Complex of RecF and DNA from Thermus thermophilus.
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8a8j
Q-score: 0.556
Nirwal S, Czarnocki-Cieciura M, Chaudhary A, Zajko W, Skowronek K, Chamera S, Figiel M, Nowotny M
Nat Struct Mol Biol (2023) 30 pp. 650-660 [ DOI: doi:10.1038/s41594-023-00967-z Pubmed: 37081315 ]
- Complex of recf and dna from thermus thermophilus. (95 kDa, Complex from Thermus thermophilus HB8)
- Oligo2 (12 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna replication and repair protein recf (37 kDa, Protein from Thermus thermophilus HB8)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Oligo1 (7 kDa, DNA from synthetic construct)
Complex of RecF-RecR-DNA from Thermus thermophilus.
Resolution: 3.05 Å
EM Method: Single-particle
Fitted PDBs: 8a93
Q-score: 0.525
Nirwal S, Czarnocki-Cieciura M, Chaudhary A, Zajko W, Skowronek K, Chamera S, Figiel M, Nowotny M
Nat Struct Mol Biol (2023) 30 pp. 650-660 [ DOI: doi:10.1038/s41594-023-00967-z Pubmed: 37081315 ]
- Dna replication and repair protein recf (37 kDa, Protein from Thermus thermophilus HB8)
- Recombination protein recr (21 kDa, Protein from Thermus thermophilus HB8)
- Oligo2 (12 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Complex of recf-recr-dna from thermus thermophilus. (180 kDa, Complex from Thermus thermophilus HB8)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Oligo1 (7 kDa, DNA from synthetic construct)
Complex of RecO-RecR-DNA from Thermus thermophilus.
Resolution: 6.09 Å
EM Method: Single-particle
Fitted PDBs: 8ab0
Q-score: 0.233
Nirwal S, Czarnocki-Cieciura M, Chaudhary A, Zajko W, Skowronek K, Chamera S, Figiel M, Nowotny M
Nat Struct Mol Biol (2023) 30 pp. 650-660 [ DOI: doi:10.1038/s41594-023-00967-z Pubmed: 37081315 ]
- Zinc ion (65 Da, Ligand)
- Dna repair protein reco (24 kDa, Protein from Thermus thermophilus HB8)
- Recombination protein recr (21 kDa, Protein from Thermus thermophilus HB8)
- Complex of reco-recr-dna from thermus thermophilus. (130 kDa, Complex from Thermus thermophilus HB8)
- Oligo2 (12 kDa, DNA from synthetic construct)
- Oligo1 (7 kDa, DNA from synthetic construct)
Complex of RecF-RecO-RecR-DNA from Thermus thermophilus (low resolution reconstruction).
Resolution: 3.65 Å
EM Method: Single-particle
Fitted PDBs: 8bpr
Q-score: 0.359
Nirwal S, Czarnocki-Cieciura M, Chaudhary A, Zajko W, Skowronek K, Chamera S, Figiel M, Nowotny M
Nat Struct Mol Biol (2023) 30 pp. 650-660 [ DOI: doi:10.1038/s41594-023-00967-z Pubmed: 37081315 ]
- Complex of recf-reco-recr-dna from thermus thermophilus. (205 kDa, Complex from Thermus thermophilus HB8)
- Zinc ion (65 Da, Ligand)
- Dna repair protein reco (24 kDa, Protein from Thermus thermophilus HB8)
- Recombination protein recr (21 kDa, Protein from Thermus thermophilus HB8)
- Oligo2 (12 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna replication and repair protein recf (37 kDa, Protein from Thermus thermophilus HB8)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Oligo1 (7 kDa, DNA from synthetic construct)
Abortive infection DNA polymerase AbiK from Lactococcus lactis
Resolution: 2.27 Å
EM Method: Single-particle
Fitted PDBs: 7r06
Q-score: 0.676
Figiel M, Gapinska M, Czarnocki-Cieciura M, Zajko W, Sroka M, Skowronek K, Nowotny M
Nucleic Acids Res (2022) 50 pp. 10026-10040 [ Pubmed: 36107766 DOI: doi:10.1093/nar/gkac772 ]
- Abortive infection protein abik (Complex from Lactococcus lactis)
- Homohexamer of abik with single-stranded dna covalently attached to each of the subunits (420 kDa, Complex)
- Dna (5'-d(*cp*cp*cp*cp*cp*cp*cp*cp*cp*cp*cp*c)-3') (Complex from Escherichia coli)
- Abik (71 kDa, Protein from Lactococcus lactis)
- Dna (5'-d(*cp*cp*cp*cp*cp*cp*cp*cp*cp*cp*cp*c)-3') (3 kDa, DNA from Escherichia coli)
Abortive infection DNA polymerase AbiK from Lactococcus lactis, Y44F variant
Resolution: 2.68 Å
EM Method: Single-particle
Fitted PDBs: 7z0z
Q-score: 0.614
Figiel M, Gapinska M, Czarnocki-Cieciura M, Zajko W, Sroka M, Skowronek K, Nowotny M
Nucleic Acids Res (2022) 50 pp. 10026-10040 [ Pubmed: 36107766 DOI: doi:10.1093/nar/gkac772 ]
- Homohexamer of l. lactis abik y44f mutant (420 kDa, Complex from Lactococcus lactis)
- Abik (71 kDa, Protein from Lactococcus lactis)
Cryo-EM structure of E. coli TnsB in complex with right end fragment of Tn7 transposon
Resolution: 2.68 Å
EM Method: Single-particle
Fitted PDBs: 7pik
Q-score: 0.586
Kaczmarska Z, Czarnocki-Cieciura M, Gorecka-Minakowska KM, Wingo RJ, Jackiewicz J, Zajko W, Poznanski JT, Rawski M, Grant T, Peters JE, Nowotny M
Mol Cell (2022) 82 pp. 2618- [ Pubmed: 35654042 DOI: doi:10.1016/j.molcel.2022.05.005 ]
- Tnsb (Complex from Escherichia coli)
- Tn7 transposon right end fragment (Complex from Escherichia coli)
- Transposon tn7 transposition protein tnsb (81 kDa, Protein from Escherichia coli)
- Right end fragment of tn7 transposon (21 kDa, DNA from Escherichia coli)
- Tnsb-dna complex (43 kDa, Complex)
- Right end fragment of tn7 transposon (21 kDa, DNA from Escherichia coli)
Cryo-EM helical reconstruction of E. coli TnsB in complex with right end fragment of Tn7 transposon
Kaczmarska Z, Czarnocki-Cieciura M, Gorecka-Minakowska KM, Wingo RJ, Jackiewicz J, Zajko W, Poznanski JT, Rawski M, Grant T, Peters JE, Nowotny M
Mol Cell (2022) 82 pp. 2618-2632.e7 [ Pubmed: 35654042 DOI: doi:10.1016/j.molcel.2022.05.005 ]
- Tnsb (Complex from Escherichia coli)
- Tn7 transposon right end fragment (Complex from Escherichia coli)
- Tnsb-dna complex (43 kDa, Complex)
- Right end fragment of tn7 transposon chain 2 (DNA from Escherichia coli)
- Right end fragment of tn7 transposon chain 1 (DNA from Escherichia coli)
- Transposon tn7 transposition protein tnsb (Protein from Escherichia coli)