Resolution: 4.7 Å
EM Method: Single-particle
Fitted PDBs: 6i52
Q-score: 0.101
Yates LA, Aramayo RJ, Pokhrel N, Caldwell CC, Kaplan JA, Perera RL, Spies M, Antony E, Zhang X
Nat Commun (2018) 9 pp. 5447-5447 [ DOI: doi:10.1038/s41467-018-07883-7 Pubmed: 30575763 ]
- Replication factor a protein 2 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Replication factor a protein 1 (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Replication protein a heterotrimeric complex with ssdna (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Replication factor a protein 3 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Replication protein a heterotrimeric complex with ssdna (Complex from synthetic construct)
- Replication protein a heterotrimeric complex with ssdna (120 kDa, Complex)
- Dna (5'-d(p*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*t)-3') (6 kDa, DNA from synthetic construct)
Cryo-EM structure of type III-A Csm-CTR1 complex, AMPPNP bound
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6ifk
Q-score: 0.511
You L, Ma J, Wang J, Artamonova D, Wang M, Liu L, Xiang H, Severinov K, Zhang X, Wang Y
Cell (2019) 176 pp. 239-253.e16 [ Pubmed: 30503210 DOI: doi:10.1016/j.cell.2018.10.052 ]
- Zinc ion (65 Da, Ligand)
- Ctr1 (13 kDa, RNA from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated protein csm2 (14 kDa, Protein from Streptococcus thermophilus ND03)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Type iii-a crispr-associated protein csm1 (86 kDa, Protein from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated ramp protein csm3 (24 kDa, Protein from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated ramp protein csm5 (41 kDa, Protein from Streptococcus thermophilus ND03)
- Crrna (10 kDa, RNA from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated ramp protein csm4 (33 kDa, Protein from Streptococcus thermophilus ND03)
- Csm-ctr1 complex, amppnp bound (290 kDa, Complex from Streptococcus thermophilus ND03)
Cryo-EM structure of type III-A Csm-NTR complex
Resolution: 3.16 Å
EM Method: Single-particle
Fitted PDBs: 6ifl
Q-score: 0.5
You L, Ma J, Wang J, Artamonova D, Wang M, Liu L, Xiang H, Severinov K, Zhang X, Wang Y
Cell (2019) 176 pp. 239-253.e16 [ Pubmed: 30503210 DOI: doi:10.1016/j.cell.2018.10.052 ]
- Csm1 (Complex from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated ramp protein csm4 (33 kDa, Protein from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated protein csm2 (14 kDa, Protein from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated protein csm1 (86 kDa, Protein from Streptococcus thermophilus ND03)
- Csm-ctr1 complex, amppnp bound (87 kDa, Complex from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated ramp protein csm5 (41 kDa, Protein from Streptococcus thermophilus)
- Crrna (11 kDa, RNA from Streptococcus thermophilus ND03)
- Ntr (13 kDa, RNA from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated ramp protein csm3 (24 kDa, Protein from Streptococcus thermophilus ND03)
Cryo-EM structure of type III-A Csm complex
You L, Ma J, Wang J, Artamonova D, Wang M, Liu L, Xiang H, Severinov K, Zhang X, Wang Y
Cell (2019) 176 pp. 239-253.e16 [ Pubmed: 30503210 DOI: doi:10.1016/j.cell.2018.10.052 ]
- Csm-ctr1 complex, amppnp bound (290 kDa, Complex from Streptococcus thermophilus ND03)
Type III-A Csm complex, Cryo-EM structure of Csm-NTR, ATP bound
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6ifr
Q-score: 0.456
You L, Ma J, Wang J, Artamonova D, Wang M, Liu L, Xiang H, Severinov K, Zhang X, Wang Y
Cell (2019) 176 pp. 239-253.e16 [ Pubmed: 30503210 DOI: doi:10.1016/j.cell.2018.10.052 ]
- Type iii-a crispr-cas interference complex, crrna (11 kDa, RNA from Streptococcus thermophilus ND03)
- Csm-ntr complex, atp bound (290 kDa, Complex from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated protein csm2 (14 kDa, Protein from Streptococcus thermophilus ND03)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Type iii-a crispr-associated protein csm1 (86 kDa, Protein from Streptococcus thermophilus ND03)
- Type iii-a crispr-cas interference complex, ntr (13 kDa, RNA from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated ramp protein csm3 (24 kDa, Protein from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated ramp protein csm5 (41 kDa, Protein from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated ramp protein csm4 (33 kDa, Protein from Streptococcus thermophilus ND03)
Cryo-EM structure of type III-A Csm-CTR2-dsDNA complex
Resolution: 3.05 Å
EM Method: Single-particle
Fitted PDBs: 6ifu
Q-score: 0.508
You L, Ma J, Wang J, Artamonova D, Wang M, Liu L, Xiang H, Severinov K, Zhang X, Wang Y
Cell (2019) 176 pp. 239-253.e16 [ Pubmed: 30503210 DOI: doi:10.1016/j.cell.2018.10.052 ]
- Type iii-a crispr-associated ramp protein csm5 (41 kDa, Protein from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated ramp protein csm4 (33 kDa, Protein from Streptococcus thermophilus ND03)
- Type iii-a csm-ctr2-dsdna complex (300 kDa, Complex from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated protein csm2 (14 kDa, Protein from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated ramp protein csm3 (24 kDa, Protein from Streptococcus thermophilus ND03)
- Crrna (11 kDa, RNA from Streptococcus thermophilus ND03)
- Ctr2 (16 kDa, RNA from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated protein csm1 (86 kDa, Protein from Streptococcus thermophilus ND03)
- Type iii-a csm-ctr2-dsdna complex, csm1 subunit (Complex from Streptococcus thermophilus ND03)
Type III-A Csm complex, Cryo-EM structure of Csm-CTR1
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6ify
Q-score: 0.379
You L, Ma J, Wang J, Artamonova D, Wang M, Liu L, Xiang H, Severinov K, Zhang X, Wang Y
Cell (2019) 176 pp. 239-253.e16 [ Pubmed: 30503210 DOI: doi:10.1016/j.cell.2018.10.052 ]
- Csm1 (Complex from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated ramp protein csm5 (41 kDa, Protein from Streptococcus thermophilus ND03)
- Csm-ctr1 complex (87 kDa, Complex from Streptococcus thermophilus ND03)
- Ctr1 (13 kDa, RNA from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated protein csm2 (14 kDa, Protein from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated protein csm1 (86 kDa, Protein from Streptococcus thermophilus ND03)
- Crrna (11 kDa, RNA from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated ramp protein csm4 (33 kDa, Protein from Streptococcus thermophilus)
- Type iii-a crispr-associated ramp protein csm3 (24 kDa, Protein from Streptococcus thermophilus ND03)
- Zinc ion (65 Da, Ligand)
Type III-A Csm complex, Cryo-EM structure of Csm-CTR2-ssDNA complex
Resolution: 3.58 Å
EM Method: Single-particle
Fitted PDBs: 6ifz
Q-score: 0.475
You L, Ma J, Wang J, Artamonova D, Wang M, Liu L, Xiang H, Severinov K, Zhang X, Wang Y
Cell (2019) 176 pp. 239-253.e16 [ Pubmed: 30503210 DOI: doi:10.1016/j.cell.2018.10.052 ]
- Type iii-a crispr-associated ramp protein csm5 (41 kDa, Protein from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated ramp protein csm4 (33 kDa, Protein from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated protein csm2 (14 kDa, Protein from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated protein csm1 (86 kDa, Protein from Streptococcus thermophilus ND03)
- Crrna (11 kDa, RNA from Streptococcus thermophilus ND03)
- Ctr2 (16 kDa, RNA from Streptococcus thermophilus ND03)
- Type iii-a crispr-cas interference complex subunit csm1 (Complex from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated ramp protein csm3 (24 kDa, Protein from Streptococcus thermophilus ND03)
- Zinc ion (65 Da, Ligand)
- Csm-ctr2-ssdna complex (87 kDa, Complex from Streptococcus thermophilus ND03)
Type III-A Csm complex, Cryo-EM structure of Csm-CTR1, ATP bound
Resolution: 3.37 Å
EM Method: Single-particle
Fitted PDBs: 6ig0
Q-score: 0.461
You L, Ma J, Wang J, Artamonova D, Wang M, Liu L, Xiang H, Severinov K, Zhang X, Wang Y
Cell (2019) 176 pp. 239-253.e16 [ Pubmed: 30503210 DOI: doi:10.1016/j.cell.2018.10.052 ]
- Csm-ctr1 complex, atp bound (290 kDa, Complex from Streptococcus thermophilus ND03)
- Ctr1 (13 kDa, RNA from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated protein csm2 (14 kDa, Protein from Streptococcus thermophilus ND03)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Type iii-a crispr-associated protein csm1 (86 kDa, Protein from Streptococcus thermophilus ND03)
- Crrna (11 kDa, RNA from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated ramp protein csm3 (24 kDa, Protein from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated ramp protein csm5 (41 kDa, Protein from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated ramp protein csm4 (33 kDa, Protein from Streptococcus thermophilus ND03)
- Zinc ion (65 Da, Ligand)