Cryo-EM structure of the DHA bound FFA4-Gq complex
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 8t3q
Q-score: 0.467
Zhang X, Guseinov AA, Jenkins L, Li K, Tikhonova IG, Milligan G, Zhang C
Sci Adv (2024) 10 pp. eadj2384-eadj2384 [ DOI: doi:10.1126/sciadv.adj2384 Pubmed: 38198545 ]
- Docosa-4,7,10,13,16,19-hexaenoic acid (328 Da, Ligand)
- Dha-ffa4-minigq complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(q) (26 kDa, Protein from Homo sapiens)
- Free fatty acid receptor 4 (34 kDa, Protein from Homo sapiens)
Cryo-EM structure of the Butyrate bound FFA2-Gq complex
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 8t3s
Q-score: 0.509
Zhang X, Guseinov AA, Jenkins L, Li K, Tikhonova IG, Milligan G, Zhang C
Sci Adv (2024) 10 pp. eadj2384-eadj2384 [ DOI: doi:10.1126/sciadv.adj2384 Pubmed: 38198545 ]
- Free fatty acid receptor 2 (29 kDa, Protein from Homo sapiens)
- Butyrate-ffa2-minigq complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(q) subunit alpha (26 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Scfv16 (25 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Butanoic acid (88 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
Cryo-EM structure of the DHA bound FFA1-Gq complex
Resolution: 3.39 Å
EM Method: Single-particle
Fitted PDBs: 8t3v
Q-score: 0.336
Zhang X, Guseinov AA, Jenkins L, Li K, Tikhonova IG, Milligan G, Zhang C
Sci Adv (2024) 10 pp. eadj2384-eadj2384 [ DOI: doi:10.1126/sciadv.adj2384 Pubmed: 38198545 ]
- Free fatty acid receptor 1 (29 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(q) subunit alpha (26 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Mus musculus)
- Dha-ffa1-minigq complex (Complex from Homo sapiens)
- Docosa-4,7,10,13,16,19-hexaenoic acid (328 Da, Ligand)
Cryo-EM structure of the TUG-891 bound FFA4-Gq complex
Resolution: 3.06 Å
EM Method: Single-particle
Fitted PDBs: 8t3o
Q-score: 0.511
Zhang X, Guseinov AA, Jenkins L, Li K, Tikhonova IG, Milligan G, Zhang C
Sci Adv (2024) 10 pp. eadj2384-eadj2384 [ Pubmed: 38198545 DOI: doi:10.1126/sciadv.adj2384 ]
- Guanine nucleotide-binding protein g(q) (26 kDa, Protein from Homo sapiens)
- Tug-891-ffa4-minigq complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- 3-{4-[(4-fluoro-4'-methyl[1,1'-biphenyl]-2-yl)methoxy]phenyl}propanoic acid (364 Da, Ligand)
- (2r)-1-{[(r)-hydroxy{[(1r,2r,3r,4r,5s,6r)-2,3,5,6-tetrahydroxy-4-(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}-3-(octadecanoyloxy)propan-2-yl (5z,8z,11z,14z)-icosa-5,8,11,14-tetraenoate (967 Da, Ligand)
- Free fatty acid receptor 4 (33 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Scfv16 (28 kDa, Protein from Mus musculus)
Cryo-EM structure of the DHA bound FFA1-Gq complex(mask on receptor)
Zhang X, Guseinov AA, Jenkins L, Li K, Tikhonova IG, Milligan G, Zhang C
Sci Adv (2024) 10 pp. eadj2384-eadj2384 [ DOI: doi:10.1126/sciadv.adj2384 Pubmed: 38198545 ]