Cryo-EM structure of a nucleolar pre-60S ribosome (Rpf1-TAP)
Resolution: 3.65 Å
EM Method: Single-particle
Fitted PDBs: 5z3g
Q-score: 0.422
Zhou D, Zhu X, Zheng S, Tan D, Dong MQ, Ye K
Protein Cell (2019) 10 pp. 120-130 [ DOI: doi:10.1007/s13238-018-0526-7 Pubmed: 29557065 ]
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae S288c)
- Ribosome biogenesis protein brx1 (33 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae S288c)
- Atp-dependent rna helicase has1 (56 kDa, Protein from Saccharomyces cerevisiae S288c)
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae)
- Ribosome biogenesis protein nsa1 (51 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae S288c)
- Nucleolar protein 7 (69 kDa, Protein from Saccharomyces cerevisiae S288c)
- Rrna-processing protein ebp2 (49 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae S288c)
- Protein mak16 (35 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae S288c)
- Ribosome production factor 1 (35 kDa, Protein from Saccharomyces cerevisiae S288c)
- Ribosomal rna-processing protein 1 (33 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae S288c)
- Unassigned (13 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae S288c)
- 25s rrna (1 MDa, RNA from Saccharomyces cerevisiae)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Saccharomyces cerevisiae S288c)
- Its2 rna (74 kDa, RNA from Saccharomyces cerevisiae)
- Proteasome-interacting protein cic1 (42 kDa, Protein from Saccharomyces cerevisiae S288c)
- Ribosome biogenesis protein 15 (25 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae S288c)
- Ribosome biogenesis protein rlp7 (36 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae S288c)
- Rpf1-tap pre-60s (Complex from Saccharomyces cerevisiae)
Cryo-EM structure of the 90S small subunit pre-ribosome (Dhr1-depleted, Enp1-TAP, state 1)
Resolution: 8.7 Å
EM Method: Single-particle
Fitted PDBs: 5wyj
Q-score: 0.16
Sun Q, Zhu X, Qi J, An W, Lan P, Tan D, Chen R, Wang B, Zheng S, Zhang C, Chen X, Zhang W, Chen J, Dong MQ, Ye K
eLife (2017) 6 [ Pubmed: 28244370 DOI: doi:10.7554/eLife.22086 ]
- 40s ribosomal protein s7-a (21 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s13 (17 kDa, Protein from Baker's yeast)
- 13 kda ribonucleoprotein-associated protein (13 kDa, Protein from Baker's yeast)
- Utp5 (54 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s24-a (15 kDa, Protein from Baker's yeast)
- 18s ribosomal rna (583 kDa, RNA from Baker's yeast)
- 40s ribosomal protein s9-a (22 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s1-a (28 kDa, Protein from Baker's yeast)
- Helical domain protein (137 kDa, Protein from Baker's yeast)
- Utp11 (21 kDa, Protein from Baker's yeast)
- Utp17 (76 kDa, Protein from Baker's yeast)
- Enp2 (60 kDa, Protein from Baker's yeast)
- Utp15 (43 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 13 (91 kDa, Protein from Baker's yeast)
- U3 small nucleolar ribonucleoprotein protein imp3 (21 kDa, Protein from Baker's yeast)
- Periodic tryptophan protein 2 (104 kDa, Protein from Baker's yeast)
- U3 small nucleolar ribonucleoprotein protein imp4 (33 kDa, Protein from Baker's yeast)
- Sof1 (41 kDa, Protein from Baker's yeast)
- Rrna-processing protein fcf1 (21 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 21 (104 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 22 (140 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s8-a (22 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s23-a (16 kDa, Protein from Baker's yeast)
- Helical domain protein (84 kDa, Protein from Baker's yeast)
- Ribosomal rna small subunit methyltransferase nep1 (27 kDa, Protein from Baker's yeast)
- Ribosome biogenesis protein utp30 (31 kDa, Protein from Baker's yeast)
- Pre-rrna-processing protein pno1 (30 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 18 (66 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s6-a (27 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s11-a (17 kDa, Protein from Baker's yeast)
- Mpp10,u3 small nucleolar rna-associated protein mpp10 (54 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s16-a (15 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s28-a (7 kDa, Protein from Baker's yeast)
- Ribosome biogenesis protein bms1 (135 kDa, Protein from Baker's yeast)
- Ribosomal rna-processing protein 7 (34 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s27-a (8 kDa, Protein from Baker's yeast)
- Rna 3'-terminal phosphate cyclase-like protein (40 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 12 (106 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s4-a (29 kDa, Protein from Baker's yeast)
- Unassigned helices (87 kDa, Protein from Baker's yeast)
- 90s small subunit pre-ribosome (dhr1-depleted, enp1-tap) (Complex from Saccharomyces cerevisiae)
- Ubiquitin-40s ribosomal protein s31 (17 kDa, Protein from Baker's yeast)
- Utp8 (60 kDa, Protein from Baker's yeast)
- Essential nuclear protein 1 (55 kDa, Protein from Baker's yeast)
- Krr1 small subunit processome component (37 kDa, Protein from Baker's yeast)
- Utp20 (212 kDa, Protein from Baker's yeast)
- Rrna biogenesis protein rrp5 (193 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s30-a (7 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s5 (25 kDa, Protein from Baker's yeast)
- Utp7 (47 kDa, Protein from Baker's yeast)
- 5ets rna (225 kDa, RNA from Baker's yeast)
- Utp9 (48 kDa, Protein from Baker's yeast)
- Rrna 2'-o-methyltransferase fibrillarin (34 kDa, Protein from Baker's yeast)
- Ribosomal rna-processing protein 9 (65 kDa, Protein from Baker's yeast)
- Nucleolar protein 58 (57 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s22-a (14 kDa, Protein from Baker's yeast)
- U3 rna (106 kDa, RNA from Baker's yeast)
- 40s ribosomal protein s14-a (14 kDa, Protein from Baker's yeast)
- Nucleolar protein 56 (56 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s12 (15 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 10,utp10 (173 kDa, Protein from Baker's yeast)
- Utp4 (66 kDa, Protein from Baker's yeast)