Outer kinetochore Ndc80-Dam1 alpha/beta-tubulin complex
Resolution: 3.54 Å
EM Method: Single-particle
Fitted PDBs: 8qau
Q-score: 0.343
Muir KW, Batters C, Dendooven T, Yang J, Zhang Z, Burt A, Barford D
bioRxiv (2023) [ DOI: doi:10.1101/2023.07.20.549907 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Dash complex subunit dam1 (38 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Tubulin alpha-1a chain (50 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Kinetochore protein ndc80 (80 kDa, Protein from Saccharomyces cerevisiae)
- Taxol (853 Da, Ligand)
- Outer kinetochore ndc80-nuf2 ch domains bound to dam1 in complex with alpha-beta tubulin (Complex from Saccharomyces cerevisiae)
- Kinetochore protein nuf2 (53 kDa, Protein from Saccharomyces cerevisiae)
Outer kinetochore Dam1 protomer dimer Ndc80-Nuf2 coiled-coil complex
Resolution: 3.15 Å
EM Method: Single-particle
Fitted PDBs: 8q84
Q-score: 0.294
Muir KW, Batters C, Dendooven T, Yang J, Zhang Z, Burt A, Barford D
Science (2023) 382 pp. 1184-1190 [ Pubmed: 38060647 DOI: doi:10.1126/science.adj8736 ]
- Dash complex subunit dad3 (10 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit dam1 (38 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit spc19 (18 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit dad1 (10 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit ask1 (32 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit hsk3 (8 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit spc34 (34 kDa, Protein from Saccharomyces cerevisiae)
- Kinetochore protein ndc80 (80 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit duo1 (27 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit dad2 (15 kDa, Protein from Saccharomyces cerevisiae)
- Outer kinetochore dam1 protomer dimer with staple and ndc80-nuf2 coiled-coils (673 GDa, Complex from Saccharomyces cerevisiae)
- Dash complex subunit dad4 (8 kDa, Protein from Saccharomyces cerevisiae)
- Kinetochore protein nuf2 (53 kDa, Protein from Saccharomyces cerevisiae)
Outer kinetochore Dam1 protomer monomer Ndc80-Nuf2 coiled-coil complex
Resolution: 3.97 Å
EM Method: Single-particle
Fitted PDBs: 8q85
Q-score: 0.277
Muir KW, Batters C, Dendooven T, Yang J, Zhang Z, Burt A, Barford D
Science (2023) 382 pp. 1184-1190 [ Pubmed: 38060647 DOI: doi:10.1126/science.adj8736 ]
- Outer kinetochore dam1 protomer monomer with staple and ndc80-nuf2 coiled-coils (673 GDa, Complex from Saccharomyces cerevisiae)
- Dash complex subunit dad3 (10 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit dam1 (38 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit spc19 (18 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit dad1 (10 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit ask1 (32 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit hsk3 (8 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit spc34 (34 kDa, Protein from Saccharomyces cerevisiae)
- Kinetochore protein ndc80 (80 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit duo1 (27 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit dad2 (15 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit dad4 (8 kDa, Protein from Saccharomyces cerevisiae)
- Kinetochore protein nuf2 (53 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of CBF1-CCAN bound topologically to centromeric DNA
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8ovw
Q-score: 0.386
Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D
Sci Adv (2023) 9 pp. eadg7480-eadg7480 [ DOI: doi:10.1126/sciadv.adg7480 Pubmed: 37506202 ]
- C0n3 dna (47 kDa, DNA from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp1 (27 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit chl4 (52 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf3 (84 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit cnn1 (41 kDa, Protein from Saccharomyces cerevisiae)
- A complex of cbf1-ccan bound to centromeric c0n3 dna (Complex from Saccharomyces cerevisiae)
- Inner kinetochore subunit okp1 (47 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm21 (43 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit wip1 (10 kDa, Protein from Saccharomyces cerevisiae)
- Centromere-binding protein 1 (39 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm22 (27 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf19 (42 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ame1 (37 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp2 (17 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm16 (21 kDa, Protein from Saccharomyces cerevisiae)
- C0n3 dna (47 kDa, DNA from Saccharomyces cerevisiae)
- Inner kinetochore subunit iml3 (28 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of yeast CENP-OPQU+ bound to the CENP-A N-terminus
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8ovx
Q-score: 0.283
Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D
Sci Adv (2023) 9 pp. eadg7480-eadg7480 [ DOI: doi:10.1126/sciadv.adg7480 Pubmed: 37506202 ]
- Inner kinetochore subunit nkp1 (27 kDa, Protein from Saccharomyces cerevisiae)
- A complex of cbf1-ccan bound to centromeric c0n3 dna (Complex from Saccharomyces cerevisiae)
- Inner kinetochore subunit okp1 (47 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ame1 (37 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf19 (42 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp2 (17 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm21 (43 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of CBF1-CCAN bound topologically to a centromeric CENP-A nucleosome
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8ow0
Q-score: 0.2
Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D
Sci Adv (2023) 9 pp. eadg7480-eadg7480 [ DOI: doi:10.1126/sciadv.adg7480 Pubmed: 37506202 ]
- Inner kinetochore subunit nkp1 (27 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit chl4 (52 kDa, Protein from Saccharomyces cerevisiae)
- Histone h3-like centromeric protein cse4 (26 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit cnn1 (41 kDa, Protein from Saccharomyces cerevisiae)
- A complex of cbf1-ccan bound to centromeric c0n3 dna (Complex from Saccharomyces cerevisiae)
- Histone h2b.1 (14 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit okp1 (47 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit wip1 (10 kDa, Protein from Saccharomyces cerevisiae)
- Histone h2a.1 (14 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm21 (43 kDa, Protein from Saccharomyces cerevisiae)
- Histone h4 (11 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm22 (27 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ame1 (37 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm16 (21 kDa, Protein from Saccharomyces cerevisiae)
- C0n3 dna (47 kDa, DNA from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf3 (84 kDa, Protein from Saccharomyces cerevisiae)
- Centromere-binding protein 1 (39 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit iml3 (28 kDa, Protein from Saccharomyces cerevisiae)
- C0n3 dna (47 kDa, DNA from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf19 (42 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp2 (17 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of the yeast Inner kinetochore bound to a CENP-A nucleosome.
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8ow1
Q-score: 0.165
Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D
Sci Adv (2023) 9 pp. eadg7480-eadg7480 [ DOI: doi:10.1126/sciadv.adg7480 Pubmed: 37506202 ]
- Inner kinetochore subunit nkp1 (27 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit chl4 (52 kDa, Protein from Saccharomyces cerevisiae)
- Histone h3-like centromeric protein cse4 (26 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit cnn1 (41 kDa, Protein from Saccharomyces cerevisiae)
- A complex of cbf1-ccan bound to centromeric c0n3 dna (Complex from Saccharomyces cerevisiae)
- Histone h2b.1 (14 kDa, Protein from Saccharomyces cerevisiae)
- C0n3 (47 kDa, DNA from Saccharomyces cerevisiae)
- Inner kinetochore subunit okp1 (47 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit wip1 (10 kDa, Protein from Saccharomyces cerevisiae)
- Histone h2a.1 (14 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm21 (43 kDa, Protein from Saccharomyces cerevisiae)
- Histone h4 (11 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm22 (27 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ame1 (37 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm16 (21 kDa, Protein from Saccharomyces cerevisiae)
- Centromere dna-binding protein complex cbf3 subunit c (56 kDa, Protein from Saccharomyces cerevisiae)
- Suppressor of kinetochore protein 1 (22 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf3 (84 kDa, Protein from Saccharomyces cerevisiae)
- C0n3 (47 kDa, DNA from Saccharomyces cerevisiae)
- Centromere-binding protein 1 (39 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit iml3 (28 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf19 (42 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp2 (17 kDa, Protein from Saccharomyces cerevisiae)
- Centromere dna-binding protein complex cbf3 subunit b (71 kDa, Protein from Saccharomyces cerevisiae)
S. cerevisiae APC/C-Cdh1 complex
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8a3t
Q-score: 0.336
Barford D, Vazquez-Fernandez E, Zhang Z, Yang J
To Be Published
- Anaphase-promoting complex subunit cdc27 (85 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit cdc26 (14 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit 5 (79 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit doc1 (28 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit mnd2 (42 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit cdc23 (73 kDa, Protein from Saccharomyces cerevisiae)
- Cdh1 isoform 1 (62 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit 4 (75 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit 9 (30 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase promoting complex/cyclosome (apc/c) in complex with cdh1 and hsl1 (1 MDa, Complex from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit 11 (18 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit 1 (196 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Anaphase-promoting complex subunit swm1 (19 kDa, Protein from Saccharomyces cerevisiae)
- Hsl1 isoform 1 (169 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit 2 (100 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit cdc16 (96 kDa, Protein from Saccharomyces cerevisiae)
S. cerevisiae apo unphosphorylated APC/C
Resolution: 4.9 Å
EM Method: Single-particle
Fitted PDBs: 8a5y
Q-score: 0.192
Barford D, Fernandez-Vazquez E, Zhang Z, Yang J
To Be Published
- Anaphase promoting complex/cyclosome (apc/c) (1 MDa, Complex from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit cdc27 (85 kDa, Protein from Saccharomyces cerevisiae)
- Unphosphorylated apo apc/c complex (Complex from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit cdc26 (14 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit 5 (79 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit doc1 (28 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit mnd2 (42 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit cdc23 (73 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit 4 (75 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit 9 (30 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit 11 (18 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit 1 (196 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Anaphase-promoting complex subunit swm1 (19 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit 2 (100 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit cdc16 (96 kDa, Protein from Saccharomyces cerevisiae)
S. cerevisiae apo phosphorylated APC/C
Resolution: 5.4 Å
EM Method: Single-particle
Fitted PDBs: 8a61
Q-score: 0.223
Barford D, Fernandez-Vazquez E, Zhang Z, Yang J
To Be Published
- Anaphase-promoting complex subunit cdc27 (85 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit cdc26 (14 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit 5 (79 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit doc1 (28 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit mnd2 (42 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit cdc23 (73 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit 4 (75 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit 9 (30 kDa, Protein from Saccharomyces cerevisiae)
- Phosphorylated apo anaphase promoting complex/cyclosome (apc/c) (1 MDa, Complex from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit 11 (18 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit 1 (196 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit swm1 (19 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit 2 (100 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit cdc16 (96 kDa, Protein from Saccharomyces cerevisiae)