Cryo-EM structure of the PGE2-bound EP2-Gs complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7cx2
Q-score: 0.536
Qu C, Mao C, Xiao P, Shen Q, Zhong YN, Yang F, Shen DD, Tao X, Zhang H, Yan X, Zhao RJ, He J, Guan Y, Zhang C, Hou G, Zhang PJ, Hou G, Li Z, Yu X, Chai RJ, Guan YF, Sun JP, Zhang Y
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abf1268 Pubmed: 33811074 ]
- Water (18 Da, Ligand)
- Nb35 (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Prostaglandin e2 receptor ep2 subtype (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Pge2-bound ep2-gs complex (Complex)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- (z)-7-[(1r,2r,3r)-3-hydroxy-2-[(e,3s)-3-hydroxyoct-1-enyl]-5-oxo-cyclopentyl]hept-5-enoic acid (352 Da, Ligand)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Ep2 (Complex from Homo sapiens)
- Gs (Complex from Homo sapiens)
Cryo-EM structure of the Taprenepag-bound EP2-Gs complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7cx3
Q-score: 0.554
Qu C, Mao C, Xiao P, Shen Q, Zhong YN, Yang F, Shen DD, Tao X, Zhang H, Yan X, Zhao RJ, He J, Guan Y, Zhang C, Hou G, Zhang PJ, Hou G, Li Z, Yu X, Chai RJ, Guan YF, Sun JP, Zhang Y
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abf1268 Pubmed: 33811074 ]
- Nb35 (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Prostaglandin e2 receptor ep2 subtype (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Taprenepag-bound ep2-gs complex (Complex)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- 2-[3-[[(4-pyrazol-1-ylphenyl)methyl-pyridin-3-ylsulfonyl-amino]methyl]phenoxy]ethanoic acid (478 Da, Ligand)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Ep2 (Complex from Homo sapiens)
- Gs (Complex from Homo sapiens)
Cryo-EM structure of the Evatanepag-bound EP2-Gs complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7cx4
Q-score: 0.543
Qu C, Mao C, Xiao P, Shen Q, Zhong YN, Yang F, Shen DD, Tao X, Zhang H, Yan X, Zhao RJ, He J, Guan Y, Zhang C, Hou G, Zhang PJ, Hou G, Li Z, Yu X, Chai RJ, Guan YF, Sun JP, Zhang Y
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abf1268 Pubmed: 33811074 ]
- 2-[3-[[(4-~{tert}-butylphenyl)methyl-pyridin-3-ylsulfonyl-amino]methyl]phenoxy]ethanoic acid (468 Da, Ligand)
- Nb35 (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Prostaglandin e2 receptor ep2 subtype (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Pge2-bound ep2-gs complex (Complex)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Ep2 (Complex from Homo sapiens)
- Gs (Complex from Homo sapiens)