MPOX E5 hexamer 2ATP, 2ADP, and ssDNA binding comformation
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8wh2
Q-score: 0.516
Xu Y, Wu Y, Zhang Y, Gao K, Wu X, Yang Y, Li D, Yang B, Zhang Z, Dong C
Sci Adv (2024) 10 pp. eadl1150-eadl1150 [ DOI: doi:10.1126/sciadv.adl1150 Pubmed: 39167653 ]
- Dna (5'-d(p*cp*cp*cp*cp*c)-3') (1 kDa, DNA from Spodoptera frugiperda)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Mpox helicase (Complex from Monkeypox virus)
- Uncoating factor opg117 (90 kDa, Protein from Monkeypox virus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
MPOX E5 hexamer AMP-PNP and ssDNA bound form with clear primase domain
Resolution: 2.91 Å
EM Method: Single-particle
Fitted PDBs: 8wgy
Q-score: 0.494
Xu Y, Wu Y, Zhang Y, Gao K, Wu X, Yang Y, Li D, Yang B, Zhang Z, Dong C
Sci Adv (2024) 10 pp. eadl1150-eadl1150 [ DOI: doi:10.1126/sciadv.adl1150 Pubmed: 39167653 ]
- Mpox helicase (Complex from Monkeypox virus)
- Uncoating factor opg117 (90 kDa, Protein from Monkeypox virus)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna (5'-d(p*cp*cp*c)-3') (822 Da, DNA from Spodoptera frugiperda)
- Zinc ion (65 Da, Ligand)
MPOX E5 double hexamer ssDNA bound conformation
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 8wgz
Q-score: 0.273
Xu Y, Wu Y, Zhang Y, Gao K, Wu X, Yang Y, Li D, Yang B, Zhang Z, Dong C
Sci Adv (2024) 10 pp. eadl1150-eadl1150 [ DOI: doi:10.1126/sciadv.adl1150 Pubmed: 39167653 ]
- Dna (5'-d(p*cp*cp*cp*cp*c)-3') (1 kDa, DNA from Spodoptera frugiperda)
- Mpox helicase (Complex from Monkeypox virus)
- Uncoating factor opg117 (90 kDa, Protein from Monkeypox virus)
MPOX E5 hexamer ssDNA and AMP-PNP bound conformation
Resolution: 2.51 Å
EM Method: Single-particle
Fitted PDBs: 8wh0
Q-score: 0.589
Xu Y, Wu Y, Zhang Y, Gao K, Wu X, Yang Y, Li D, Yang B, Zhang Z, Dong C
Sci Adv (2024) 10 pp. eadl1150-eadl1150 [ DOI: doi:10.1126/sciadv.adl1150 Pubmed: 39167653 ]
- Dna (5'-d(p*cp*cp*cp*cp*c)-3') (1 kDa, DNA from Spodoptera frugiperda)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Mpox helicase (Complex from Monkeypox virus)
- Uncoating factor opg117 (90 kDa, Protein from Monkeypox virus)
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 8wh3
Q-score: 0.425
Xu Y, Wu Y, Zhang Y, Gao K, Wu X, Yang Y, Li D, Yang B, Zhang Z, Dong C
Sci Adv (2024) 10 pp. eadl1150-eadl1150 [ DOI: doi:10.1126/sciadv.adl1150 Pubmed: 39167653 ]
- Mpox helicase (Complex from Monkeypox virus)
- Uncoating factor opg117 (90 kDa, Protein from Monkeypox virus)
MPOX E5 hexamer ssDNA bound apo conformation
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 8wh4
Q-score: 0.404
Xu Y, Wu Y, Zhang Y, Gao K, Wu X, Yang Y, Li D, Yang B, Zhang Z, Dong C
Sci Adv (2024) 10 pp. eadl1150-eadl1150 [ DOI: doi:10.1126/sciadv.adl1150 Pubmed: 39167653 ]
- Dna (5'-d(p*cp*cp*cp*c)-3') (1 kDa, DNA from Spodoptera frugiperda)
- Mpox helicase (Complex from Monkeypox virus)
- Uncoating factor opg117 (90 kDa, Protein from Monkeypox virus)
MPOX E5 hexamer ADP and ssDNA bound and clear primase domain conformation
Resolution: 3.27 Å
EM Method: Single-particle
Fitted PDBs: 8wh6
Q-score: 0.462
Xu Y, Wu Y, Zhang Y, Gao K, Wu X, Yang Y, Li D, Yang B, Zhang Z, Dong C
Sci Adv (2024) 10 pp. eadl1150-eadl1150 [ DOI: doi:10.1126/sciadv.adl1150 Pubmed: 39167653 ]
- Mpox helicase (Complex from Monkeypox virus)
- Uncoating factor opg117 (90 kDa, Protein from Monkeypox virus)
- Dna (5'-d(p*cp*cp*c)-3') (822 Da, DNA from Spodoptera frugiperda)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Zinc ion (65 Da, Ligand)
Photorhabdus luminescens TcdA1 prepore-to-pore intermediate, K1179W mutant
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8cpz
Q-score: 0.526
Ng'ang'a PN, Folz J, Kucher S, Roderer D, Xu Y, Sitsel O, Belyy A, Prumbaum D, Kuhnemuth R, Assafa TE, Dong M, Seidel CAM, Bordignon E, Raunser S
Sci Adv (2025) 11 pp. eadr2019-eadr2019 [ DOI: doi:10.1126/sciadv.adr2019 Pubmed: 39752508 ]
- Tcda1 (1 MDa, Complex from Photorhabdus luminescens)
- Tcda1 (285 kDa, Protein from Photorhabdus luminescens)
Photorhabdus luminescens TcdA1 prepore-to-pore intermediate, K567W K2008W mutant
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8cq0
Q-score: 0.466
Ng'ang'a PN, Folz J, Kucher S, Roderer D, Xu Y, Sitsel O, Belyy A, Prumbaum D, Kuhnemuth R, Assafa TE, Dong M, Seidel CAM, Bordignon E, Raunser S
Sci Adv (2025) 11 pp. eadr2019-eadr2019 [ DOI: doi:10.1126/sciadv.adr2019 Pubmed: 39752508 ]
- Tcda1 (1 MDa, Complex from Photorhabdus luminescens)
- Tcda1 (285 kDa, Protein from Photorhabdus luminescens)
Photorhabdus luminescens TcdA1 prepore-to-pore intermediate, C16S, C20S, C870S, T1279C mutant
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8cq2
Q-score: 0.448
Ng'ang'a PN, Folz J, Kucher S, Roderer D, Xu Y, Sitsel O, Belyy A, Prumbaum D, Kuhnemuth R, Assafa TE, Dong M, Seidel CAM, Bordignon E, Raunser S
Sci Adv (2025) 11 pp. eadr2019-eadr2019 [ Pubmed: 39752508 DOI: doi:10.1126/sciadv.adr2019 ]
- Tcda1 (285 kDa, Protein from Photorhabdus luminescens)
- Tcda1 (1 MDa, Complex from Photorhabdus luminescens)