Cryo-EM structure of CBF1-CCAN bound topologically to centromeric DNA
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8ovw
Q-score: 0.386
Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D
Sci Adv (2023) 9 pp. eadg7480-eadg7480 [ Pubmed: 37506202 DOI: doi:10.1126/sciadv.adg7480 ]
- C0n3 dna (47 kDa, DNA from Saccharomyces cerevisiae)
- Inner kinetochore subunit iml3 (28 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm16 (21 kDa, Protein from Saccharomyces cerevisiae)
- A complex of cbf1-ccan bound to centromeric c0n3 dna (Complex from Saccharomyces cerevisiae)
- C0n3 dna (47 kDa, DNA from Saccharomyces cerevisiae)
- Inner kinetochore subunit ame1 (37 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit wip1 (10 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm21 (43 kDa, Protein from Saccharomyces cerevisiae)
- Centromere-binding protein 1 (39 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit chl4 (52 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf19 (42 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf3 (84 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp1 (27 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp2 (17 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit okp1 (47 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit cnn1 (41 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm22 (27 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of yeast CENP-OPQU+ bound to the CENP-A N-terminus
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8ovx
Q-score: 0.283
Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D
Sci Adv (2023) 9 pp. eadg7480-eadg7480 [ Pubmed: 37506202 DOI: doi:10.1126/sciadv.adg7480 ]
- A complex of cbf1-ccan bound to centromeric c0n3 dna (Complex from Saccharomyces cerevisiae)
- Inner kinetochore subunit ame1 (37 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm21 (43 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp1 (27 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf19 (42 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp2 (17 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit okp1 (47 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of CBF1-CCAN bound topologically to a centromeric CENP-A nucleosome
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8ow0
Q-score: 0.2
Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D
Sci Adv (2023) 9 pp. eadg7480-eadg7480 [ Pubmed: 37506202 DOI: doi:10.1126/sciadv.adg7480 ]
- Histone h2a.1 (14 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit wip1 (10 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm21 (43 kDa, Protein from Saccharomyces cerevisiae)
- Centromere-binding protein 1 (39 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp2 (17 kDa, Protein from Saccharomyces cerevisiae)
- A complex of cbf1-ccan bound to centromeric c0n3 dna (Complex from Saccharomyces cerevisiae)
- C0n3 dna (47 kDa, DNA from Saccharomyces cerevisiae)
- Inner kinetochore subunit ame1 (37 kDa, Protein from Saccharomyces cerevisiae)
- Histone h2b.1 (14 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit chl4 (52 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit okp1 (47 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit cnn1 (41 kDa, Protein from Saccharomyces cerevisiae)
- C0n3 dna (47 kDa, DNA from Saccharomyces cerevisiae)
- Inner kinetochore subunit iml3 (28 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm16 (21 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf19 (42 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm22 (27 kDa, Protein from Saccharomyces cerevisiae)
- Histone h4 (11 kDa, Protein from Saccharomyces cerevisiae)
- Histone h3-like centromeric protein cse4 (26 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp1 (27 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf3 (84 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of the yeast Inner kinetochore bound to a CENP-A nucleosome.
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8ow1
Q-score: 0.165
Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D
Sci Adv (2023) 9 pp. eadg7480-eadg7480 [ Pubmed: 37506202 DOI: doi:10.1126/sciadv.adg7480 ]
- Histone h2a.1 (14 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit wip1 (10 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm21 (43 kDa, Protein from Saccharomyces cerevisiae)
- Centromere-binding protein 1 (39 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp2 (17 kDa, Protein from Saccharomyces cerevisiae)
- A complex of cbf1-ccan bound to centromeric c0n3 dna (Complex from Saccharomyces cerevisiae)
- Inner kinetochore subunit ame1 (37 kDa, Protein from Saccharomyces cerevisiae)
- Histone h2b.1 (14 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit chl4 (52 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit okp1 (47 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit cnn1 (41 kDa, Protein from Saccharomyces cerevisiae)
- C0n3 (47 kDa, DNA from Saccharomyces cerevisiae)
- Inner kinetochore subunit iml3 (28 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm16 (21 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf19 (42 kDa, Protein from Saccharomyces cerevisiae)
- C0n3 (47 kDa, DNA from Saccharomyces cerevisiae)
- Centromere dna-binding protein complex cbf3 subunit c (56 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm22 (27 kDa, Protein from Saccharomyces cerevisiae)
- Histone h4 (11 kDa, Protein from Saccharomyces cerevisiae)
- Histone h3-like centromeric protein cse4 (26 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp1 (27 kDa, Protein from Saccharomyces cerevisiae)
- Centromere dna-binding protein complex cbf3 subunit b (71 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf3 (84 kDa, Protein from Saccharomyces cerevisiae)
- Suppressor of kinetochore protein 1 (22 kDa, Protein from Saccharomyces cerevisiae)
Multibody map of the CENP-A nucleosome as part of the inner kinetochore
Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D
Sci Adv (2023) 9 pp. eadg7480-eadg7480 [ Pubmed: 37506202 DOI: doi:10.1126/sciadv.adg7480 ]
Multibody map of CCAN(Topo) bound to CON3 DNA as part of the Inner kinetochore.
Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D
Sci Adv (2023) 9 pp. eadg7480-eadg7480 [ Pubmed: 37506202 DOI: doi:10.1126/sciadv.adg7480 ]
Multibody map of CCAN(Non-Topo) bound to C0N3 DNA as part of the inner kinetochore.
Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D
Sci Adv (2023) 9 pp. eadg7480-eadg7480 [ Pubmed: 37506202 DOI: doi:10.1126/sciadv.adg7480 ]
Multibody map of CBF3:CENP-HIK as part of the inner kinetochore
Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D
Sci Adv (2023) 9 pp. eadg7480-eadg7480 [ Pubmed: 37506202 DOI: doi:10.1126/sciadv.adg7480 ]
Consensus map of the yeast inner kinetochore
Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D
Sci Adv (2023) 9 pp. eadg7480-eadg7480 [ Pubmed: 37506202 DOI: doi:10.1126/sciadv.adg7480 ]