Cryo-EM structure of the FIGNL1 AAA hexamer bound to RAD51
Carver A, Yu TY, Yates LA, White T, Wang R, Lister K, Jasin M, Zhang X
Science (2025) 387 pp. 426-431 [ Pubmed: 39636933 DOI: doi:10.1126/science.adr7920 ]
- Dna repair protein rad51 homolog 1 (37 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Fidgetin-like protein 1 (46 kDa, Protein from Homo sapiens)
- Fignl1 hexamer bound to the n-terminus of rad51 (190 kDa, Complex from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8re4
Q-score: 0.47
Gao F, Ye F, Zhang B, Cronin N, Buck M, Zhang X
PNAS (2024) 121 pp. e2309670120-e2309670120 [ Pubmed: 38170755 DOI: doi:10.1073/pnas.2309670120 ]
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Rna (5'-r(p*gp*cp*cp*gp*c)-3') (1 kDa, RNA from Klebsiella oxytoca)
- Dna-directed rna polymerase subunit beta' (152 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit omega (8 kDa, Protein from Escherichia coli K-12)
- Dna (47-mer) (14 kDa, DNA from Klebsiella oxytoca)
- Magnesium ion (24 Da, Ligand)
- Rna polymerase-sigma54 initial transcribing complex - 5nt pre-translocated complex (Complex from Escherichia coli K-12)
- Rna polymerase sigma-54 factor (42 kDa, Protein from Klebsiella oxytoca)
- Dna (50-mer) (15 kDa, DNA from Klebsiella oxytoca)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Escherichia coli K-12)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8rea
Q-score: 0.427
Gao F, Ye F, Zhang B, Cronin N, Buck M, Zhang X
PNAS (2024) 121 pp. e2309670120-e2309670120 [ Pubmed: 38170755 DOI: doi:10.1073/pnas.2309670120 ]
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Rna polymerase-sigma54 initial transcribing complex - 5nt post-translocated complex (Complex from Escherichia coli K-12)
- Rna (5'-r(p*gp*cp*cp*gp*c)-3') (1 kDa, RNA from Klebsiella oxytoca)
- Dna-directed rna polymerase subunit beta' (152 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit omega (8 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Dna (44-mer) (13 kDa, DNA from Klebsiella oxytoca)
- Rna polymerase sigma-54 factor (42 kDa, Protein from Klebsiella oxytoca)
- Dna (51-mer) (15 kDa, DNA from Klebsiella oxytoca)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Escherichia coli K-12)
Cryo-EM structure of bacterial RNA polymerase-sigma54 initial transcribing complex - 6nt complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8reb
Q-score: 0.108
Gao F, Ye F, Zhang B, Cronin N, Buck M, Zhang X
PNAS (2024) 121 pp. e2309670120-e2309670120 [ Pubmed: 38170755 DOI: doi:10.1073/pnas.2309670120 ]
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Escherichia coli K-12)
- Dna (43-mer) (13 kDa, DNA from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Rna polymerase-sigma54 initial transcribing complex - 5nt post-translocated complex (Complex from Escherichia coli K-12)
- Rna (5'-r(p*gp*cp*cp*gp*cp*g)-3') (1 kDa, RNA from Klebsiella oxytoca)
- Dna-directed rna polymerase subunit omega (8 kDa, Protein from Escherichia coli K-12)
- Rna polymerase sigma-54 factor (39 kDa, Protein from Klebsiella oxytoca)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (152 kDa, Protein from Escherichia coli K-12)
- Dna (52-mer) (16 kDa, DNA from Klebsiella oxytoca)
Cryo-EM structure of bacterial RNA polymerase-sigma54 initial transcribing complex - 7nt complex
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8rec
Q-score: 0.406
Gao F, Ye F, Zhang B, Cronin N, Buck M, Zhang X
PNAS (2024) 121 pp. e2309670120-e2309670120 [ Pubmed: 38170755 DOI: doi:10.1073/pnas.2309670120 ]
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta' (152 kDa, Protein from Escherichia coli K-12)
- Rna polymerase-sigma54 initial transcribing complex - 7nt complex (Complex from Escherichia coli K-12)
- Dna (51-mer) (15 kDa, DNA from Klebsiella oxytoca)
- Dna-directed rna polymerase subunit omega (8 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Dna (46-mer) (14 kDa, DNA from Klebsiella oxytoca)
- Rna polymerase sigma-54 factor (39 kDa, Protein from Klebsiella oxytoca)
- Zinc ion (65 Da, Ligand)
- Rna (5'-r(p*gp*cp*cp*gp*cp*gp*a)-3') (2 kDa, RNA from Klebsiella oxytoca)
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Escherichia coli K-12)
Cryo-EM structure of bacterial RNA polymerase-sigma54 initial transcribing complex - 8nt complex
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 8red
Q-score: 0.387
Gao F, Ye F, Zhang B, Cronin N, Buck M, Zhang X
PNAS (2024) 121 pp. e2309670120-e2309670120 [ Pubmed: 38170755 DOI: doi:10.1073/pnas.2309670120 ]
- Rna (5'-r(p*gp*cp*cp*gp*cp*gp*ap*u)-3') (2 kDa, RNA from Klebsiella oxytoca)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Dna (46-mer) (14 kDa, DNA from Klebsiella oxytoca)
- Rna polymerase-sigma54 initial transcribing complex - 8nt complex (Complex from Escherichia coli K-12)
- Rna polymerase sigma-54 factor (36 kDa, Protein from Klebsiella oxytoca)
- Dna-directed rna polymerase subunit omega (8 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (152 kDa, Protein from Escherichia coli K-12)
- Dna (51-mer) (15 kDa, DNA from Klebsiella oxytoca)
Cryo-EM structure of bacterial RNA polymerase-sigma54 initial transcribing complex - 9nt complex
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8ree
Q-score: 0.136
Gao F, Ye F, Zhang B, Cronin N, Buck M, Zhang X
PNAS (2024) 121 pp. e2309670120-e2309670120 [ Pubmed: 38170755 DOI: doi:10.1073/pnas.2309670120 ]
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Rna (5'-r(p*gp*cp*cp*gp*cp*gp*ap*up*c)-3') (2 kDa, RNA from Klebsiella oxytoca)
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Escherichia coli K-12)
- Dna (49-mer) (15 kDa, DNA from Klebsiella oxytoca)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit omega (8 kDa, Protein from Escherichia coli K-12)
- Rna polymerase sigma-54 factor (36 kDa, Protein from Klebsiella oxytoca)
- Dna (45-mer) (13 kDa, DNA from Klebsiella oxytoca)
- Magnesium ion (24 Da, Ligand)
- Rna polymerase-sigma54 initial transcribing complex - 9nt complex (Complex from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta' (152 kDa, Protein from Escherichia coli K-12)
Cryo-EM structure of the human INO80 complex bound to a WT nucleosome
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7zi4
Q-score: 0.462
Vance NR, Ayala R, Willhoft O, Tvardovskiy A, McCormack EA, Bartke T, Zhang X, Wigley DB
To Be Published
- Ruvb-like 1 (50 kDa, Protein from Homo sapiens)
- Histone h2b type 1-j (13 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Actin-related protein 5 (68 kDa, Protein from Homo sapiens)
- Histone h2a type 1-b/e (14 kDa, Protein from Homo sapiens)
- Dna (158-mer) (48 kDa, DNA from synthetic construct)
- Ino80 complex subunit c (20 kDa, Protein from Homo sapiens)
- Dna (158-mer) (48 kDa, DNA from synthetic construct)
- Ruvb-like 2 (51 kDa, Protein from Homo sapiens)
- Chromatin-remodeling atpase ino80 (177 kDa, Protein from Homo sapiens)
- Ino80 complex subunit b (38 kDa, Protein from Homo sapiens)
- Beryllium trifluoride ion (66 Da, Ligand)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Complex of human ino80core bound to a wt nucleosome (1 MDa, Complex from Homo sapiens)
Structure of Native, iodinated bovine thyroglobulin solved on strepavidin affinity grids.
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7qtq
Q-score: 0.502
Marechal N, Serrano BP, Zhang X, Weitz CJ
Nat Commun (2022) 13 pp. 2380-2380 [ Pubmed: 35501346 DOI: doi:10.1038/s41467-022-30082-4 ]
PF 06882961 bound to the glucagon-like peptide-1 receptor (GLP-1R):Gs complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7lci
Q-score: 0.428
Zhang X, Johnson RM, Drulyte I, Yu L, Kotecha A, Danev R, Wootten D, Sexton PM, Belousoff MJ
Structure (2021) 29 pp. 963- [ Pubmed: 33957078 DOI: doi:10.1016/j.str.2021.04.008 ]
- Glp-1:gs complex with small molecule agonist bound (pf-06882961) (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- 2-[(4-{6-[(4-cyano-2-fluorophenyl)methoxy]pyridin-2-yl}piperidin-1-yl)methyl]-1-{[(2s)-oxetan-2-yl]methyl}-1h-benzimidazole-6-carboxylic acid (555 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)