T33-ml28 - Designed Tetrahedral Protein Cage Using Machine Learning Algorithms
Resolution: 2.73 Å
EM Method: Single-particle
Fitted PDBs: 8ui2
Q-score: 0.594
Meador K, Castells-Graells R, Aguirre R, Sawaya MR, Arbing MA, Sherman T, Senarathne C, Yeates TO
Structure (2024) 32 pp. 751-765.e11 [ DOI: doi:10.1016/j.str.2024.02.017 Pubmed: 38513658 ]
- T33-ml28-redesigned-cuta-fold (11 kDa, Protein from synthetic construct)
- T33-ml28-redesigned-tandem-bmc-t-fold (21 kDa, Protein from synthetic construct)
- T33-ml28 designed tetrahedral protein cage using machine learning (400 kDa, Complex from synthetic construct)
T33-ml30 - Designed Tetrahedral Protein Cage Using Machine Learning Algorithms
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 8ukm
Q-score: 0.332
Meador K, Castells-Graells R, Aguirre R, Sawaya MR, Arbing MA, Sherman T, Senarathne C, Yeates TO
Structure (2024) 32 pp. 751-765.e11 [ DOI: doi:10.1016/j.str.2024.02.017 Pubmed: 38513658 ]
- T33-ml30-redesigned-tandem-bmc-t-fold (20 kDa, Protein from synthetic construct)
- T33-ml30-redesigned-4-ot-fold (14 kDa, Protein from synthetic construct)
- T33-ml30 designed tetrahedral protein cage using machine learning (400 kDa, Complex from synthetic construct)
T33-ml35 Assembly Intermediate - Designed Tetrahedral Protein Cage Using Machine Learning Algorithms
Resolution: 4.42 Å
EM Method: Single-particle
Fitted PDBs: 8umr
Q-score: 0.236
Meador K, Castells-Graells R, Aguirre R, Sawaya MR, Arbing MA, Sherman T, Senarathne C, Yeates TO
Structure (2024) 32 pp. 751-765.e11 [ DOI: doi:10.1016/j.str.2024.02.017 Pubmed: 38513658 ]
T33-ml23 Assembly Intermediate - Designed Tetrahedral Protein Cage Using Machine Learning Algorithms
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 8un1
Q-score: 0.256
Meador K, Castells-Graells R, Aguirre R, Sawaya MR, Arbing MA, Sherman T, Senarathne C, Yeates TO
Structure (2024) 32 pp. 751-765.e11 [ DOI: doi:10.1016/j.str.2024.02.017 Pubmed: 38513658 ]
T33-ml23 - Designed Tetrahedral Protein Cage Using Machine Learning Algorithms
Resolution: 2.02 Å
EM Method: Single-particle
Fitted PDBs: 8uf0
Q-score: 0.687
Meador K, Castells-Graells R, Aguirre R, Sawaya MR, Arbing MA, Sherman T, Senarathne C, Yeates TO
Structure (2024) 32 pp. 751-765.e11 [ DOI: doi:10.1016/j.str.2024.02.017 Pubmed: 38513658 ]
- Water (18 Da, Ligand)
- T33-ml23-redesigned-tandem-bmc-t-fold (21 kDa, Protein from synthetic construct)
- T33-ml23-redesigned-cuta-fold (11 kDa, Protein from synthetic construct)
- T33-ml23 designed tetrahedral protein cage using machine learning (400 kDa, Complex from synthetic construct)
T33-ml35 - Designed Tetrahedral Protein Cage Using Machine Learning Algorithms
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 8ump
Q-score: 0.586
Meador K, Castells-Graells R, Aguirre R, Sawaya MR, Arbing MA, Sherman T, Senarathne C, Yeates TO
Structure (2024) 32 pp. 751-765.e11 [ DOI: doi:10.1016/j.str.2024.02.017 Pubmed: 38513658 ]
- T33-ml35-redesigned-tpr-domain-fold (13 kDa, Protein from synthetic construct)
- T33-ml35 designed tetrahedral protein cage using machine learning (400 kDa, Complex from synthetic construct)
- T33-ml35-redesigned-cuta-fold (14 kDa, Protein from synthetic construct)
Cryo-EM imaging scaffold subunits A and B used to display KRAS G12C complex with GDP
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 8g3k
Q-score: 0.668
Castells-Graells R, Meador K, Arbing MA, Sawaya MR, Gee M, Cascio D, Gleave E, Debreczeni JE, Breed J, Leopold K, Patel A, Jahagirdar D, Lyons B, Subramaniam S, Phillips C, Yeates TO
PNAS (2023) 120 pp. e2305494120-e2305494120 [ DOI: doi:10.1073/pnas.2305494120 Pubmed: 37669364 ]
KRAS G12C complex with GDP imaged on a cryo-EM imaging scaffold
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 8g42
Q-score: 0.504
Castells-Graells R, Meador K, Arbing MA, Sawaya MR, Gee M, Cascio D, Gleave E, Debreczeni JE, Breed J, Leopold K, Patel A, Jahagirdar D, Lyons B, Subramaniam S, Phillips C, Yeates TO
PNAS (2023) 120 pp. e2305494120-e2305494120 [ DOI: doi:10.1073/pnas.2305494120 Pubmed: 37669364 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Rcg-33 - cryo-em imaging scaffold subunit b fused to darpin (35 kDa, Protein from synthetic construct)
- Gtpase kras (21 kDa, Protein from Homo sapiens)
- Kras g12c displayed on a cryo-em imaging scaffold (Complex from synthetic construct)
KRAS G12C complex with GDP and AMG 510 imaged on a cryo-EM imaging scaffold
Resolution: 3.19 Å
EM Method: Single-particle
Fitted PDBs: 8g47
Q-score: 0.465
Castells-Graells R, Meador K, Arbing MA, Sawaya MR, Gee M, Cascio D, Gleave E, Debreczeni JE, Breed J, Leopold K, Patel A, Jahagirdar D, Lyons B, Subramaniam S, Phillips C, Yeates TO
PNAS (2023) 120 pp. e2305494120-e2305494120 [ DOI: doi:10.1073/pnas.2305494120 Pubmed: 37669364 ]
- Amg 510 (bound form) (562 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Kras g12c complex with gdp and amg 510 imaged on a cryo-em imaging scaffold (Complex from synthetic construct)
- Rcg-33 - cryo-em imaging scaffold subunit b fused to darpin (35 kDa, Protein from synthetic construct)
- Gtpase kras (21 kDa, Protein from Homo sapiens)
Green Fluorescence Protein imaged on a cryo-EM imaging scaffold
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 8g4e
Q-score: 0.566
Castells-Graells R, Meador K, Arbing MA, Sawaya MR, Gee M, Cascio D, Gleave E, Debreczeni JE, Breed J, Leopold K, Patel A, Jahagirdar D, Lyons B, Subramaniam S, Phillips C, Yeates TO
PNAS (2023) 120 pp. e2305494120-e2305494120 [ DOI: doi:10.1073/pnas.2305494120 Pubmed: 37669364 ]