Cryo-EM map of the Saccharomyces cerevisiae PCNA clamp unloader Elg1-RFC complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8thb
Q-score: 0.489
Zheng F, Yao NY, Georgescu RE, Li H, O'Donnell ME
Sci Adv (2024) 10 pp. eadl1739-eadl1739 [ Pubmed: 38427736 DOI: doi:10.1126/sciadv.adl1739 ]
- Subunits rfc 2-5 of pcna clamp unloader elg1-rfc (Complex from Saccharomyces cerevisiae)
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Elg1 subunit of pcna clamp unloader elg1-rfc (Complex from Saccharomyces cerevisiae)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (37 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Pcna clamp unloader elg1-rfc (Complex)
- Magnesium ion (24 Da, Ligand)
- Elg1 isoform 1 (91 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM map of the Saccharomyces cerevisiae clamp unloader Elg1-RFC bound to a cracked PCNA
Resolution: 3.67 Å
EM Method: Single-particle
Fitted PDBs: 8thc
Q-score: 0.321
Zheng F, Yao NY, Georgescu RE, Li H, O'Donnell ME
Sci Adv (2024) 10 pp. eadl1739-eadl1739 [ Pubmed: 38427736 DOI: doi:10.1126/sciadv.adl1739 ]
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Pcna clamp (Complex from Saccharomyces cerevisiae)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (37 kDa, Protein from Saccharomyces cerevisiae)
- Elg1 isoform 1 (91 kDa, Protein from Saccharomyces cerevisiae)
- Pcna clamp unloader elg1-rfc (Complex)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Proliferating cell nuclear antigen (29 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Pcna clamp unloader elg1-rfc (Complex from Saccharomyces cerevisiae)
Cryo-EM map of the Saccharomyces cerevisiae clamp unloader Elg1-RFC bound to PCNA
Resolution: 3.25 Å
EM Method: Single-particle
Fitted PDBs: 8thd
Q-score: 0.375
Zheng F, Yao NY, Georgescu RE, Li H, O'Donnell ME
Sci Adv (2024) 10 pp. eadl1739-eadl1739 [ Pubmed: 38427736 DOI: doi:10.1126/sciadv.adl1739 ]
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Pcna clamp (Complex from Saccharomyces cerevisiae)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (37 kDa, Protein from Saccharomyces cerevisiae)
- Elg1 isoform 1 (91 kDa, Protein from Saccharomyces cerevisiae)
- Pcna clamp unloader elg1-rfc (Complex)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Proliferating cell nuclear antigen (29 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Pcna clamp unloader elg1-rfc (Complex from Saccharomyces cerevisiae)
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 8fs3
Q-score: 0.503
Zheng F, Georgescu RE, Yao NY, O'Donnell ME, Li H
bioRxiv (2023) [ DOI: doi:10.1101/2023.05.03.539266 Pubmed: 37205533 ]
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (37 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Template strand (15 kDa, DNA from synthetic construct)
- Rad24-rfc-911 clamp-dna (Complex)
- Primer strand 2 (6 kDa, DNA from synthetic construct)
- Checkpoint protein rad24 (62 kDa, Protein from Saccharomyces cerevisiae)
- Ddc1 isoform 1 (69 kDa, Protein from Saccharomyces cerevisiae)
- Dna damage checkpoint control protein rad17 (45 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex from synthetic construct)
- Dna damage checkpoint control protein mec3 (53 kDa, Protein from Saccharomyces cerevisiae)
- Proteins complex (Complex from Saccharomyces cerevisiae)
Resolution: 2.94 Å
EM Method: Single-particle
Fitted PDBs: 8fs4
Q-score: 0.452
Zheng F, Georgescu RE, Yao NY, O'Donnell ME, Li H
bioRxiv (2023) [ DOI: doi:10.1101/2023.05.03.539266 Pubmed: 37205533 ]
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (37 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Template strand (15 kDa, DNA from synthetic construct)
- Rad24-rfc-911 clamp-dna (Complex)
- Primer strand 2 (6 kDa, DNA from synthetic construct)
- Ddc1 isoform 1 (69 kDa, Protein from Saccharomyces cerevisiae)
- Dna damage checkpoint control protein rad17 (45 kDa, Protein from Saccharomyces cerevisiae)
- Primer strand 1 (6 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex from synthetic construct)
- Checkpoint protein rad24 (62 kDa, Protein from Saccharomyces cerevisiae)
- Dna damage checkpoint control protein mec3 (53 kDa, Protein from Saccharomyces cerevisiae)
- Proteins complex (Complex from Saccharomyces cerevisiae)
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 8fs5
Q-score: 0.548
Zheng F, Georgescu RE, Yao NY, O'Donnell ME, Li H
bioRxiv (2023) [ DOI: doi:10.1101/2023.05.03.539266 Pubmed: 37205533 ]
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (37 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Template strand (15 kDa, DNA from synthetic construct)
- Rad24-rfc-911 clamp-dna (Complex)
- Primer strand 2 (6 kDa, DNA from synthetic construct)
- Checkpoint protein rad24 (62 kDa, Protein from Saccharomyces cerevisiae)
- Ddc1 isoform 1 (69 kDa, Protein from Saccharomyces cerevisiae)
- Dna damage checkpoint control protein rad17 (45 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex from synthetic construct)
- Primer strand 1 (6 kDa, DNA from synthetic construct)
- Dna damage checkpoint control protein mec3 (53 kDa, Protein from Saccharomyces cerevisiae)
- Proteins complex (Complex from Saccharomyces cerevisiae)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8fs6
Q-score: 0.487
Zheng F, Georgescu RE, Yao NY, O'Donnell ME, Li H
bioRxiv (2023) [ DOI: doi:10.1101/2023.05.03.539266 Pubmed: 37205533 ]
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (37 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Template strand (15 kDa, DNA from synthetic construct)
- Rad24-rfc-911 clamp-dna (Complex)
- Primer strand 2 (6 kDa, DNA from synthetic construct)
- Checkpoint protein rad24 (62 kDa, Protein from Saccharomyces cerevisiae)
- Ddc1 isoform 1 (69 kDa, Protein from Saccharomyces cerevisiae)
- Dna damage checkpoint control protein rad17 (45 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex from synthetic construct)
- Primer strand 1 (6 kDa, DNA from synthetic construct)
- Dna damage checkpoint control protein mec3 (53 kDa, Protein from Saccharomyces cerevisiae)
- Proteins complex (Complex from Saccharomyces cerevisiae)
Resolution: 2.85 Å
EM Method: Single-particle
Fitted PDBs: 8fs7
Q-score: 0.542
Zheng F, Georgescu RE, Yao NY, O'Donnell ME, Li H
bioRxiv (2023) [ DOI: doi:10.1101/2023.05.03.539266 Pubmed: 37205533 ]
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (37 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Template strand (15 kDa, DNA from synthetic construct)
- Rad24-rfc-911 clamp-dna (Complex)
- Primer strand 2 (6 kDa, DNA from synthetic construct)
- Checkpoint protein rad24 (62 kDa, Protein from Saccharomyces cerevisiae)
- Ddc1 isoform 1 (69 kDa, Protein from Saccharomyces cerevisiae)
- Dna damage checkpoint control protein rad17 (45 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex from synthetic construct)
- Primer strand 1 (6 kDa, DNA from synthetic construct)
- Dna damage checkpoint control protein mec3 (53 kDa, Protein from Saccharomyces cerevisiae)
- Proteins complex (Complex from Saccharomyces cerevisiae)
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 8fs8
Q-score: 0.446
Zheng F, Georgescu RE, Yao NY, O'Donnell ME, Li H
bioRxiv (2023) [ DOI: doi:10.1101/2023.05.03.539266 Pubmed: 37205533 ]
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Primer strand 1 (6 kDa, DNA from synthetic construct)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Checkpoint protein rad24 (57 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (37 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Template strand (15 kDa, DNA from synthetic construct)
- Rad24-rfc-911 clamp-dna (Complex)
- Primer strand 2 (6 kDa, DNA from synthetic construct)
- Ddc1 isoform 1 (69 kDa, Protein from Saccharomyces cerevisiae)
- Dna damage checkpoint control protein rad17 (45 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex from synthetic construct)
- Dna damage checkpoint control protein mec3 (53 kDa, Protein from Saccharomyces cerevisiae)
- Proteins complex (Complex from Saccharomyces cerevisiae)
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 7tfh
Q-score: 0.469
Zheng F, Georgescu R, Yao NY, Li H, O'Donnell ME
eLife (2022) 11 [ DOI: doi:10.7554/eLife.77469 Pubmed: 35829698 ]
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Dsdna (Complex from synthetic construct)
- Proliferating cell nuclear antigen (29 kDa, Protein from Saccharomyces cerevisiae)
- Primer strand (6 kDa, DNA from synthetic construct)
- Rfc-pcna-dna1-dna2 (Complex)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Template strand (12 kDa, DNA from synthetic construct)
- Replication factor c subunit 1 (95 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (38 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Proteins (Complex from Saccharomyces cerevisiae)