cryo-EM structure of NTSR1-GRK2-Galpha(q) complexes 1
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 8jpb
Q-score: 0.448
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ Pubmed: 37532940 DOI: doi:10.1038/s41586-023-06395-9 ]
- Nts(8-13) (819 Da, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(q) subunit alpha (41 kDa, Protein from Homo sapiens)
- Staurosporine (466 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- 2-[{2-(1-fluorocyclopropyl)-4-[4-(2-methoxyphenyl)piperidin-1-yl]quinazolin-6-yl}(methyl)amino]ethan-1-ol (450 Da, Ligand)
- Ntsr1-grk2-galpha(q) complexes 1 (Complex from Homo sapiens)
- Neurotensin receptor type 1 (46 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Beta-adrenergic receptor kinase 1 (79 kDa, Protein from Bos taurus)
- Tetrafluoroaluminate ion (102 Da, Ligand)
cryo-EM structure of NTSR1-GRK2-Galpha(q) complexes 2
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 8jpc
Q-score: 0.458
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ Pubmed: 37532940 DOI: doi:10.1038/s41586-023-06395-9 ]
- Nts(8-13) (819 Da, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(q) subunit alpha (41 kDa, Protein from Homo sapiens)
- Staurosporine (466 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Ntsr1-grk2-galpha(q) complexes 2 (Complex from Homo sapiens)
- 2-[{2-(1-fluorocyclopropyl)-4-[4-(2-methoxyphenyl)piperidin-1-yl]quinazolin-6-yl}(methyl)amino]ethan-1-ol (450 Da, Ligand)
- Neurotensin receptor type 1 (46 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Beta-adrenergic receptor kinase 1 (79 kDa, Protein from Bos taurus)
- Tetrafluoroaluminate ion (102 Da, Ligand)
Focused refinement structure of GRK2 in NTSR1-GRK2-Galpha(q) complexes
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 8jpd
Q-score: 0.497
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ Pubmed: 37532940 DOI: doi:10.1038/s41586-023-06395-9 ]
- Focused refinement structure of grk2 in ntsr1-grk2-galpha(q) complexes (Complex from Bos taurus)
- Beta-adrenergic receptor kinase 1 (79 kDa, Protein from Bos taurus)
- Staurosporine (466 Da, Ligand)
Focused refinement structure of Galpha(q) in NTSR1-GRK2-Galpha(q) complexes
Resolution: 2.91 Å
EM Method: Single-particle
Fitted PDBs: 8jpe
Q-score: 0.447
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ Pubmed: 37532940 DOI: doi:10.1038/s41586-023-06395-9 ]
- Guanine nucleotide-binding protein g(q) subunit alpha (41 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Focused refinement structure of galpha(q) in ntsr1-grk2-galpha(q) complexes (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Tetrafluoroaluminate ion (102 Da, Ligand)
Focused refiment structure of NTSR1 in NTSR1-GRK2-Galpha(q) complexes
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 8jpf
Q-score: 0.479
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ Pubmed: 37532940 DOI: doi:10.1038/s41586-023-06395-9 ]
- Focused refiment structure of ntsr1 in ntsr1-grk2-galpha(q) complexes (Complex from Homo sapiens)
- Neurotensin receptor type 1 (46 kDa, Protein from Homo sapiens)
- Nts (819 Da, Protein from synthetic construct)
- 2-[{2-(1-fluorocyclopropyl)-4-[4-(2-methoxyphenyl)piperidin-1-yl]quinazolin-6-yl}(methyl)amino]ethan-1-ol (450 Da, Ligand)
Cryo-EM structure of IRL1620-bound ETBR-Gi complex
Resolution: 2.99 Å
EM Method: Single-particle
Fitted PDBs: 8hbd
Q-score: 0.412
Ji Y, Duan J, Yuan Q, He X, Yang G, Zhu S, Wu K, Hu W, Gao T, Cheng X, Jiang H, Eric Xu H, Jiang Y
Nat Commun (2023) 14 pp. 1268-1268 [ DOI: doi:10.1038/s41467-023-36998-9 Pubmed: 36882417 ]
- Irl1620-bound etbr-gi complex (Complex from Homo sapiens)
- Endothelin receptor type b,endothelin receptor type b,oplophorus-luciferin 2-monooxygenase catalytic subunit chimera (67 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Irl1620 (1 kDa, Protein from Homo sapiens)
Cryo-EM structure of endothelin1-bound ETAR-Gq complex
Resolution: 3.01 Å
EM Method: Single-particle
Fitted PDBs: 8hcq
Q-score: 0.394
Ji Y, Duan J, Yuan Q, He X, Yang G, Zhu S, Wu K, Hu W, Gao T, Cheng X, Jiang H, Eric Xu H, Jiang Y
Nat Commun (2023) 14 pp. 1268-1268 [ DOI: doi:10.1038/s41467-023-36998-9 Pubmed: 36882417 ]
- Guanine nucleotide-binding protein g(q) subunit alpha-1 (28 kDa, Protein from Homo sapiens)
- Endothelin-1 (2 kDa, Protein from Homo sapiens)
- Endothelin-1 receptor,oplophorus-luciferin 2-monooxygenase catalytic subunit chimera (69 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Et1-etar-gq-scfv16 complex (Complex from Homo)
Cryo-EM structure of Endothelin1-bound ETBR-Gq complex
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8hcx
Q-score: 0.346
Ji Y, Duan J, Yuan Q, He X, Yang G, Zhu S, Wu K, Hu W, Gao T, Cheng X, Jiang H, Eric Xu H, Jiang Y
Nat Commun (2023) 14 pp. 1268-1268 [ DOI: doi:10.1038/s41467-023-36998-9 Pubmed: 36882417 ]
- Endothelin receptor type b,oplophorus-luciferin 2-monooxygenase catalytic subunit chimera (67 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(q) subunit alpha-1 (28 kDa, Protein from Homo sapiens)
- Endothelin-1 (2 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Et1-etar-gq-scfv16 complex (Complex from Homo)
Lodoxamide-bound GPR35 in complex with G13
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8h8j
Q-score: 0.416
Duan J, Liu Q, Yuan Q, Ji Y, Zhu S, Tan Y, He X, Xu Y, Shi J, Cheng X, Jiang H, Eric Xu H, Jiang Y
Cell Discov (2022) 8 pp. 135-135 [ DOI: doi:10.1038/s41421-022-00499-8 Pubmed: 36543774 ]
- G-protein coupled receptor 35 (33 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Lodoxamide-gpr35-g13 complex (Complex from Homo sapiens)
- 2,2'-[(2-chloro-5-cyano-1,3-phenylene)bis(azanediyl)]bis(oxoacetic acid) (311 Da, Ligand)
- Scfv16 (26 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein subunit alpha-13 (42 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)