MPOX E5 hexamer AMP-PNP and ssDNA bound form with clear primase domain
Resolution: 2.91 Å
EM Method: Single-particle
Fitted PDBs: 8wgy
Q-score: 0.494
Xu Y, Wu Y, Zhang Y, Gao K, Wu X, Yang Y, Li D, Yang B, Zhang Z, Dong C
Sci Adv (2024) 10 pp. eadl1150-eadl1150 [ DOI: doi:10.1126/sciadv.adl1150 Pubmed: 39167653 ]
- Mpox helicase (Complex from Monkeypox virus)
- Uncoating factor opg117 (90 kDa, Protein from Monkeypox virus)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna (5'-d(p*cp*cp*c)-3') (822 Da, DNA from Spodoptera frugiperda)
- Zinc ion (65 Da, Ligand)
MPOX E5 double hexamer ssDNA bound conformation
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 8wgz
Q-score: 0.273
Xu Y, Wu Y, Zhang Y, Gao K, Wu X, Yang Y, Li D, Yang B, Zhang Z, Dong C
Sci Adv (2024) 10 pp. eadl1150-eadl1150 [ DOI: doi:10.1126/sciadv.adl1150 Pubmed: 39167653 ]
- Dna (5'-d(p*cp*cp*cp*cp*c)-3') (1 kDa, DNA from Spodoptera frugiperda)
- Mpox helicase (Complex from Monkeypox virus)
- Uncoating factor opg117 (90 kDa, Protein from Monkeypox virus)
Monkeypox virus DNA replication holoenzyme F8, A22 and E4 in complex with a DNA duplex and dCTP
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 8j8f
Q-score: 0.45
Xu Y, Wu Y, Wu X, Zhang Y, Yang Y, Li D, Yang B, Gao K, Zhang Z, Dong C
Int J Biol Macromol (2024) 270 pp. 132231-132231 [ Pubmed: 38735603 DOI: doi:10.1016/j.ijbiomac.2024.132231 ]
- 2'-deoxycytidine-5'-triphosphate (467 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- E4r (27 kDa, Protein from Monkeypox virus)
- Dna polymerase (120 kDa, Protein from Monkeypox virus)
- Monkeypox virus replication holoenzyme f8-a22-e4 in complex with a dna duplex and dctp (191 MDa, Complex from Monkeypox virus)
- Dna (5'-d(p*gp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*gp*ap*tp*ap*ap*cp*tp*tp*ap*ap*tp*cp*tp*cp*ap*cp*ap*tp*ap*gp*cp*ap*gp*cp*t)-3') (11 kDa, DNA from DNA molecule)
- Dna polymerase processivity factor component a20 (49 kDa, Protein from Monkeypox virus)
- Dna (5'-d(p*ap*gp*cp*tp*gp*cp*tp*ap*tp*gp*ap*gp*ap*tp*tp*ap*ap*gp*tp*tp*ap*t)-3') (7 kDa, DNA from DNA molecule)
Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 8j8g
Q-score: 0.471
Xu Y, Wu Y, Wu X, Zhang Y, Yang Y, Li D, Yang B, Gao K, Zhang Z, Dong C
Int J Biol Macromol (2024) 270 pp. 132231-132231 [ Pubmed: 38735603 DOI: doi:10.1016/j.ijbiomac.2024.132231 ]
- Monkeypox virus replication holoenzyme f8-a22-e4 in complex with dna duplex and cidofovir diphosphate (191 MDa, Complex from Monkeypox virus)
- Calcium ion (40 Da, Ligand)
- [(2s)-1-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3-oxidanyl-propan-2-yl]oxymethyl-[oxidanyl(phosphonooxy)phosphoryl]oxy-phosphinic acid (439 Da, Ligand)
- E4r (27 kDa, Protein from Monkeypox virus)
- Dna polymerase (120 kDa, Protein from Monkeypox virus)
- Dna (5'-d(p*gp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*gp*ap*tp*ap*ap*cp*tp*tp*ap*ap*tp*cp*tp*cp*ap*cp*ap*tp*ap*gp*cp*ap*gp*cp*t)-3') (11 kDa, DNA from DNA molecule)
- Dna polymerase processivity factor component a20 (49 kDa, Protein from Monkeypox virus)
- Dna (5'-d(p*ap*gp*cp*tp*gp*cp*tp*ap*tp*gp*ap*gp*ap*tp*tp*ap*ap*gp*tp*tp*ap*t)-3') (7 kDa, DNA from DNA molecule)
Monkeypox virus DNA replication holoenzyme F8, A22 and E4 complex in a DNA binding form
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 8j86
Q-score: 0.362
Xu Y, Wu Y, Wu X, Zhang Y, Yang Y, Li D, Yang B, Gao K, Zhang Z, Dong C
Int J Biol Macromol (2024) 270 pp. 132231-132231 [ Pubmed: 38735603 DOI: doi:10.1016/j.ijbiomac.2024.132231 ]
- Monkeypox virus replication holoenzyme f8-a22-e4 complex with dna duplex only (191 MDa, Complex from Monkeypox virus)
- Dna polymerase processivity factor component a20 (49 kDa, Protein from Monkeypox virus)
- Dna (5'-d(p*ap*gp*cp*tp*gp*cp*tp*ap*tp*gp*tp*gp*ap*gp*ap*tp*tp*ap*ap*gp*tp*tp*ap*t)-3') (7 kDa, DNA from DNA molecule)
- Calcium ion (40 Da, Ligand)
- Dna (5'-d(p*gp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*gp*ap*tp*ap*ap*cp*tp*tp*ap*ap*tp*cp*tp*cp*ap*cp*ap*tp*ap*gp*cp*ap*gp*cp*tp*)-3') (11 kDa, DNA from DNA molecule)
- E4r (27 kDa, Protein from Monkeypox virus)
- Dna polymerase (120 kDa, Protein from Monkeypox virus)
Monkeypox virus DNA replication holoenzyme F8, A22 and E4 complex in a DNA binding form
Resolution: 3.01 Å
EM Method: Single-particle
Fitted PDBs: 8hpa
Q-score: 0.374
Xu Y, Wu Y, Zhang Y, Fan R, Yang Y, Li D, Zhu S, Yang B, Zhang Z, Dong C
Cell Res (2023) 33 pp. 479-482 [ DOI: doi:10.1038/s41422-023-00796-1 Pubmed: 36973539 ]
- Dna (5'-d(*cp*gp*ap*tp*cp*cp*tp*tp*cp*cp*cp*cp*tp*ap*c)-3') (6 kDa, DNA from DNA molecule)
- Dna (5'-d(p*ap*tp*gp*gp*tp*ap*gp*gp*gp*gp*ap*ap*gp*gp*ap*tp*cp*g)-3') (8 kDa, DNA from DNA molecule)
- Monkeypox virus replication holoenzyme f8-a22-e4 complex with dna (191 MDa, Complex from Monkeypox virus)
- Dna polymerase (120 kDa, Protein from Monkeypox virus)
- E4r (27 kDa, Protein from Monkeypox virus)
- Dna polymerase processivity factor component a20 (49 kDa, Protein from Monkeypox virus)
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 8hoy
Q-score: 0.292
Xu Y, Wu Y, Zhang Y, Fan R, Yang Y, Li D, Zhu S, Yang B, Zhang Z, Dong C
Cell Res (2023) 33 pp. 479-482 [ DOI: doi:10.1038/s41422-023-00796-1 Pubmed: 36973539 ]
- Dna polymerase processivity factor component a20 (49 kDa, Protein from Monkeypox virus)
- Dna polymerase (120 kDa, Protein from Monkeypox virus)
- E4r (27 kDa, Protein from Monkeypox virus)
- Monkeypox virus replication holoenzyme f8-a22-e4 complex without dna (191 MDa, Complex from Monkeypox virus)
Monkeypox virus DNA replication holoenzyme F8, A22 and E4 complex in an apo form
Resolution: 3.05 Å
EM Method: Single-particle
Fitted PDBs: 8hdz
Q-score: 0.273
Xu Y, Wu Y, Zhang Y, Fan R, Yang Y, Li D, Zhu S, Yang B, Zhang Z, Dong C
Cell Res (2023) 33 pp. 479-482 [ Pubmed: 36973539 DOI: doi:10.1038/s41422-023-00796-1 ]
- E4 uracil-dna glycosylase (27 kDa, Protein from Monkeypox virus)
- A22 dna replication processivity factor (49 kDa, Protein from Monkeypox virus)
- F8 dna polymerase (120 kDa, Protein from Monkeypox virus)
- Monkeypox virus replication holoenzyme f8-a22-e4 complex (191 MDa, Complex from Monkeypox virus)