Cryo-EM structure of a Zinc-loaded wild-type YiiP-Fab complex
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8f6e
Q-score: 0.412
Hussein A, Fan S, Lopez-Redondo M, Kenney I, Zhang X, Beckstein O, Stokes DL
eLife (2023) 12 [ Pubmed: 37906094 DOI: doi:10.7554/eLife.87167 ]
- Fab light chain (23 kDa, Protein from Homo sapiens)
- Wild-type zinc-loaded yiip-fab complex (163 kDa, Complex from Shewanella oneidensis)
- Fab heavy chain (25 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Cadmium and zinc efflux pump fief (32 kDa, Protein from Shewanella oneidensis)
Cryo-EM structure of a Zinc-loaded D51A mutant of the YiiP-Fab complex
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8f6f
Q-score: 0.448
Hussein A, Fan S, Lopez-Redondo M, Kenney I, Zhang X, Beckstein O, Stokes DL
eLife (2023) 12 [ Pubmed: 37906094 DOI: doi:10.7554/eLife.87167 ]
- D51a zinc-loaded yiip-fab complex (163 kDa, Complex from Shewanella oneidensis)
- Fab2r heavy chain (25 kDa, Protein from Homo sapiens)
- Cadmium and zinc efflux pump fief (32 kDa, Protein from Shewanella oneidensis)
- Fab2r light chain (23 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
Cryo-EM structure of a Zinc-loaded asymmetrical TMD D70A mutant of the YiiP-Fab complex
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 8f6h
Q-score: 0.419
Hussein A, Fan S, Lopez-Redondo M, Kenney I, Zhang X, Beckstein O, Stokes DL
eLife (2023) 12 [ Pubmed: 37906094 DOI: doi:10.7554/eLife.87167 ]
- Cadmium and zinc efflux pump fief (32 kDa, Protein from Shewanella oneidensis MR-1)
- Fab2r heavy chain (25 kDa, Protein from Homo sapiens)
- D70a asymmetrical zinc-loaded yiip-fab complex (163 kDa, Complex from Shewanella oneidensis)
- Fab2r light chain (23 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
Cryo-EM structure of a Zinc-loaded symmetrical D70A mutant of the YiiP-Fab complex
Resolution: 4.03 Å
EM Method: Single-particle
Fitted PDBs: 8f6i
Q-score: 0.388
Hussein A, Fan S, Lopez-Redondo M, Kenney I, Zhang X, Beckstein O, Stokes DL
eLife (2023) 12 [ Pubmed: 37906094 DOI: doi:10.7554/eLife.87167 ]
- Cadmium and zinc efflux pump fief (32 kDa, Protein from Shewanella oneidensis MR-1)
- Fab2r heavy chain (25 kDa, Protein from Homo sapiens)
- D70a symmetrical zinc-loaded yiip-fab complex (163 kDa, Complex from Shewanella oneidensis)
- Fab2r light chain (23 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
Cryo-EM structure of a Zinc-loaded D287A mutant of the YiiP-Fab complex
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8f6j
Q-score: 0.408
Hussein A, Fan S, Lopez-Redondo M, Kenney I, Zhang X, Beckstein O, Stokes DL
eLife (2023) 12 [ Pubmed: 37906094 DOI: doi:10.7554/eLife.87167 ]
- D287a zinc-loaded yiip-fab complex (163 kDa, Complex from Shewanella oneidensis)
- Fab2r heavy chain (25 kDa, Protein from Homo sapiens)
- Fab2r light chain (23 kDa, Protein from Homo sapiens)
- Cadmium and zinc efflux pump fief (32 kDa, Protein from Shewanella oneidensis)
- Zinc ion (65 Da, Ligand)
Cryo-EM structure of a Zinc-loaded H263A/D287A mutant of the YiiP-Fab complex
Resolution: 3.46 Å
EM Method: Single-particle
Fitted PDBs: 8f6k
Q-score: 0.433
Hussein A, Fan S, Lopez-Redondo M, Kenney I, Zhang X, Beckstein O, Stokes DL
eLife (2023) 12 [ Pubmed: 37906094 DOI: doi:10.7554/eLife.87167 ]
- Cadmium and zinc efflux pump fief (32 kDa, Protein from Shewanella oneidensis)
- D287a_h263a zinc-loaded yiip-fab complex (325 kDa, Complex from Shewanella oneidensis)
- Zinc ion (65 Da, Ligand)
Cryo-EM Structure of KdpFABC in E2Pi state with MgF4
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7bgy
Q-score: 0.566
Sweet ME, Larsen C, Zhang X, Schlame M, Pedersen BP, Stokes DL
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2105195118 Pubmed: 34272288 ]
- Water (18 Da, Ligand)
- (1s)-2-{[(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (720 Da, Ligand)
- Potassium-transporting atpase kdpf subunit (3 kDa, Protein from Escherichia coli K-12)
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli K-12)
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Tetrafluoromagnesate(2-) (100 Da, Ligand)
- Potassium-transporting atpase kdpc subunit (22 kDa, Protein from Escherichia coli K-12)
- (2r)-3-(((2-aminoethoxy)(hydroxy)phosphoryl)oxy)-2-(palmitoyloxy)propyl (e)-octadec-9-enoate (717 Da, Ligand)
- Kdpfabc in e2pi state with mgf4 (160 kDa, Complex from Escherichia coli K-12)
Cryo-EM Structure of KdpFABC in E1 state with K
Resolution: 3.38 Å
EM Method: Single-particle
Fitted PDBs: 7bh1
Q-score: 0.461
Sweet ME, Larsen C, Zhang X, Schlame M, Pedersen BP, Stokes DL
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2105195118 Pubmed: 34272288 ]
- Water (18 Da, Ligand)
- Kdpfabc in e1 apo state (160 kDa, Complex from Escherichia coli K-12)
- Potassium-transporting atpase kdpf subunit (3 kDa, Protein from Escherichia coli K-12)
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli K-12)
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli K-12)
- Potassium ion (39 Da, Ligand)
- Potassium-transporting atpase kdpc subunit (22 kDa, Protein from Escherichia coli K-12)
- (2r)-3-(((2-aminoethoxy)(hydroxy)phosphoryl)oxy)-2-(palmitoyloxy)propyl (e)-octadec-9-enoate (717 Da, Ligand)
Cryo-EM Structure of KdpFABC in E2Pi state with BeF3 and K+
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7bh2
Q-score: 0.559
Sweet ME, Larsen C, Zhang X, Schlame M, Pedersen BP, Stokes DL
PNAS (2021) 118 [ Pubmed: 34272288 DOI: doi:10.1073/pnas.2105195118 ]
- Potassium-transporting atpase kdpf subunit (3 kDa, Protein from Escherichia coli K-12)
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli K-12)
- Potassium-transporting atpase kdpc subunit (22 kDa, Protein from Escherichia coli K-12)
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli K-12)
- Beryllium trifluoride ion (66 Da, Ligand)
- Water (18 Da, Ligand)
- Kdpfabc in e2pi state with bef3 and k+ (160 kDa, Complex from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- (2r)-3-(((2-aminoethoxy)(hydroxy)phosphoryl)oxy)-2-(palmitoyloxy)propyl (e)-octadec-9-enoate (717 Da, Ligand)
CryoEM Structure of KdpFABC in E1-ATP state
Resolution: 3.23 Å
EM Method: Single-particle
Fitted PDBs: 7lc3
Q-score: 0.473
Sweet ME, Larsen C, Zhang X, Schlame M, Pedersen BP, Stokes DL
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2105195118 Pubmed: 34272288 ]
- Water (18 Da, Ligand)
- Potassium-transporting atpase kdpc subunit (22 kDa, Protein from Escherichia coli (strain K12))
- Potassium ion (39 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli (strain K12))
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli (strain K12))
- Phosphomethylphosphonic acid adenylate ester (505 Da, Ligand)
- Potassium-transporting atpase kdpf subunit (3 kDa, Protein from Escherichia coli (strain K12))
- Kdpfabc in e1-atp state (160 kDa, Complex from Escherichia coli (strain K12))
- (2r)-3-(((2-aminoethoxy)(hydroxy)phosphoryl)oxy)-2-(palmitoyloxy)propyl (e)-octadec-9-enoate (717 Da, Ligand)