Cryo-EM Structure of primitive cyanobacterium photosystem I
Resolution: 2.04 Å
EM Method: Single-particle
Fitted PDBs: 7f4v
Q-score: 0.752
Kato K, Hamaguchi T, Nagao R, Kawakami K, Ueno Y, Suzuki T, Uchida H, Murakami A, Nakajima Y, Yokono M, Akimoto S, Dohmae N, Yonekura K, Shen JR
bioRxiv (2022) [ DOI: doi:10.1101/2021.09.29.462462 ]
- Photosystem i reaction center subunit iv (7 kDa, Protein from Gloeobacter violaceus (strain ATCC 29082 / PCC 7421))
- Photosystem i p700 chlorophyll a apoprotein a1 (86 kDa, Protein from Gloeobacter violaceus (strain ATCC 29082 / PCC 7421))
- Photosystem i reaction center subunit z (3 kDa, Protein from Gloeobacter violaceus (strain ATCC 29082 / PCC 7421))
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Photosystem i reaction center subunit iii (19 kDa, Protein from Gloeobacter violaceus (strain ATCC 29082 / PCC 7421))
- Photosystem i reaction center subunit xii (3 kDa, Protein from Gloeobacter violaceus (strain ATCC 29082 / PCC 7421))
- Photosystem i iron-sulfur center (8 kDa, Protein from Gloeobacter violaceus (strain ATCC 29082 / PCC 7421))
- Menaquinone-4 (444 Da, Ligand)
- Beta-carotene (536 Da, Ligand)
- Photosystem i reaction center subunit ii (15 kDa, Protein from Gloeobacter violaceus (strain ATCC 29082 / PCC 7421))
- Psi trimer (1 MDa, Complex from Gloeobacter violaceus PCC 7421)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Water (18 Da, Ligand)
- Chlorophyll a isomer (893 Da, Ligand)
- Unknown protein (2 kDa, Protein from Gloeobacter violaceus (strain ATCC 29082 / PCC 7421))
- Photosystem i p700 chlorophyll a apoprotein a2 (96 kDa, Protein from Gloeobacter violaceus (strain ATCC 29082 / PCC 7421))
- Iron/sulfur cluster (351 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- Photosystem i reaction center subunit xi (15 kDa, Protein from Gloeobacter violaceus (strain ATCC 29082 / PCC 7421))
Structure of Wild-type PSI monomer1 from Cyanophora paradoxa
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7dr0
Q-score: 0.53
Kato K, Nagao R, Ueno Y, Yokono M, Suzuki T, Jiang TY, Dohmae N, Akita F, Akimoto S, Miyazaki N, Shen JR
bioRxiv (2022) [ DOI: doi:10.1101/2022.01.03.474851 ]
- Photosystem i reaction center subunit viii (3 kDa, Protein from Cyanophora paradoxa)
- Phylloquinone (450 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a2 (82 kDa, Protein from Cyanophora paradoxa)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Photosystem i reaction center subunit ii, cyanelle (23 kDa, Protein from Cyanophora paradoxa)
- Photosystem i reaction center subunit xi (15 kDa, Protein from Cyanophora paradoxa)
- Beta-carotene (536 Da, Ligand)
- Photosystem i reaction center subunit psak 1 (15 kDa, Protein from Cyanophora paradoxa)
- Photosystem i reaction center subunit iii (20 kDa, Protein from Cyanophora paradoxa)
- Photosystem i p700 chlorophyll a apoprotein a1 (83 kDa, Protein from Cyanophora paradoxa)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Psi monomer1 (350 kDa, Complex from Cyanophora paradoxa)
- Chlorophyll a isomer (893 Da, Ligand)
- Photosystem i reaction center subunit iv (8 kDa, Protein from Cyanophora paradoxa)
- Photosystem i reaction center subunit ix (4 kDa, Protein from Cyanophora paradoxa)
- Photosystem i iron-sulfur center (8 kDa, Protein from Cyanophora paradoxa)
- Iron/sulfur cluster (351 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- Photosystem i reaction center subunit xii (3 kDa, Protein from Cyanophora paradoxa)
Structure of Wild-type PSI monomer2 from Cyanophora paradoxa
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7dr1
Q-score: 0.525
Kato K, Nagao R, Ueno Y, Yokono M, Suzuki T, Jiang TY, Dohmae N, Akita F, Akimoto S, Miyazaki N, Shen JR
bioRxiv (2022) [ DOI: doi:10.1101/2022.01.03.474851 ]
- Photosystem i reaction center subunit viii (3 kDa, Protein from Cyanophora paradoxa)
- Phylloquinone (450 Da, Ligand)
- Photosystem i iron-sulfur center (8 kDa, Protein from Cyanophora paradoxa)
- Chlorophyll a (893 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a2 (82 kDa, Protein from Cyanophora paradoxa)
- Chlorophyll a isomer (893 Da, Ligand)
- Photosystem i reaction center subunit iii (20 kDa, Protein from Cyanophora paradoxa)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Photosystem i reaction center subunit ii, cyanelle (23 kDa, Protein from Cyanophora paradoxa)
- Photosystem i p700 chlorophyll a apoprotein a1 (83 kDa, Protein from Cyanophora paradoxa)
- Photosystem i reaction center subunit iv (8 kDa, Protein from Cyanophora paradoxa)
- Psi monomer2 (350 kDa, Complex from Cyanophora paradoxa)
- Photosystem i reaction center subunit xii (3 kDa, Protein from Cyanophora paradoxa)
- Iron/sulfur cluster (351 Da, Ligand)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Photosystem i reaction center subunit ix (4 kDa, Protein from Cyanophora paradoxa)
- Photosystem i reaction center subunit xi (15 kDa, Protein from Cyanophora paradoxa)
- Beta-carotene (536 Da, Ligand)
Structure of Wild-type PSI tetramer from Cyanophora paradoxa
Kato K, Nagao R, Ueno Y, Yokono M, Suzuki T, Jiang TY, Dohmae N, Akita F, Akimoto S, Miyazaki N, Shen JR
bioRxiv (2022) [ DOI: doi:10.1101/2022.01.03.474851 ]
- Psi tetramer (1 MDa, Complex from Cyanophora paradoxa)
Structure of GraFix PSI tetramer from Cyanophora paradoxa
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7dr2
Q-score: 0.374
Kato K, Nagao R, Ueno Y, Yokono M, Suzuki T, Jiang TY, Dohmae N, Akita F, Akimoto S, Miyazaki N, Shen JR
bioRxiv (2022) [ DOI: doi:10.1101/2022.01.03.474851 ]
- Photosystem i reaction center subunit viii (3 kDa, Protein from Cyanophora paradoxa)
- Phylloquinone (450 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a2 (82 kDa, Protein from Cyanophora paradoxa)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Photosystem i reaction center subunit ii, cyanelle (23 kDa, Protein from Cyanophora paradoxa)
- Photosystem i reaction center subunit xi (15 kDa, Protein from Cyanophora paradoxa)
- Beta-carotene (536 Da, Ligand)
- Photosystem i reaction center subunit psak 1 (15 kDa, Protein from Cyanophora paradoxa)
- Photosystem i reaction center subunit iii (20 kDa, Protein from Cyanophora paradoxa)
- Photosystem i p700 chlorophyll a apoprotein a1 (83 kDa, Protein from Cyanophora paradoxa)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Psi tetramer (1 MDa, Complex from Cyanophora paradoxa)
- Chlorophyll a isomer (893 Da, Ligand)
- Photosystem i reaction center subunit iv (8 kDa, Protein from Cyanophora paradoxa)
- Photosystem i reaction center subunit ix (4 kDa, Protein from Cyanophora paradoxa)
- Photosystem i iron-sulfur center (8 kDa, Protein from Cyanophora paradoxa)
- Iron/sulfur cluster (351 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- Photosystem i reaction center subunit xii (3 kDa, Protein from Cyanophora paradoxa)
Structure of PSI-isiA supercomplex from Thermosynechococcus vulcanus
Resolution: 2.74 Å
EM Method: Single-particle
Fitted PDBs: 6k33
Q-score: 0.445
Akita F, Nagao R, Kato K, Nakajima Y, Yokono M, Ueno Y, Suzuki T, Dohmae N, Shen JR, Akimoto S, Miyazaki N
Commun Biol (2020) 3 pp. 232-232 [ DOI: doi:10.1038/s42003-020-0949-6 Pubmed: 32393811 ]
- Photosystem i reaction center subunit iv (8 kDa, Protein from Thermosynechococcus vulcanus)
- Phylloquinone (450 Da, Ligand)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a2 (82 kDa, Protein from Thermosynechococcus vulcanus)
- Photosystem i reaction center subunit viii (4 kDa, Protein from Thermosynechococcus vulcanus)
- Beta-carotene (536 Da, Ligand)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Water (18 Da, Ligand)
- Iron stress in-duced protein a (39 kDa, Protein from Thermosynechococcus vulcanus)
- Photosystem i p700 chlorophyll a apoprotein a1 (83 kDa, Protein from Thermosynechococcus vulcanus)
- Chlorophyll a isomer (893 Da, Ligand)
- Photosystem i iron-sulfur center (8 kDa, Protein from Thermosynechococcus vulcanus)
- Photosystem i reaction center subunit iii (17 kDa, Protein from Thermosynechococcus vulcanus)
- Photosystem i reaction center subunit xi (16 kDa, Protein from Thermosynechococcus vulcanus)
- Calcium ion (40 Da, Ligand)
- Photosystem i reaction center subunit psak (8 kDa, Protein from Thermosynechococcus vulcanus)
- Photosystem i reaction center subunit xii (3 kDa, Protein from Thermosynechococcus vulcanus)
- Photosystem i reaction center subunit ii (15 kDa, Protein from Thermosynechococcus vulcanus)
- Psi-isia (1 MDa, Complex from Thermosynechococcus vulcanus)
- Photosystem i reaction center subunit ix (4 kDa, Protein from Thermosynechococcus vulcanus)
- Photosystem i reaction center subunit psax (3 kDa, Protein from Thermosynechococcus vulcanus)
- Iron/sulfur cluster (351 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
Structure of PSI tetramer from Anabaena
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6jeo
Q-score: 0.516
Kato K, Nagao R, Jiang TY, Ueno Y, Yokono M, Chan SK, Watanabe M, Ikeuchi M, Shen JR, Akimoto S, Miyazaki N, Akita F
Nat Commun (2019) 10 pp. 4929-4929 [ Pubmed: 31666526 DOI: doi:10.1038/s41467-019-12942-8 ]
- Iron/sulfur cluster (351 Da, Ligand)
- Photosystem i reaction center subunit viii (5 kDa, Protein from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576))
- Phylloquinone (450 Da, Ligand)
- Photosystem i reaction center subunit iv (7 kDa, Protein from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576))
- Beta-carotene (536 Da, Ligand)
- Photosystem i reaction center subunit ii (15 kDa, Protein from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576))
- Photosystem i 4.8 kda protein (4 kDa, Protein from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576))
- Psi tetramer (1 MDa, Complex from Nostoc sp. PCC 7120)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Photosystem i iron-sulfur center (8 kDa, Protein from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576))
- Photosystem i p700 chlorophyll a apoprotein a1 (83 kDa, Protein from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576))
- Photosystem i reaction center subunit iii (17 kDa, Protein from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576))
- Photosystem i reaction center subunit xi (18 kDa, Protein from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576))
- Chlorophyll a isomer (893 Da, Ligand)
- Photosystem i reaction center subunit xii (4 kDa, Protein from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576))
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Photosystem i reaction center subunit ix (5 kDa, Protein from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576))
- Photosystem i p700 chlorophyll a apoprotein a2 1 (83 kDa, Protein from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576))
- Chlorophyll a (893 Da, Ligand)
- Photosystem i reaction center subunit psak 1 (8 kDa, Protein from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576))
Structure of PSI dimer from Anabaena
Kato K, Nagao R, Jiang TY, Ueno Y, Yokono M, Chan SK, Watanabe M, Ikeuchi M, Shen JR, Akimoto S, Miyazaki N, Akita F
Nat Commun (2019) 10 pp. 4929-4929 [ Pubmed: 31666526 DOI: doi:10.1038/s41467-019-12942-8 ]
- Psi dimer (700 kDa, Complex from Nostoc sp. PCC 7120)
Cryo-EM map of Chlamydomonas reinhardtii PSI-LHCI supercomplex at 6.9A resolution
Kubota-Kawai H, Burton-Smith RN, Tokutsu R, Song C, Akimoto S, Yokono M, Ueno Y, Kim E, Watanabe A, Murata K, Minagawa J
J Biol Chem (2019) 294 pp. 4304-4314 [ Pubmed: 30670590 DOI: doi:10.1074/jbc.RA118.006536 ]