Gao GF
Cell Res (2017) 27 pp. 853-864 [ DOI: doi:10.1038/cr.2017.79 Pubmed: 28574055 ]
- Crrna with 32nt spacer (RNA from Pseudomonas aeruginosa)
- Csy2 (Protein from Pseudomonas aeruginosa)
- Csy1 (Protein from Pseudomonas aeruginosa)
- Csy4 (Protein from Pseudomonas aeruginosa)
- Csy3 (Protein from Pseudomonas aeruginosa)
- Acrf1 (Protein from Pseudomonas aeruginosa)
- Anti-crispr protein acrf1 bound to csy surveillance complex with 32nt spacer crrna in binding mode b (400 kDa, Complex from Pseudomonas aeruginosa)
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 5xlp
Q-score: 0.26
Peng R, Xu Y, Zhu T, Li N, Qi J, Chai Y, Wu M, Zhang X, Shi Y, Wang P, Wang J, Gao N, Gao GF
Cell Res (2017) 27 pp. 853-864 [ DOI: doi:10.1038/cr.2017.79 Pubmed: 28574055 ]
- Crrna with 20nt spacer sequence (15 kDa, RNA from Pseudomonas aeruginosa)
- Crispr-associated protein csy3 (37 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Anti-crispr proteins acrf1/2 bound to csy surveillance complex with a 20nt spacer sequence backbone region (300 kDa, Complex from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Uncharacterized protein acrf1 (8 kDa, Protein from Pseudomonas phage JBD30)
- Man crispr-associated protein csy3 (Complex)
- Rna (Complex)
- Acrf1 (Complex)
Resolution: 9.0 Å
EM Method: Single-particle
Fitted PDBs: 3j3t
Q-score: 0.077
Liu J, Mei Z, Li N, Qi Y, Xu Y, Shi Y, Wang F, Lei J, Gao N
J Biol Chem (2013) 288 pp. 17597-17608 [ Pubmed: 23595989 DOI: doi:10.1074/jbc.M113.458752 ]
- Clpc (Protein from Bacillus subtilis)
- Meca-clpc(e280a) (600 kDa, Sample)
- Meca (Protein from Bacillus subtilis)