Structure of rat beta-arrestin 1 by fiducial-assisted cryo-EM
Resolution: 3.52 Å
EM Method: Single-particle
Fitted PDBs: 9dng
Q-score: 0.431
Kahsai AW, Pakharukova N, Kwon HY, Shah KS, Liang-Lin JG, Del Real CT, Shim PJ, Lee MA, Ngo VA, Shreiber BN, Liu S, Schwalb AM, Espinoza EF, Thomas BN, Kunzle CA, Smith JS, Wang J, Kim J, Zhang X, Rockman HA, Thomsen ARB, Rein LAM, Shi L, Ahn S, Masoudi A, Lefkowitz RJ
bioRxiv (2024) [ DOI: doi:10.1101/2024.12.27.630464 Pubmed: 39763753 ]
- Beta-arrestin-1,soluble cytochrome b562 (55 kDa, Protein from Rattus norvegicus)
- Anti-bril fab light chain (23 kDa, Protein from Homo sapiens)
- Anti-bril fab heavy chain (23 kDa, Protein from Homo sapiens)
- Beta-arrestin 1 with insertion of soluble cytochrome b562 bound to anti-bril fab (Complex from Rattus norvegicus)
Structure of rat beta-arrestin 1 bound to allosteric inhibitor
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 9dnm
Q-score: 0.466
Kahsai AW, Pakharukova N, Kwon HY, Shah KS, Liang-Lin JG, Del Real CT, Shim PJ, Lee MA, Ngo VA, Shreiber BN, Liu S, Schwalb AM, Espinoza EF, Thomas BN, Kunzle CA, Smith JS, Wang J, Kim J, Zhang X, Rockman HA, Thomsen ARB, Rein LAM, Shi L, Ahn S, Masoudi A, Lefkowitz RJ
bioRxiv (2024) [ DOI: doi:10.1101/2024.12.27.630464 Pubmed: 39763753 ]
- (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14r,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one (366 Da, Ligand)
- Beta-arrestin-1,soluble cytochrome b562 (55 kDa, Protein from Rattus norvegicus)
- Beta-arrestin 1 with insertion of soluble cytochrome b562 bound to anti-bril fab (Complex from Rattus norvegicus)
- Anti-bril fab light chain (23 kDa, Protein from Homo sapiens)
- Anti-bril fab heavy chain (23 kDa, Protein from Homo sapiens)
Unaligned multi-frame micrographs of the mu opioid receptor bound to a nanobody antagonist NbE
Yu J, Kumar A, Zhang X, Martin C, Van Holsbeeck K, Raia P, Koehl A, Laeremans T, Steyaert J, Manglik A, Ballet S, Boland A, Stoeber M
Nat Commun (2024) 15 [ DOI: 10.1038/s41467-024-52947-6 Pubmed: 39384768 ]
Image sets:
Structure of Src in complex with beta-arrestin 1 revealing SH3 binding sites
Resolution: 3.34 Å
EM Method: Single-particle
Fitted PDBs: 9bt8
Q-score: 0.539
Pakharukova N, Thomas BN, Bansia H, Li L, Abzalimov RR, Kim J, Kahsai AW, Pani B, Bassford DK, Liu S, Zhang X, des Georges A, Lefkowitz RJ
bioRxiv (2024) [ Pubmed: 39131402 DOI: doi:10.1101/2024.07.31.605623 ]
- Antibody fragment fab30, heavy chain (25 kDa, Protein from Bacteriophage sp.)
- Nanobody 32 (13 kDa, Protein from Lama glama)
- Antibody fragment fab30, light chain (23 kDa, Protein from Bacteriophage sp.)
- Vasopressin v2 receptor (3 kDa, Protein from Homo sapiens)
- Beta-arrestin 1 bound to a g protein-coupled receptor phosphopeptide, nanobody 32 and antibody fragment fab30 in complex with three-domain src (sh3-sh2-sh1). (Complex from Rattus norvegicus)
- Beta-arrestin-1 (44 kDa, Protein from Rattus norvegicus)
- Proto-oncogene tyrosine-protein kinase src (54 kDa, Protein from Gallus gallus)
Structure of SH3 domain of Src in complex with beta-arrestin 1
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 9cx3
Q-score: 0.423
Pakharukova N, Thomas BN, Bansia H, Li L, Abzalimov RR, Kim J, Kahsai AW, Pani B, Bassford DK, Liu S, Zhang X, des Georges A, Lefkowitz RJ
bioRxiv (2024) [ Pubmed: 39131402 DOI: doi:10.1101/2024.07.31.605623 ]
- Nanobody 32 (13 kDa, Protein from Lama glama)
- Vasopressin v2 receptor (3 kDa, Protein from Homo sapiens)
- Beta-arrestin-1 (44 kDa, Protein from Rattus norvegicus)
- Proto-oncogene tyrosine-protein kinase src (9 kDa, Protein from Gallus gallus)
- Beta-arrestin 1 bound to a g protein-coupled receptor phosphopeptide, nanobody 32 and antibody fragment fab30 in complex with sh3 domain of src (Complex from Rattus norvegicus)
- Antibody fragment fab30, light chain (23 kDa, Protein from Mus musculus)
- Antibody fragment fab30, heavy chain (25 kDa, Protein from Mus musculus)
Structure of SH3 domain of Src in complex with beta-arrestin 1
Resolution: 3.34 Å
EM Method: Single-particle
Fitted PDBs: 9cx9
Q-score: 0.47
Pakharukova N, Thomas BN, Bansia H, Li L, Abzalimov RR, Kim J, Kahsai AW, Pani B, Bassford DK, Liu S, Zhang X, des Georges A, Lefkowitz RJ
bioRxiv (2024) [ Pubmed: 39131402 DOI: doi:10.1101/2024.07.31.605623 ]
- Vasopressin v2 receptor (3 kDa, Protein from Homo sapiens)
- Beta-arrestin 1 bound to a g protein-coupled receptor phosphopeptide and antibody fragment fab30 in complex with sh3 domain of src (Complex from Rattus norvegicus)
- Proto-oncogene tyrosine-protein kinase src (9 kDa, Protein from Gallus gallus)
- Beta-arrestin-1 (44 kDa, Protein from Rattus norvegicus)
- Antibody fragment fab30, light chain (23 kDa, Protein from Mus musculus)
- Antibody fragment fab30, heavy chain (25 kDa, Protein from Mus musculus)
Cryo-EM structure of the FIGNL1 AAA hexamer bound to RAD51
Carver A, Yu TY, Yates LA, White T, Wang R, Lister K, Jasin M, Zhang X
Science (2025) 387 pp. 426-431 [ Pubmed: 39636933 DOI: doi:10.1126/science.adr7920 ]
- Magnesium ion (24 Da, Ligand)
- Dna repair protein rad51 homolog 1 (37 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Fignl1 hexamer bound to the n-terminus of rad51 (190 kDa, Complex from Homo sapiens)
- Fidgetin-like protein 1 (46 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
Representative tomogram of Enterococcus faecium WT Com15
Klupt S, Fam KT, Zhang X, Chodisetti PK, Mehmood A, Boyd T, Grotjahn D, Park D, Hang HC
eLife (2024) 13 [ Pubmed: 38857064 DOI: doi:10.7554/eLife.95297 ]
Representative tomogram of Enterococcus faecium SagA complementation strain
Klupt S, Fam KT, Zhang X, Chodisetti PK, Mehmood A, Boyd T, Grotjahn D, Park D, Hang HC
eLife (2024) 13 [ Pubmed: 38857064 DOI: doi:10.7554/eLife.95297 ]
Representative tomogram of Enterococcus faecium SagA deletion strain
Klupt S, Fam KT, Zhang X, Chodisetti PK, Mehmood A, Boyd T, Grotjahn D, Park D, Hang HC
eLife (2024) 13 [ Pubmed: 38857064 DOI: doi:10.7554/eLife.95297 ]