Cryo-EM Structure of KdpFABC in E1 state
Sweet ME, Larsen C, Zhang X, Schlame M, Pedersen BP, Stokes DL
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2105195118 Pubmed: 34272288 ]
- Potassium-transporting atpase kdpc subunit (Protein from Escherichia coli K-12)
- Kdpfabc in e1 apo state (160 kDa, Complex from Escherichia coli K-12)
- Potassium-transporting atpase atp-binding subunit (Protein from Escherichia coli K-12)
- Potassium-transporting atpase kdpf subunit (Protein from Escherichia coli K-12)
- Potassium-transporting atpase potassium-binding subunit (Protein from Escherichia coli K-12)
Cryo-EM Structure of KdpFABC in E2Pi state with AlF4
Sweet ME, Larsen C, Zhang X, Schlame M, Pedersen BP, Stokes DL
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2105195118 Pubmed: 34272288 ]
- Kdpfabc in e2pi state with alf4 in sample buffer (160 kDa, Complex from Escherichia coli K-12)
- Potassium-transporting atpase kdpc subunit (Protein from Escherichia coli K-12)
- Potassium-transporting atpase atp-binding subunit (Protein from Escherichia coli K-12)
- Potassium-transporting atpase kdpf subunit (Protein from Escherichia coli K-12)
- Potassium-transporting atpase potassium-binding subunit (Protein from Escherichia coli K-12)
CryoEM Structure of KdpFABC in E2Pi state with AlF4
Sweet ME, Larsen C, Zhang X, Schlame M, Pedersen BP, Stokes DL
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2105195118 Pubmed: 34272288 ]
- Potassium-transporting atpase kdpc subunit (Protein from Escherichia coli K-12)
- Kdpfabc in e2pi state with alf4 and k+ in sample buffer (160 kDa, Complex from Escherichia coli K-12)
- Potassium-transporting atpase atp-binding subunit (Protein from Escherichia coli K-12)
- Potassium-transporting atpase kdpf subunit (Protein from Escherichia coli K-12)
- Potassium-transporting atpase potassium-binding subunit (Protein from Escherichia coli K-12)
CryoEM Structure of KdpFABC in E2Pi state with VO4
Sweet ME, Larsen C, Zhang X, Schlame M, Pedersen BP, Stokes DL
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2105195118 Pubmed: 34272288 ]
- Potassium-transporting atpase kdpc subunit (Protein from Escherichia coli K-12)
- Potassium-transporting atpase atp-binding subunit (Protein from Escherichia coli K-12)
- Kdpfabc in e2pi state with vo4 in sample buffer (160 kDa, Complex from Escherichia coli K-12)
- Potassium-transporting atpase kdpf subunit (Protein from Escherichia coli K-12)
- Potassium-transporting atpase potassium-binding subunit (Protein from Escherichia coli K-12)
CryoEM Structure of KdpFABC in E1 state
Sweet ME, Larsen C, Zhang X, Schlame M, Pedersen BP, Stokes DL
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2105195118 Pubmed: 34272288 ]
- Potassium-transporting atpase kdpc subunit (Protein from Escherichia coli K-12)
- Potassium-transporting atpase atp-binding subunit (Protein from Escherichia coli K-12)
- Kdpfabc in e1 apo state with vo4 in sample buffer (160 kDa, Complex from Escherichia coli K-12)
- Potassium-transporting atpase kdpf subunit (Protein from Escherichia coli K-12)
- Potassium-transporting atpase potassium-binding subunit (Protein from Escherichia coli K-12)
Cryo-EM Structure of KdpFABC in E2Pi state with VO4 and K+
Sweet ME, Larsen C, Zhang X, Schlame M, Pedersen BP, Stokes DL
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2105195118 Pubmed: 34272288 ]
- Potassium-transporting atpase kdpc subunit (Protein from Escherichia coli K-12)
- Kdpfabc in e2pi state with vo4 and k+ in sample buffer (160 kDa, Complex from Escherichia coli K-12)
- Potassium-transporting atpase atp-binding subunit (Protein from Escherichia coli K-12)
- Potassium-transporting atpase kdpf subunit (Protein from Escherichia coli K-12)
- Potassium-transporting atpase potassium-binding subunit (Protein from Escherichia coli K-12)
CryoEM Structure of KdpFABC in E1 state
Sweet ME, Larsen C, Zhang X, Schlame M, Pedersen BP, Stokes DL
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2105195118 Pubmed: 34272288 ]
- Kdpfabc in e1 state with vo4 and k+ in sample buffer (160 kDa, Complex from Escherichia coli K-12)
- Potassium-transporting atpase kdpc subunit (Protein from Escherichia coli K-12)
- Potassium-transporting atpase atp-binding subunit (Protein from Escherichia coli K-12)
- Potassium-transporting atpase kdpf subunit (Protein from Escherichia coli K-12)
- Potassium-transporting atpase potassium-binding subunit (Protein from Escherichia coli K-12)
Cryo-EM Structure of KdpFABC in E1ATP state with AMP-PCP
Sweet ME, Larsen C, Zhang X, Schlame M, Pedersen BP, Stokes DL
PNAS (2021) 118 [ Pubmed: 34272288 DOI: doi:10.1073/pnas.2105195118 ]
- Potassium-transporting atpase kdpf subunit (Protein from Escherichia coli K-12)
- Potassium-transporting atpase potassium-binding subunit (Protein from Escherichia coli K-12)
- Kdpfabc in e1atp state with amp-pcp in sample buffer (160 kDa, Complex from Escherichia coli K-12)
- Potassium-transporting atpase kdpc subunit (Protein from Escherichia coli K-12)
- Potassium-transporting atpase atp-binding subunit (Protein from Escherichia coli K-12)
Cryo-EM Structure of KdpFABC in E2Pi state with BeF3
Sweet ME, Larsen C, Zhang X, Schlame M, Pedersen BP, Stokes DL
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2105195118 Pubmed: 34272288 ]
- Potassium-transporting atpase kdpc subunit (Protein from Escherichia coli K-12)
- Kdpfabc in e2pi state with bef3 in sample buffer (160 kDa, Complex from Escherichia coli K-12)
- Potassium-transporting atpase atp-binding subunit (Protein from Escherichia coli K-12)
- Potassium-transporting atpase kdpf subunit (Protein from Escherichia coli K-12)
- Potassium-transporting atpase potassium-binding subunit (Protein from Escherichia coli K-12)
Cryo-EM Structure of KdpFABC in E2Pi state with MgF4
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7bgy
Q-score: 0.566
Sweet ME, Larsen C, Zhang X, Schlame M, Pedersen BP, Stokes DL
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2105195118 Pubmed: 34272288 ]
- Water (18 Da, Ligand)
- (1s)-2-{[(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (720 Da, Ligand)
- Potassium-transporting atpase kdpf subunit (3 kDa, Protein from Escherichia coli K-12)
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli K-12)
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Tetrafluoromagnesate(2-) (100 Da, Ligand)
- Potassium-transporting atpase kdpc subunit (22 kDa, Protein from Escherichia coli K-12)
- (2r)-3-(((2-aminoethoxy)(hydroxy)phosphoryl)oxy)-2-(palmitoyloxy)propyl (e)-octadec-9-enoate (717 Da, Ligand)
- Kdpfabc in e2pi state with mgf4 (160 kDa, Complex from Escherichia coli K-12)