In situ structure of cytoplasmic microtubule of Chlamydomonas reinhardtii
Chakraborty S, Obr M, Zhang X, Kelley R, Khavnekar S, Waltz F, Righetto RD, Engel B, Kotecha A
To Be Published
In situ clathrin subtomogram average from Chlamydomonas reinhardtii
Kelley R, Khavnekar S, Righetto RD, Heebner JE, Zhang X, Obr M, Chakraborty S, Tagiltsev G, Michael AK, van Dorst S, Waltz F, McCafferty CL, Lamm L, Van der Stappen P, van den Hoek H, Wietrzynski W, Korir PK, Weyand S, Chareshneu A, Briggs JAG, Plitzko J, Engel BD, Kotecha A
To Be Published
The cryo-EM map of TIC-TIEA complex
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8k59
Q-score: 0.337
Zhang KN, Liu Y, Chen M, Wang Y, Lin W, Li M, Zhang X, Gao Y, Gong Q, Chen H, Steve M, Li S, Zhang K, Liu B
To Be Published
- The cryo-em map of tic-tiea complex (400 kDa, Complex from Escherichia coli K-12)
- Dna (63-mer) (19 kDa, DNA from Escherichia coli K-12)
- Rna (5'-r(*ap*gp*gp*up*a)-3') (1 kDa, RNA from Escherichia coli K-12)
- Dna-directed rna polymerase subunit alpha (25 kDa, Protein from Escherichia coli K-12)
- Dna (61-mer) (18 kDa, DNA from Escherichia coli K-12)
- Dna-directed rna polymerase subunit omega (8 kDa, Protein from Escherichia coli K-12)
- Rna polymerase sigma factor rpod (60 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta' (150 kDa, Protein from Escherichia coli K-12)
- 15 kda rna polymerase-binding protein (14 kDa, Protein from Escherichia phage T4)
Structure of SH3 domain of Src in complex with beta-arrestin 1
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 9cx3
Q-score: 0.423
Pakharukova N, Thomas BN, Bansia H, Li L, Abzalimov RR, Kim J, Kahsai AW, Pani B, Bassford DK, Liu S, Zhang X, des Georges A, Lefkowitz RJ
bioRxiv (2024) [ Pubmed: 39131402 DOI: doi:10.1101/2024.07.31.605623 ]
- Nanobody 32 (13 kDa, Protein from Lama glama)
- Vasopressin v2 receptor (3 kDa, Protein from Homo sapiens)
- Beta-arrestin-1 (44 kDa, Protein from Rattus norvegicus)
- Proto-oncogene tyrosine-protein kinase src (9 kDa, Protein from Gallus gallus)
- Beta-arrestin 1 bound to a g protein-coupled receptor phosphopeptide, nanobody 32 and antibody fragment fab30 in complex with sh3 domain of src (Complex from Rattus norvegicus)
- Antibody fragment fab30, light chain (23 kDa, Protein from Mus musculus)
- Antibody fragment fab30, heavy chain (25 kDa, Protein from Mus musculus)
Structure of SH3 domain of Src in complex with beta-arrestin 1
Resolution: 3.34 Å
EM Method: Single-particle
Fitted PDBs: 9cx9
Q-score: 0.47
Pakharukova N, Thomas BN, Bansia H, Li L, Abzalimov RR, Kim J, Kahsai AW, Pani B, Bassford DK, Liu S, Zhang X, des Georges A, Lefkowitz RJ
bioRxiv (2024) [ Pubmed: 39131402 DOI: doi:10.1101/2024.07.31.605623 ]
- Vasopressin v2 receptor (3 kDa, Protein from Homo sapiens)
- Beta-arrestin 1 bound to a g protein-coupled receptor phosphopeptide and antibody fragment fab30 in complex with sh3 domain of src (Complex from Rattus norvegicus)
- Proto-oncogene tyrosine-protein kinase src (9 kDa, Protein from Gallus gallus)
- Beta-arrestin-1 (44 kDa, Protein from Rattus norvegicus)
- Antibody fragment fab30, light chain (23 kDa, Protein from Mus musculus)
- Antibody fragment fab30, heavy chain (25 kDa, Protein from Mus musculus)
In situ PSII subtomogram average from Chlamydomonas reinhardtii
Kelley R, Khavnekar S, Righetto RD, Heebner JE, Zhang X, Obr M, Chakraborty S, Tagiltsev G, Michael AK, van Dorst S, Waltz F, McCafferty CL, Lamm L, Van der Stappen P, van den Hoek H, Wietrzynski W, Korir PK, Weyand S, Chareshneu A, Briggs JAG, Plitzko J, Engel BD, Kotecha A
To Be Published
Cryo-EM structure of the FIGNL1 AAA hexamer bound to RAD51
Carver A, Yu TY, Yates LA, White T, Wang R, Lister K, Jasin M, Zhang X
Science (2025) 387 pp. 426-431 [ Pubmed: 39636933 DOI: doi:10.1126/science.adr7920 ]
- Magnesium ion (24 Da, Ligand)
- Dna repair protein rad51 homolog 1 (37 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Fignl1 hexamer bound to the n-terminus of rad51 (190 kDa, Complex from Homo sapiens)
- Fidgetin-like protein 1 (46 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
In situ nucleosome subtomogram average from Chlamydomonas reinhardtii
Kelley R, Khavnekar S, Righetto RD, Heebner JE, Zhang X, Obr M, Chakraborty S, Tagiltsev G, Michael AK, van Dorst S, Waltz F, McCafferty CL, Lamm L, Van der Stappen P, van den Hoek H, Wietrzynski W, Korir PK, Weyand S, Chareshneu A, Briggs JAG, Plitzko J, Engel BD, Kotecha A
To Be Published
Resolution: 2.9 Å
EM Method: Helical reconstruction
Fitted PDBs: 8seh
Q-score: 0.553
Fernandez A, Hoq MR, Hallinan GI, Li D, Bharath SR, Vago FS, Zhang X, Ozcan KA, Newell KL, Garringer HJ, Jiang W, Ghetti B, Vidal R
Nat Struct Mol Biol (2024) 31 pp. 903-909 [ Pubmed: 38553642 DOI: doi:10.1038/s41594-024-01252-3 ]
- Microtubule-associated protein tau (7 kDa, Protein from Homo sapiens)
- Helical filaments (Tissue from Homo sapiens)