Cryo-EM structure of Pseudomonas aeruginosa SutA transcription activation complex
Resolution: 5.8 Å
EM Method: Single-particle
Fitted PDBs: 7f0r
Q-score: 0.16
He D, You L, Wu X, Shi J, Wen A, Yan Z, Mu W, Fang C, Feng Y, Zhang Y
Nat Commun (2022) 13 pp. 4204-4204 [ DOI: doi:10.1038/s41467-022-31871-7 Pubmed: 35859063 ]
- Transcriptional factor suta (11 kDa, Protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))
- Dna (Complex)
- Pseudomonas aeruginosa suta transcription activation complex (433 kDa, Complex from Pseudomonas aeruginosa PAO1)
- Dna (70-mer) (21 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit omega (9 kDa, Protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))
- Dna-directed rna polymerase subunit alpha (38 kDa, Protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (151 kDa, Protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))
- Dna (70-mer) (21 kDa, DNA from synthetic construct)
- Pseudomonas aeruginosa suta transcription activation (Complex)
- Rna polymerase sigma factor rpos (39 kDa, Protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))
Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes
Resolution: 4.04 Å
EM Method: Single-particle
Fitted PDBs: 7vf9
Q-score: 0.373
He D, You L, Wu X, Shi J, Wen A, Yan Z, Mu W, Fang C, Feng Y, Zhang Y
Nat Commun (2022) 13 pp. 4204-4204 [ DOI: doi:10.1038/s41467-022-31871-7 Pubmed: 35859063 ]
- Dna-directed rna polymerase subunit alpha (38 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Pseudomonas aeruginosa rnap sigmas holoenzyme complexes (432 kDa, Complex from Pseudomonas aeruginosa PAO1)
- Dna-directed rna polymerase subunit omega (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Zinc ion (65 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta (151 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Rna polymerase sigma factor rpos (38 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Pseudomonas aeruginosa PAO1)
Resolution: 3.13 Å
EM Method: Single-particle
Fitted PDBs: 7xl3
Q-score: 0.468
He D, You L, Wu X, Shi J, Wen A, Yan Z, Mu W, Fang C, Feng Y, Zhang Y
Nat Commun (2022) 13 pp. 4204-4204 [ DOI: doi:10.1038/s41467-022-31871-7 Pubmed: 35859063 ]
- Dna-directed rna polymerase subunit alpha (38 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Dna-directed rna polymerase subunit omega (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Transcriptional factor suta (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Zinc ion (65 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta (151 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Pseudomonas aeruginosa rnap sigmas holoenzyme with transcription factor suta complex(open lobe) (433 kDa, Complex from Pseudomonas aeruginosa PAO1)
- Rna polymerase sigma factor rpos (38 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Pseudomonas aeruginosa PAO1)
Resolution: 3.86 Å
EM Method: Single-particle
Fitted PDBs: 7xl4
Q-score: 0.39
He D, You L, Wu X, Shi J, Wen A, Yan Z, Mu W, Fang C, Feng Y, Zhang Y
Nat Commun (2022) 13 pp. 4204-4204 [ DOI: doi:10.1038/s41467-022-31871-7 Pubmed: 35859063 ]
- Dna-directed rna polymerase subunit alpha (38 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Dna-directed rna polymerase subunit omega (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Pseudomonas aeruginosa rnap sigmas holoenzyme with transcription factor suta complex(closed lobe) (433 kDa, Complex from Pseudomonas aeruginosa PAO1)
- Transcriptional factor suta (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Zinc ion (65 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta (151 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Rna polymerase sigma factor rpos (38 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Pseudomonas aeruginosa PAO1)