SUMOylated apoAPC/C with repositioned APC2 WHB domain
Resolution: 3.78 Å
EM Method: Single-particle
Fitted PDBs: 6tnt
Q-score: 0.39
Yatskevich S, Kroonen JS, Alfieri C, Tischer T, Howes AC, Clijsters L, Yang J, Zhang Z, Yan K, Vertegaal ACO, Barford D
Cell Rep (2021) 34 pp. 108929-108929 [ Pubmed: 33789095 DOI: doi:10.1016/j.celrep.2021.108929 ]
- Body 2 with repositioned apc2 whb domain after multi body refinement (Complex from Homo sapiens)
- Anaphase-promoting complex subunit 7 (66 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Anaphase-promoting complex subunit 16 (11 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 4 (92 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 2 (93 kDa, Protein from Homo sapiens)
- Unidentified peptide (1 kDa, Protein from unidentified)
- Anaphase-promoting complex subunit 10 (21 kDa, Protein from Homo sapiens)
- Cell division cycle protein 23 homolog (68 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 11 (9 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 5 (85 kDa, Protein from Homo sapiens)
- Sumoylated apoapc/c with repositioned apc2 whb domain (Complex)
- Unidentified peptide (Complex from unidentified)
- Anaphase-promoting complex subunit 1 (216 kDa, Protein from Homo sapiens)
- Cell division cycle protein 27 homolog (91 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 15 (14 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 13 (8 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit cdc26 (9 kDa, Protein from Homo sapiens)
- Cell division cycle protein 16 homolog (71 kDa, Protein from Homo sapiens)
Structure of inner kinetochore CCAN-Cenp-A complex
Resolution: 4.15 Å
EM Method: Single-particle
Fitted PDBs: 6qld
Q-score: 0.328
Yan K, Yang J, Zhang Z, McLaughlin SH, Chang L, Fasci D, Ehrenhofer-Murray AE, Heck AJR, Barford D
Nature (2019) 574 pp. 278-282 [ Pubmed: 31578520 DOI: doi:10.1038/s41586-019-1609-1 ]
- Kinetochore (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) cerevisiae)
- Inner kinetochore subunit okp1 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histone h4 (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Inner kinetochore subunit mif2 (2 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Inner kinetochore subunit nkp2 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Inner kinetochore subunit mcm21 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histone h2a.1 (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histone h2b.2 (10 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Inner kinetochore ccan complex assembled onto a centromeric nucleosome (Complex)
- Histones (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (125-mer) (38 kDa, DNA from Escherichia coli)
- Inner kinetochore subunit ctf19 (31 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Inner kinetochore subunit iml3 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Inner kinetochore subunit chl4 (51 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histone h2b.1 (10 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Inner kinetochore subunit mcm16 (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (Complex from Escherichia coli)
- Histone h2a.1 (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Inner kinetochore subunit mcm22 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histone h3-like centromeric protein cse4 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (125-mer) (38 kDa, DNA from Escherichia coli)
- Inner kinetochore subunit ctf3 (47 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Inner kinetochore subunit nkp1 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histone h3-like centromeric protein cse4 (10 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Inner kinetochore subunit ame1 (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Structure of inner kinetochore CCAN complex
Resolution: 3.55 Å
EM Method: Single-particle
Fitted PDBs: 6qle
Q-score: 0.415
Yan K, Yang J, Zhang Z, McLaughlin SH, Chang L, Fasci D, Ehrenhofer-Murray AE, Heck AJR, Barford D
Nature (2019) 574 pp. 278-282 [ DOI: doi:10.1038/s41586-019-1609-1 Pubmed: 31578520 ]
- Inner kinetochore subunit mcm21 (43 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm22,inner kinetochore subunit mcm22,inner kinetochore subunit mcm22,inner kinetochore subunit mcm22 (21 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit chl4 (52 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf19 (42 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore ccan complex (Complex from Saccharomyces cerevisiae)
- Central kinetochore subunit ctf3,inner kinetochore subunit ctf3,central kinetochore subunit ctf3,inner kinetochore subunit ctf3 (46 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp1 (27 kDa, Protein from Saccharomyces cerevisiae)
- Central kinetochore subunit mcm16,central kinetochore subunit mcm16,inner kinetochore subunit mcm16,mcm16p (20 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ame1,inner kinetochore subunit ame1,inner kinetochore subunit ame1,inner kinetochore subunit ame1 (20 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit okp1,inner kinetochore subunit okp1,inner kinetochore subunit okp1,inner kinetochore subunit okp1 (30 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit iml3 (28 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp2 (17 kDa, Protein from Saccharomyces cerevisiae)
Structure of inner kinetochore CCAN complex with mask1
Resolution: 3.45 Å
EM Method: Single-particle
Fitted PDBs: 6qlf
Q-score: 0.484
Yan K, Yang J, Zhang Z, McLaughlin SH, Chang L, Fasci D, Ehrenhofer-Murray AE, Heck AJR, Barford D
Nature (2019) 574 pp. 278-282 [ Pubmed: 31578520 DOI: doi:10.1038/s41586-019-1609-1 ]
- Inner kinetochore ccan complex (Complex from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp1 (27 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit chl4 (53 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit okp1 (47 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ame1 (37 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf19 (42 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp2 (17 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit iml3 (28 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm21 (43 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of the CBF3-core-Ndc10-DBD complex of the budding yeast kinetochore
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6gyp
Q-score: 0.402
Yan K, Zhang Z, Yang J, McLaughlin SH, Barford D
Nat Struct Mol Biol (2018) 25 pp. 1103-1110 [ DOI: doi:10.1038/s41594-018-0154-1 Pubmed: 30478265 ]
- Centromere dna-binding protein complex cbf3 subunit c (56 kDa, Protein from Saccharomyces cerevisiae S288C)
- Centromere dna-binding protein complex cbf3 subunit a (112 kDa, Protein from Saccharomyces cerevisiae S288C)
- Methionine (149 Da, Ligand)
- Asparagine (132 Da, Ligand)
- Glutamine (146 Da, Ligand)
- Suppressor of kinetochore protein 1 (22 kDa, Protein from Saccharomyces cerevisiae S288C)
- Centromere dna-binding protein complex cbf3 subunit b (71 kDa, Protein from Saccharomyces cerevisiae S288C)
- Centromere dna-binding protein complex cbf3 subunit b (66 kDa, Protein from Saccharomyces cerevisiae S288C)
- Threonine (119 Da, Ligand)
- Phenylalanine (165 Da, Ligand)
- Cbf3-core-ndc10-dbd complex (Complex from Saccharomyces cerevisiae S288C)
- Arginine (175 Da, Ligand)
Cryo-EM structure of the CBF3-CEN3 complex of the budding yeast kinetochore
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 6gys
Q-score: 0.245
Yan K, Zhang Z, Yang J, McLaughlin SH, Barford D
Nat Struct Mol Biol (2018) 25 pp. 1103-1110 [ DOI: doi:10.1038/s41594-018-0154-1 Pubmed: 30478265 ]
- Centromere dna-binding protein complex cbf3 subunit c (56 kDa, Protein from Saccharomyces cerevisiae)
- Suppressor of kinetochore protein 1 (22 kDa, Protein from Saccharomyces cerevisiae)
- Dna (52-mer) (16 kDa, DNA from Saccharomyces cerevisiae)
- Centromere dna-binding protein complex cbf3 subunit a (112 kDa, Protein from Saccharomyces cerevisiae)
- Dna (52-mer) (15 kDa, DNA from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand from Saccharomyces cerevisiae)
- Centromere dna-binding protein complex cbf3 subunit b (71 kDa, Protein from Saccharomyces cerevisiae)
- Cryo-em structure of the cbf3-cen3 complex of the budding yeast kinetochore (Complex from Saccharomyces cerevisiae)
Cryo-EM structure of the CBF3-msk complex of the budding yeast kinetochore
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6gyu
Q-score: 0.47
Yan K, Zhang Z, Yang J, McLaughlin SH, Barford D
Nat Struct Mol Biol (2018) 25 pp. 1103-1110 [ DOI: doi:10.1038/s41594-018-0154-1 Pubmed: 30478265 ]
- Centromere dna-binding protein complex cbf3 subunit b (71 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Centromere dna-binding protein complex cbf3 subunit c (56 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Cbf3-msk complex (Complex from Saccharomyces cerevisiae S288C)
- Centromere dna-binding protein complex cbf3 subunit a (112 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Suppressor of kinetochore protein 1 (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Zinc ion (65 Da, Ligand)
- Centromere dna-binding protein complex cbf3 subunit b (66 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))