CryoEM structure of Gq coupled MRGPRX4 with agonist DCA-3P
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8k4s
Q-score: 0.521
Yang J, Zhao T, Fan J, Zou H, Lan G, Guo F, Shi Y, Ke H, Yu H, Yue Z, Wang X, Bai Y, Li S, Liu Y, Wang X, Chen Y, Li Y, Lei X
Cell (2024) 187 pp. 7164-7182.e18 [ DOI: doi:10.1016/j.cell.2024.10.001 Pubmed: 39476841 ]
- (4~{r})-4-[(3~{r},5~{r},8~{r},9~{s},10~{s},12~{s},13~{r},14~{s},17~{r})-10,13-dimethyl-12-oxidanyl-3-phosphonooxy-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1~{h}-cyclopenta[a]phenanthren-17-yl]pentanoic acid (472 Da, Ligand)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,mas-related g-protein coupled receptor member x4,green fluorescent protein (79 kDa, Protein from Aequorea victoria)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (8 kDa, Protein from Homo sapiens)
- Complex of mrgprx4 with gq and agonist dca-3p (Complex from Homo sapiens)
CryoEM structure of Gq coupled MRGPRX4 with agonist DCA-3P, local
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8kex
Q-score: 0.416
Yang J, Zhao T, Fan J, Zou H, Lan G, Guo F, Shi Y, Ke H, Yu H, Yue Z, Wang X, Bai Y, Li S, Liu Y, Wang X, Chen Y, Li Y, Lei X
Cell (2024) 187 pp. 7164-7182.e18 [ DOI: doi:10.1016/j.cell.2024.10.001 Pubmed: 39476841 ]
- Cryo-em structure of mrgprx4 complex with agonist dca-3p (Complex from Homo sapiens)
- Soluble cytochrome b562,mas-related g-protein coupled receptor member x4,green fluorescent protein (79 kDa, Protein from Aequorea victoria)
- (4~{r})-4-[(3~{r},5~{r},8~{r},9~{s},10~{s},12~{s},13~{r},14~{s},17~{r})-10,13-dimethyl-12-oxidanyl-3-phosphonooxy-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1~{h}-cyclopenta[a]phenanthren-17-yl]pentanoic acid (472 Da, Ligand)
The cryo-EM structure of human sphingomyelin synthase-related protein in complex with ceramide
Resolution: 3.45 Å
EM Method: Single-particle
Fitted PDBs: 8ijq
Q-score: 0.461
Resolution: 3.29 Å
EM Method: Single-particle
Fitted PDBs: 8ijr
Q-score: 0.485
Hu K, Zhang Q, Chen Y, Yang J, Xia Y, Rao B, Li S, Shen Y, Cao M, Lu H, Qin A, Jiang XC, Yao D, Zhao J, Zhou L, Cao Y
Nat Struct Mol Biol (2024) 31 pp. 884-895 [ Pubmed: 38388831 DOI: doi:10.1038/s41594-024-01237-2 ]
- (2s)-1-(hexadecanoyloxy)-3-hydroxypropan-2-yl (11z)-octadec-11-enoate (594 Da, Ligand)
- The cryo-em structure of human sphingomyelin synthase-related protein in complex with diacylglycerol/phosphoethanolamine (Complex from Homo sapiens)
- Sphingomyelin synthase-related protein 1 (48 kDa, Protein from Homo sapiens)
- Phosphoric acid mono-(2-amino-ethyl) ester (141 Da, Ligand)
Resolution: 3.74 Å
EM Method: Single-particle
Fitted PDBs: 8w9w
Q-score: 0.377
Hu K, Zhang Q, Chen Y, Yang J, Xia Y, Rao B, Li S, Shen Y, Cao M, Lu H, Qin A, Jiang XC, Yao D, Zhao J, Zhou L, Cao Y
Nat Struct Mol Biol (2024) 31 pp. 884-895 [ Pubmed: 38388831 DOI: doi:10.1038/s41594-024-01237-2 ]
- Sphingomyelin synthase-related protein 1 (31 kDa, Protein from Homo sapiens)
- Phosphoric acid mono-(2-amino-ethyl) ester (141 Da, Ligand)
- ~{n}-[(~{z},2~{s},3~{r})-1,3-bis(oxidanyl)heptadec-4-en-2-yl]dodecanamide (467 Da, Ligand)
- The cryo-em structure of human sphingomyelin synthase-related protein in complex with ceramide/phosphoethanolamine (Complex from Homo sapiens)
The cryo-EM structure of human sphingomyelin synthase-related protein
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8w9y
Q-score: 0.392