CryoEM structure of Gq coupled MRGPRX4 with agonist DCA-3P
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8k4s
Q-score: 0.521
Yang J, Zhao T, Fan J, Zou H, Lan G, Guo F, Shi Y, Ke H, Yu H, Yue Z, Wang X, Bai Y, Li S, Liu Y, Wang X, Chen Y, Li Y, Lei X
Cell (2024) 187 pp. 7164-7182.e18 [ DOI: doi:10.1016/j.cell.2024.10.001 Pubmed: 39476841 ]
- (4~{r})-4-[(3~{r},5~{r},8~{r},9~{s},10~{s},12~{s},13~{r},14~{s},17~{r})-10,13-dimethyl-12-oxidanyl-3-phosphonooxy-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1~{h}-cyclopenta[a]phenanthren-17-yl]pentanoic acid (472 Da, Ligand)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,mas-related g-protein coupled receptor member x4,green fluorescent protein (79 kDa, Protein from Aequorea victoria)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (8 kDa, Protein from Homo sapiens)
- Complex of mrgprx4 with gq and agonist dca-3p (Complex from Homo sapiens)
CryoEM structure of Gq coupled MRGPRX4 with agonist DCA-3P, local
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8kex
Q-score: 0.416
Yang J, Zhao T, Fan J, Zou H, Lan G, Guo F, Shi Y, Ke H, Yu H, Yue Z, Wang X, Bai Y, Li S, Liu Y, Wang X, Chen Y, Li Y, Lei X
Cell (2024) 187 pp. 7164-7182.e18 [ DOI: doi:10.1016/j.cell.2024.10.001 Pubmed: 39476841 ]
- Cryo-em structure of mrgprx4 complex with agonist dca-3p (Complex from Homo sapiens)
- Soluble cytochrome b562,mas-related g-protein coupled receptor member x4,green fluorescent protein (79 kDa, Protein from Aequorea victoria)
- (4~{r})-4-[(3~{r},5~{r},8~{r},9~{s},10~{s},12~{s},13~{r},14~{s},17~{r})-10,13-dimethyl-12-oxidanyl-3-phosphonooxy-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1~{h}-cyclopenta[a]phenanthren-17-yl]pentanoic acid (472 Da, Ligand)
The Cryo-EM structure of MPXV E5 apo conformation
Resolution: 3.32 Å
EM Method: Single-particle
Fitted PDBs: 8xj6
Q-score: 0.362
Zhang W, Liu Y, Yang M, Yang J, Shao Z, Gao Y, Jiang X, Cui R, Zhang Y, Zhao X, Shao Q, Cao C, Li H, Li L, Liu H, Gao H, Gan J
Cell Discov (2024) 10 pp. 67-67 [ DOI: doi:10.1038/s41421-024-00680-1 Pubmed: 38914567 ]
- Monkeypox virus (Virus from Monkeypox virus)
- Amp phosphoramidate (426 Da, Ligand)
- Monkeypox virus e5 (90 kDa, Protein from Monkeypox virus)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Zinc ion (65 Da, Ligand)
The Cryo-EM structure of MPXV E5 in complex with DNA
Resolution: 2.74 Å
EM Method: Single-particle
Fitted PDBs: 8xj7
Q-score: 0.541
Zhang W, Liu Y, Yang M, Yang J, Shao Z, Gao Y, Jiang X, Cui R, Zhang Y, Zhao X, Shao Q, Cao C, Li H, Li L, Liu H, Gao H, Gan J
Cell Discov (2024) 10 pp. 67-67 [ DOI: doi:10.1038/s41421-024-00680-1 Pubmed: 38914567 ]
- Monkeypox virus (Virus from Monkeypox virus)
- Magnesium ion (24 Da, Ligand)
- Monkeypox virus e5 (90 kDa, Protein from Monkeypox virus)
- Dna (70-mer) (21 kDa, DNA from Monkeypox virus)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
The Cryo-EM structure of MPXV E5 C-terminal in complex with DNA
Resolution: 2.67 Å
EM Method: Single-particle
Fitted PDBs: 8xj8
Q-score: 0.189
Zhang W, Liu Y, Yang M, Yang J, Shao Z, Gao Y, Jiang X, Cui R, Zhang Y, Zhao X, Shao Q, Cao C, Li H, Li L, Liu H, Gao H, Gan J
Cell Discov (2024) 10 pp. 67-67 [ DOI: doi:10.1038/s41421-024-00680-1 Pubmed: 38914567 ]
- Monkeypox virus (Virus from Monkeypox virus)
- Monkeypox virus e5 (53 kDa, Protein from Monkeypox virus)
- Magnesium ion (24 Da, Ligand)
- Dna (70-mer) (21 kDa, DNA from Monkeypox virus)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)