GPR3 Orphan G-coupled Protein Receptor in complex with Dominant Negative Gs.
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 8u8f
Q-score: 0.422
Russell IC, Zhang X, Bumbak F, McNeill SM, Josephs TM, Leeming MG, Christopoulos G, Venugopal H, Flocco MM, Sexton PM, Wootten D, Belousoff MJ
Biochemistry (2024) 63 pp. 625-631 [ Pubmed: 38376112 DOI: doi:10.1021/acs.biochem.3c00647 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Active state, gpr3 complex with dominant negative g-alphas. (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- G-protein coupled receptor 3 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
Exendin-4-bound Glucagon-Like Peptide-1 (GLP-1) Receptor in complex with Gs protein
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7lll
Q-score: 0.479
Deganutti G, Liang YL, Zhang X, Khoshouei M, Clydesdale L, Belousoff MJ, Venugopal H, Truong TT, Glukhova A, Keller AN, Gregory KJ, Leach K, Christopoulos A, Danev R, Reynolds CA, Zhao P, Sexton PM, Wootten D
Nat Commun (2022) 13 pp. 92-92 [ Pubmed: 35013280 DOI: doi:10.1038/s41467-021-27760-0 ]
- Nb35 (13 kDa, Protein from Lama glama)
- Exendin-4-glp-1r-gs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Exendin-4 (4 kDa, Protein from Heloderma suspectum)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
Oxyntomodulin-bound Glucagon-Like Peptide-1 (GLP-1) Receptor in complex with Gs protein
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7lly
Q-score: 0.452
Deganutti G, Liang YL, Zhang X, Khoshouei M, Clydesdale L, Belousoff MJ, Venugopal H, Truong TT, Glukhova A, Keller AN, Gregory KJ, Leach K, Christopoulos A, Danev R, Reynolds CA, Zhao P, Sexton PM, Wootten D
Nat Commun (2022) 13 pp. 92-92 [ Pubmed: 35013280 DOI: doi:10.1038/s41467-021-27760-0 ]
- Nb35 (13 kDa, Protein from Lama glama)
- Pro-glucagon (4 kDa, Protein from Sus scrofa)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Oxyntomodulin-glp-1r-gs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)
Resolution: 2.14 Å
EM Method: Single-particle
Fitted PDBs: 7rtb
Q-score: 0.533
Johnson RM, Zhang X, Piper SJ, Nettleton TJ, Vandekolk TH, Langmead CJ, Danev R, Sexton PM, Wootten D
Biochem Biophys Res Commun (2021) 578 pp. 84-90 [ Pubmed: 34547628 DOI: doi:10.1016/j.bbrc.2021.09.016 ]
- Water (18 Da, Ligand)
- Nb35 (13 kDa, Protein from Lama glama)
- Peptide-19-glp-1r-gs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Peptide-19 (4 kDa, Protein from synthetic construct)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
Semaglutide-bound Glucagon-Like Peptide-1 (GLP-1) Receptor in Complex with Gs protein
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7ki0
Q-score: 0.557
Zhang X, Belousoff MJ, Liang YL, Danev R, Sexton PM, Wootten D
Cell Rep (2021) 36 pp. 109374-109374 [ DOI: doi:10.1016/j.celrep.2021.109374 Pubmed: 34260945 ]
- Water (18 Da, Ligand)
- Nb35 (13 kDa, Protein from Lama glama)
- 17-amino-10-oxo-3,6,12,15-tetraoxa-9-azaheptadecan-1-oic acid (308 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Semaglutide-glp-1r-gs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Semaglutide (3 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
Taspoglutide-bound Glucagon-Like Peptide-1 (GLP-1) Receptor in Complex with Gs Protein
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7ki1
Q-score: 0.535
Zhang X, Belousoff MJ, Liang YL, Danev R, Sexton PM, Wootten D
Cell Rep (2021) 36 pp. 109374-109374 [ DOI: doi:10.1016/j.celrep.2021.109374 Pubmed: 34260945 ]
- Taspoglutide (3 kDa, Protein from synthetic construct)
- Nb35 (13 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Taspoglutide-glp-1r-gs complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
PF 06882961 bound to the glucagon-like peptide-1 receptor (GLP-1R):Gs complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7lci
Q-score: 0.428
Zhang X, Johnson RM, Drulyte I, Yu L, Kotecha A, Danev R, Wootten D, Sexton PM, Belousoff MJ
Structure (2021) 29 pp. 963- [ Pubmed: 33957078 DOI: doi:10.1016/j.str.2021.04.008 ]
- Glp-1:gs complex with small molecule agonist bound (pf-06882961) (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- 2-[(4-{6-[(4-cyano-2-fluorophenyl)methoxy]pyridin-2-yl}piperidin-1-yl)methyl]-1-{[(2s)-oxetan-2-yl]methyl}-1h-benzimidazole-6-carboxylic acid (555 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
PF 06882961 bound to the glucagon-like peptide-1 receptor (GLP-1R):Gs complex
Resolution: 2.82 Å
EM Method: Single-particle
Fitted PDBs: 7lcj
Q-score: 0.533
Zhang X, Johnson RM, Drulyte I, Yu L, Kotecha A, Danev R, Wootten D, Sexton PM, Belousoff MJ
Structure (2021) 29 pp. 963- [ Pubmed: 33957078 DOI: doi:10.1016/j.str.2021.04.008 ]
- 2-[(4-{6-[(4-cyano-2-fluorophenyl)methoxy]pyridin-2-yl}piperidin-1-yl)methyl]-1-{[(2s)-oxetan-2-yl]methyl}-1h-benzimidazole-6-carboxylic acid (555 Da, Ligand)
- Receptor only portion of glp1:gs complex with a small molecule agonist bound: pf-06882961 (Complex from Homo sapiens)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
PF 06882961 bound to the glucagon-like peptide-1 receptor (GLP-1R)
Resolution: 3.24 Å
EM Method: Single-particle
Fitted PDBs: 7lck
Q-score: 0.477
Zhang X, Johnson RM, Drulyte I, Yu L, Kotecha A, Danev R, Wootten D, Sexton PM, Belousoff MJ
Structure (2021) 29 pp. 963- [ Pubmed: 33957078 DOI: doi:10.1016/j.str.2021.04.008 ]
- Water (18 Da, Ligand)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Glp-1:gs complex with small molecule agonist (pf-06882961) bound (Complex from Homo sapiens)
- 2-[(4-{6-[(4-cyano-2-fluorophenyl)methoxy]pyridin-2-yl}piperidin-1-yl)methyl]-1-{[(2s)-oxetan-2-yl]methyl}-1h-benzimidazole-6-carboxylic acid (555 Da, Ligand)
GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
Resolution: 2.1 Å
EM Method: Single-particle
Fitted PDBs: 6x18
Q-score: 0.563
Zhang X, Belousoff MJ, Zhao P, Kooistra AJ, Truong TT, Ang SY, Underwood CR, Egebjerg T, Senel P, Stewart GD, Liang YL, Glukhova A, Venugopal H, Christopoulos A, Furness SGB, Miller LJ, Reedtz-Runge S, Langmead CJ, Gloriam DE, Danev R, Sexton PM, Wootten D
Mol Cell (2020) 80 pp. 485- [ Pubmed: 33027691 DOI: doi:10.1016/j.molcel.2020.09.020 ]
- Water (18 Da, Ligand)
- Glp-1 peptide hormone bound to glucagon-like peptide-1 (glp-1) receptor (120 kDa, Complex)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, glucagon-like peptide 1 receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Nanobody 35 (Complex from Lama glama)
- Nanobody35 (13 kDa, Protein from Lama glama)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Glucagon (3 kDa, Protein from Homo sapiens)