Cryo-EM reveals cholesterol binding in the lysosomal GPCR-like protein LYCHOS
Zhao J, Shen Q, Yong X, Li X, Tian X, Sun S, Xu Z, Zhang X, Zhang L, Yang H, Shao Z, Xu H, Jiang Y, Zhang Y, Yan W
Nat Struct Mol Biol (2025) [ Pubmed: 39824976 DOI: doi:10.1038/s41594-024-01470-9 ]
- Lysosomal cholesterol signaling protein (93 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Cholesterol-bound lychos (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- [(2r)-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] octadecanoate (720 Da, Ligand)
- Sodium ion (22 Da, Ligand)
Cryo-EM structure of the C terminal region of PTX3 with a section of coiled-coil
Shah A, Zhang X, Snee M, Lockhart-Cairns MP, Levy CW, Jowitt TA, Birchenough HL, Dean L, Collins R, Dodd RJ, Roberts ARE, Enghild JJ, Mantovani A, Fontana J, Baldock C, Inforzato A, Richter RP, Day AJ
Matrix Biol. (2025) [ Pubmed: 39814214 DOI: doi:10.1016/j.matbio.2025.01.002 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Pentraxin-related protein ptx3 (40 kDa, Protein from Homo sapiens)
- Pentraxin 3 (321 kDa, Complex from Homo sapiens)
- Water (18 Da, Ligand)
BA.2.86 RBD protein in complex with ACE2.
Resolution: 3.29 Å
EM Method: Single-particle
Fitted PDBs: 8yzb
Q-score: 0.477
Liu Y, Zhao X, Shi J, Wang Y, Liu H, Hu YF, Hu B, Shuai H, Yuen TT, Chai Y, Liu F, Gong HR, Li J, Wang X, Jiang S, Zhang X, Zhang Y, Li X, Wang L, Hartnoll M, Zhu T, Hou Y, Huang X, Yoon C, Wang Y, He Y, Zhou M, Du L, Zhang X, Chan WM, Chen LL, Cai JP, Yuan S, Zhou J, Huang JD, Yuen KY, To KK, Chan JF, Zhang BZ, Sun L, Wang P, Chu H
Nat Commun (2024) 15 pp. 8728-8728 [ Pubmed: 39379369 DOI: doi:10.1038/s41467-024-53033-7 ]
- Ba.2.86 rbd protein in complex with ace2 (Complex from Homo sapiens)
- Spike glycoprotein,fibritin,fusion protein (142 kDa, Protein from synthetic construct)
- Angiotensin-converting enzyme 2 (85 kDa, Protein from Homo sapiens)
Structure of BA.2.86 spike protein in complex with ACE2.
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8yzc
Q-score: 0.267
Liu Y, Zhao X, Shi J, Wang Y, Liu H, Hu YF, Hu B, Shuai H, Yuen TT, Chai Y, Liu F, Gong HR, Li J, Wang X, Jiang S, Zhang X, Zhang Y, Li X, Wang L, Hartnoll M, Zhu T, Hou Y, Huang X, Yoon C, Wang Y, He Y, Zhou M, Du L, Zhang X, Chan WM, Chen LL, Cai JP, Yuan S, Zhou J, Huang JD, Yuen KY, To KK, Chan JF, Zhang BZ, Sun L, Wang P, Chu H
Nat Commun (2024) 15 pp. 8728-8728 [ DOI: doi:10.1038/s41467-024-53033-7 Pubmed: 39379369 ]
Structure of JN.1 RBD protein in complex with ACE2.
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 8yzd
Q-score: 0.573
Liu Y, Zhao X, Shi J, Wang Y, Liu H, Hu YF, Hu B, Shuai H, Yuen TT, Chai Y, Liu F, Gong HR, Li J, Wang X, Jiang S, Zhang X, Zhang Y, Li X, Wang L, Hartnoll M, Zhu T, Hou Y, Huang X, Yoon C, Wang Y, He Y, Zhou M, Du L, Zhang X, Chan WM, Chen LL, Cai JP, Yuan S, Zhou J, Huang JD, Yuen KY, To KK, Chan JF, Zhang BZ, Sun L, Wang P, Chu H
Nat Commun (2024) 15 pp. 8728-8728 [ Pubmed: 39379369 DOI: doi:10.1038/s41467-024-53033-7 ]
The JN.1 spike protein (S) in complex with ACE2.
Resolution: 3.06 Å
EM Method: Single-particle
Fitted PDBs: 8yze
Q-score: 0.25
Liu Y, Zhao X, Shi J, Wang Y, Liu H, Hu YF, Hu B, Shuai H, Yuen TT, Chai Y, Liu F, Gong HR, Li J, Wang X, Jiang S, Zhang X, Zhang Y, Li X, Wang L, Hartnoll M, Zhu T, Hou Y, Huang X, Yoon C, Wang Y, He Y, Zhou M, Du L, Zhang X, Chan WM, Chen LL, Cai JP, Yuan S, Zhou J, Huang JD, Yuen KY, To KK, Chan JF, Zhang BZ, Sun L, Wang P, Chu H
Nat Commun (2024) 15 pp. 8728-8728 [ DOI: doi:10.1038/s41467-024-53033-7 Pubmed: 39379369 ]
Haspin bound to nucleosome in position 1
Resolution: 3.01 Å
EM Method: Single-particle
Fitted PDBs: 9b2s
Q-score: 0.536
Hicks CW, Gliech CR, Zhang X, Rahman S, Vasquez S, Holland AJ, Wolberger C
To Be Published
- Histone h2a (14 kDa, Protein from Xenopus laevis)
- 601 dna (185-mer) (56 kDa, DNA from synthetic construct)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Serine/threonine-protein kinase haspin (40 kDa, Protein from Homo sapiens)
- 601 dna (185-mer) (57 kDa, DNA from synthetic construct)
- Haspin bound to nucleosome (Complex from Xenopus laevis)
Haspin bound to nucleosome in position 2
Hicks CW, Gliech CR, Zhang X, Rahman S, Vasquez S, Holland AJ, Wolberger C
To Be Published
- 601 dna (185-mer) (56 kDa, DNA from synthetic construct)
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- Histone h2a (14 kDa, Protein from Xenopus laevis)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Haspin bound to nucleosome (Complex from Xenopus laevis)
- 601 dna (185-mer) (57 kDa, DNA from synthetic construct)
- Serine/threonine-protein kinase haspin (40 kDa, Protein from Homo sapiens)
Resolution: 3.64 Å
EM Method: Single-particle
Fitted PDBs: 9b2u
Q-score: 0.363
Hicks CW, Gliech CR, Zhang X, Rahman S, Vasquez S, Holland AJ, Wolberger C
To Be Published
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- Serine/threonine-protein kinase haspin (40 kDa, Protein from Homo sapiens)
- Haspin bound to h3 tail (Complex from Xenopus laevis)
Structure of rat beta-arrestin 1 by fiducial-assisted cryo-EM
Resolution: 3.52 Å
EM Method: Single-particle
Fitted PDBs: 9dng
Q-score: 0.431
Kahsai AW, Pakharukova N, Kwon HY, Shah KS, Liang-Lin JG, Del Real CT, Shim PJ, Lee MA, Ngo VA, Shreiber BN, Liu S, Schwalb AM, Espinoza EF, Thomas BN, Kunzle CA, Smith JS, Wang J, Kim J, Zhang X, Rockman HA, Thomsen ARB, Rein LAM, Shi L, Ahn S, Masoudi A, Lefkowitz RJ
bioRxiv (2024) [ Pubmed: 39763753 DOI: doi:10.1101/2024.12.27.630464 ]
- Beta-arrestin 1 with insertion of soluble cytochrome b562 bound to anti-bril fab (Complex from Rattus norvegicus)
- Anti-bril fab heavy chain (23 kDa, Protein from Homo sapiens)
- Beta-arrestin-1,soluble cytochrome b562 (55 kDa, Protein from Rattus norvegicus)
- Anti-bril fab light chain (23 kDa, Protein from Homo sapiens)